The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Impact of chronic exposure to low doses of chlorpyrifos on the intestinal microbiota in the Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and in the rat
Abstract
The impact of the insecticide chlorpyrifos (CPF) on the mammalian digestive system has been poorly described. The present study aimed at evaluating the effect of chronic, low-dose exposure to CPF on the composition of the gut microbiota in a Simulator of the Human Intestinal Microbial Ecosystem: the SHIME and in rats. The SHIME comprises six reactor vessels (stomach to colon). The colonic segments were inoculated with feces from healthy humans. Then, the simulator was exposed to a daily dose of 1 mg of CPF for 30 days. The changes over time in the populations of bacteria were examined at different time points: prior to pesticide exposure (as a control) and after exposure. In parallel, pregnant rats were gavaged daily with 1 mg/kg of CPF (or vehicle) until the pups were weaned. Next, the rats were gavaged with same dose of CPF until 60 days of age (adulthood). Then, samples of different parts of the digestive tract were collected under sterile conditions for microbiological assessment. Chronic, low-dose exposure to CPF in the SHIME and in the rat was found to induce dysbiosis in the microbial community with, in particular, proliferation of subpopulations of some strains and a decrease in the numbers of others bacteria. In compliance with European guidelines, the use of the SHIME in vitro tool would help to (1) elucidate the final health effect of toxic agents and (2) minimize (though not fully replace) animal testing. Indeed, certain parameters would still have to be studied further in vivo.
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“Chronic exposure to low doses of the insecticide, Chlorpyrifos, changed the composition of the gut microbiota in both a simulated human intestinal microbiota preparation and rats, reducing the abundance of Lactobacillus spp.and Bifidobacterium spp, bacteria presumed to be beneficial (Joly et al., 2013).”
From Gut reactions: Breaking down xenobiotic–microbiome interactions (2019) · cites this paper as Joly et al., 2013
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Research works printed but not linked: 1
- Agence nationale de sécurité sanitaire de l’alimentation de l’environnement et du travail (2011) Résultats de l’étude nationale de surveillance des expositions alimentaires aux substances chimiques. Etude l’Alimentation Totale 2:2006–2010
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Ciaula, A. D., Garruti, G., Baccetto, R., Molina‐Molina, E., Bonfrate, L., Wang, D. Q. ‐., & Portincasa, P. (2017) · Annals of Hepatology · doi:10.5604/01.3001.0010.5493 · Cited by 598
Effects of Psychological, Environmental and Physical Stressors on the Gut Microbiota
Karl, J. P., Hatch‐McChesney, A., Arcidiacono, S. M., Pearce, S., Pantoja-Feliciano, I. G., Doherty, L. A., & Soares, J. W. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.02013 · Cited by 563
Djoumbou-Feunang, Y., Fiamoncini, J., Gil-de-la-Fuente, A., Greiner, R., Manach, C., & Wishart, D. S. (2019) · Journal of Cheminformatics · doi:10.1186/s13321-018-0324-5 · Cited by 531
Metadata
- Authors
- Joly, C., Gay-Quéheillard, J., Léké, A., Chardon, K., Delanaud, S., Bach, V., & Khorsi-Cauet, H.
- Affiliations
- Institut national de l'environnement industriel et des risques (FR)
- Venue
- Environmental Science and Pollution Research
- Published
- 2012-11-08
- Volume
- 20
- Issue
- 5
- Pages
- 2726-2734
- ISSN
- 0944-1344 (ISSN-L)
- 1614-7499
- OpenAlex
- W2095549625
- Cited by
- 135
- References
- 47
- Citations by year
- 2026: 4 · 2025: 13 · 2024: 13 · 2023: 5 · 2022: 10 · 2021: 15 · 2020: 10 · 2019: 16 · 2018: 15 · 2017: 12 · 2016: 7 · 2015: 8 · 2014: 6 · 2013: 1
- FWCI
- 1.93
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Environmental Science and Pollution Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Conseil Régional de Picardie
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Pesticide Exposure and Toxicity
- Life Sciences › Immunology and Microbiology › Immunology › Immunotoxicology and immune responses
- Keywords
- chlorpyrifos, gut microbiota, pesticide exposure, chronic low-dose exposure, dysbiosis, SHIME, in vitro gut model, rat model, intestinal bacteria, alternatives to animal testing
- MeSH terms
- Animals, Dose-Response Relationship, Drug, Chlorpyrifos, Environmental Exposure, Environmental Monitoring, Environmental Pollutants, Female, Humans, Insecticides, Intestines, Male, Models, Biological, Time Factors, Rats, Metagenome
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