The DOI matches its Crossref record; 7 of the 7 references it cites were found in a registry or an index.
Hemizygous minipigs produced by random gene insertion and handmade cloning express the Alzheimer’s disease-causing dominant mutation APPsw
Open accessAbstract
In an effort to develop a porcine model of Alzheimer’s disease we used handmade cloning to produce seven transgenic Göttingen minipigs. The donor fibroblasts had been stably transfected with a plasmid cassette containing, as transgene, the cDNA of the neuronal variant of the human amyloid precursor protein gene with the Swedish mutation preceded by beta-globin sequences to induce splicing and a human PDGFbeta promoter fragment to drive transcription. Transgene insertion had occurred only at the GLIS3 locus where a single complete copy of the transgene was identified in intronic sequences in opposite direction. Similar and robust levels of the transgene transcript were detected in skin biopsies from all piglets and the sequence of full-length transcript was verified. Consistent with PDGFbeta promoter function, high levels of transgene expression, including high level of the corresponding protein, was observed in brain tissue and not in heart or liver tissues. A rough estimate predicts that accumulation of the Aβ peptide in the brain may develop at the age of 1–2 years.
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Triple-Transgenic Model of Alzheimer’s Disease with Plaques and Tangles
Oddo, S., Caccamo, A., Shepherd, J. D., Murphy, M. P., Golde, T. E., Kayed, R., Metherate, R., Mattson, M. P., Akbari, Y., & LaFerla, F. M. (2003) · Neuron · doi:10.1016/s0896-6273(03)00434-3 · Cited by 4,126
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Alzheimer-type neuropathology in transgenic mice overexpressing V717F β-amyloid precursor protein
Games, D., Adams, D., Alessandrini, R., Barbour, R., Borthelette, P., Blackwell, C., Carr, T., Clemens, J., Donaldson, T., Gillespie, F., et al. (1995) · Nature · doi:10.1038/373523a0 · Cited by 2,516
Mullan, M., Crawford, F., Axelman, K., Houlden, H., Lilius, L., Winblad, B., & Lannfelt, L. (1992) · Nature Genetics · doi:10.1038/ng0892-345 · Cited by 1,513
Borchelt, D. R., Ratovitski, T., van Lare, J., Lee, M. K., Gonzales, V., Jenkins, N. A., Copeland, N. G., Price, D. L., & Sisodia, S. S. (1997) · Neuron · doi:10.1016/s0896-6273(00)80974-5 · Cited by 1,023
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Takeuchi, A., Irizarry, M. C., Duff, K., Saido, T. C., Hsiao Ashe, K., Hasegawa, M., Mann, D. M. A., Hyman, B. T., & Iwatsubo, T. (2000) · The American Journal of Pathology · doi:10.1016/s0002-9440(10)64544-0 · Cited by 229
Cited by
Porcine Models of Cutaneous Wound Healing
Seaton, M., Hocking, A., & Gibran, N. S. (2015) · ILAR Journal · doi:10.1093/ilar/ilv016 · Cited by 249
The pig as an animal model for human pathologies: A proteomics perspective
Bassols, A., Costa, C., Eckersall, P. D., Osada, J., Sabrià, J., & Tibau, J. (2014) · PROTEOMICS – Clinical Applications · doi:10.1002/prca.201300099 · Cited by 254
Genetically engineered pigs as models for human disease
Perleberg, C., Kind, A., & Schnieke, A. (2018) · Disease Models & Mechanisms · doi:10.1242/dmm.030783 · Cited by 222
Genetically modified large animal models for investigating neurodegenerative diseases
Yang, W., Chen, X., Li, S., & Li, X.-J. (2021) · Cell & Bioscience · doi:10.1186/s13578-021-00729-8 · Cited by 45
Metadata
- Authors
- Kragh, P. M., Nielsen, A. L., Li, J., Du, Y., Lin, L., Schmidt, M., Bøgh, I. B., Holm, I. E., Jakobsen, J. E., Johansen, M. G., Purup, S., Bolund, L., Vajta, G., & Jørgensen, A. L.
- Venue
- Transgenic Research
- Published
- 2009-01-29
- Volume
- 18
- Issue
- 4
- Pages
- 545-558
- ISSN
- 0962-8819 (ISSN-L)
- 1573-9368
- OpenAlex
- W1982078790
- Cited by
- 174
- References
- 7
- FWCI
- 9.98
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Hemizygous minipigs produced by random gene insertion and handmade cloning express the Alzheimer’s disease-causing dominant mutation APPsw. https://app.cochat.ai/papers/10.1007/s11248-009-9245-4
- The paper
- Kragh, P. M., Nielsen, A. L., Li, J., Du, Y., Lin, L., Schmidt, M., Bøgh, I. B., Holm, I. E., Jakobsen, J. E., Johansen, M. G., Purup, S., Bolund, L., Vajta, G., & Jørgensen, A. L. (2009). Hemizygous minipigs produced by random gene insertion and handmade cloning express the Alzheimer’s disease-causing dominant mutation APPsw. Transgenic Research. https://doi.org/10.1007/s11248-009-9245-4
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
