Cited by 29Plant tissue culture and regenerationFlowering Plant Growth and CultivationPlant Molecular Biology Research

Flow Cytometry and Start Codon Targeted (SCoT) Genetic Fidelity Assessment of Regenerated Plantlets in Tylophora indica (Burm.f.) Merrill

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Mamgain, Jyoti, Mujib, A., Ejaz, Bushra, Gulzar, Basit, Malik, Moien Qadir, Syeed, Rukaya·2022·Plant Cell, Tissue and Organ Culture (PCTOC)

Abstract

Tylophora indica (Burm.f.) Merrill. is a pharmacologically important plant, popular for alkaloidal and non-alkaloidal richness. Large scale propagation of T. indica is difficult in the wild as the seeds are small and the frequency of germination is very poor. In the present study, the genome size estimation of in vitro regenerated (indirect, direct and somatic embryo mediated) T. indica was made by flow cytometric method. Clonal fidelity of the regenerants was assessed using a start codon targeted (SCoT) molecular marker. Initially, the explants were inoculated on Murashige and Skoog basal medium supplemented with various concentrations of plant growth regulators like 2,4-dichlorophenoxy acetic acid (2,4-D), Kinetin, 6-benzyl amino purine (BAP) and 1-naphthalene acetic acid either singly or in combinations. The highest callus induction frequency (87.75%) was obtained in 6.7 µM 2,4-D added MS medium which metamorphosed into progressive stages (globular, heart, torpedo, and cotyledonary) of embryos. Mature and healthy somatic embryos efficiently germinated into plantlets on 8.8 µM BAP + 1.4 µM GA 3 enriched MS medium. Histological and scanning electron microscopic study confirmed the above developing stages. The regenerated shoots were rooted best in 2.45 µM Indole-3-butyric acid supplemented solid MS medium. The plants were hardened and acclimatized with 90% survivability. The flow cytometric 2C DNA content of indirect, direct and somatic embryo derived plants was 1.896 pg, 1.940 pg and 1.926 pg respectively, very similar to the mother plant (1.928 pg). SCoT marker generated a high percentage of monomorphic bands (94%) revealing similarity with the mother plant, thus ensuring genetic fidelity. To the best of our knowledge, this is perhaps the first ever report of 2C DNA content estimation and SCoT marker based genetic homogeneity study in T. indica. Supplementary information The online version contains supplementary material available at 10.1007/s11240-022-02254-z.

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  • In Tylophora indica, the SCoT marker produced a high percentage of monomorphic bands (94%), indicating genetic fidelity and similarity between regenerated and mother plants.

    “In Tylophora indica, the SCoT marker produced a high percentage of monomorphic bands (94%), indicating genetic fidelity and similarity between regenerated and mother plants [131].”

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Research works printed but not linked: 4

  1. Beyene B Beyene B Deribe H Review on application and management of medicinal plants for the livelihood of the local community J Res Dev Mange 2016 22 33 39
  2. Dolezel J Nuclear DNA content and genome size of trout and human Cytom Part A 2003 51 127 128 12541287
  3. Johansen DA Plant microtechnique 1940 New York, NY McGraw-Hill Book Co.
  4. Sah NK Prasad BD Scope of plant biotechnology in the developing countries Plant biotechnology 2017 Palm Bay, FL Apple Academic Press 45 66

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Authors
Mamgain, J., Mujib, A., Ejaz, B., Gulzar, B., Malik, M. Q., & Syeed, R.
Affiliations
Jamia Hamdard (IN)
Corresponding author
Mujib, A.
Venue
Plant Cell, Tissue and Organ Culture (PCTOC)
Published
2022-02-28
Volume
150
Issue
1
Pages
129-140
ISSN
  • 0167-6857 (ISSN-L)
  • 1573-5044
OpenAlex
W4214664472
Cited by
29
References
47
Citations by year
2026: 3 · 2025: 8 · 2024: 4 · 2023: 11 · 2022: 3
FWCI
2.12
Type
journal article
Language
en
Open access
Locations
  • Plant Cell Tissue and Organ Culture (PCTOC) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
Hamdard National Foundation
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant tissue culture and regeneration
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Flowering Plant Growth and Cultivation
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
Keywords
Tylophora indica, somatic embryogenesis, plant regeneration, clonal fidelity, genome size estimation, 2C DNA content, callus induction, plant growth regulators, 2,4-D, benzyladenine, indole-3-butyric acid, acclimatization

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