Protective role of a methanolic extract of spinach (Spinacia oleracea L.) against Pb toxicity in wheat (Triticum aestivum L.) seedlings: Beneficial effects for a plant of a nutraceutical used with animals
Abstract
Spinach extracts contain powerful natural antioxidants and have been used to improve the response of animal cells to various stress factors. The aim of the present study was to assess the effects of a methanolic extract of spinach (SE) used at two concentrations (21.7 and 217 ppm) on the growth, certain enzymes and antioxidant systems in wheat seedlings under lead stress. When wheat seedlings were grown for 7 days in a solution containing Pb(NO3)2 (3 mM), germination and growth were impaired, while signs of oxidative stress were observed. SE (217 ppm) pretreatment was able to protect seedlings from Pb toxicity by both reducing Pb uptake and Pb-induced oxidative stress. As a consequence, almost normal germination, elongation, biomass and α-amylase activity were restored by SE (217 ppm) pretreatment of wheat seedlings, in spite of the presence of Pb. Our results support the protective role and the antioxidant effect of SE against Pb. These results show an amazing similarity to the effects of SE in animals, which suggests that providing "nutraceuticals" to plants could improve their "health" status.
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In Spinacia oleracea roots, PE production increases during pathogenic attack.
“In Spinacia oleracea roots, it has been found that PE production increases during pathogenic attack [21,22,149,150,151].”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [149]
PE biosynthesis increases during mechanical wounding or pathogenic attack.
“PEs provide plants tolerance against several soil nematodes and insects; thus, PE biosynthesis increases during mechanical wounding or pathogenic attack.”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [149]
In spinach, 20-HE plays a potent role in enhancing stress tolerance by stimulating the activity of antioxidant enzymes, proteins, and proline, mitigating environmental stress and enhancing plant metabolic responses to respond better to any abiotic stress.
“In spinach, 20-HE plays a potent role in enhancing stress tolerance. It stimulates the activity of antioxidant enzymes, proteins, and proline, mitigating environmental stress and enhancing plant metabolic responses to respond better to any abiotic stress [149].”
From Phytoecdysteroids: Distribution, Structural Diversity, Biosynthesis, Activity, and Crosstalk with Phytohormones (2022) · cites this paper as [149]
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References 46 · Cited by 15
- Authors
- Lamhamdi, M., Bakrim, A., Bouayad, N., Aarab, A., & Lafont, R.
- Affiliations
- Abdelmalek Essaâdi University (MA)
- Université Pierre-et-Marie-Curie (FR)
- Corresponding author
- Bakrim, Ahmed
- Venue
- Environmental Science and Pollution Research
- Published
- 2013-05-05
- Volume
- 20
- Issue
- 10
- Pages
- 7377-7385
- ISSN
- 0944-1344 (ISSN-L)
- 1614-7499
- OpenAlex
- W2003706039
- Semantic Scholar
- 8e9b625cd48b92a19ad6a89633143a67286891d4
- Cited by
- 15
- References
- 46
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 1 · 2019: 2 · 2018: 1 · 2017: 1 · 2015: 1
- FWCI
- 0.40
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Environmental Science and Pollution Research (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Physical Sciences › Environmental Science › Pollution › Heavy metals in environment
- Physical Sciences › Environmental Science › Health, Toxicology and Mutagenesis › Heavy Metal Exposure and Toxicity
- Keywords
- spinach extract, spinach, lead toxicity, wheat seedlings, wheat, oxidative stress, antioxidant enzymes, lead uptake, seed germination, alpha-amylase, heavy metal stress
- MeSH terms
- Animals, Antioxidants, Catalase, Lead, Malondialdehyde, Plant Extracts, Soil Pollutants, Superoxide Dismutase, Triticum, Oxidative Stress, Germination, Spinacia oleracea, Dietary Supplements, Seedlings, Ascorbate Peroxidases
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