The DOI matches its Crossref record; 86 of the 86 references it cites were found in a registry or an index.
Protective effect of Moringa oleifera leaves ethanolic extract against thioacetamide-induced hepatotoxicity in rats via modulation of cellular antioxidant, apoptotic and inflammatory markers
Abstract
The current study was conducted to evaluate the ameliorative and protective potentials of Moringea oleifera leaves ethanolic extract (MOLE) against thioacetamide (TAA) toxicity. A total of 58 male albino rats were randomly assigned into six experimental groups. G1, rats received distilled water. G2, rats were injected with a single dose of TAA (200 mg/kg BW) i.p. G3, rats were given MOLE (300 mg/kg BW) orally for 26 days. G4, rats were injected TAA as in G2 and treated with MOLE as G3. G5, rats were kept for 26 days without treatment then on day 27 injected with TAA as in G2. G6, rats were given MOLE for 26 days then on day 27 injected with TAA. Phytochemical analysis of MOLE indicated the presence of kaempferol, kaempferol malonylglucoside, kaempferol hexoside, kaempferol -3-O-glucoside, kaempferol-3-O-acetyl-glucoside, cyanidin -3-O-hexoside, ellagic acid, quercetin, quercetin-3-O-glucoside, and apigenin glucoside. Intoxication of rats with TAA significantly elevated activities of serum AST, ALT, and ALP; concentrations of malondialdehyde, nitric oxide, and hepatic tissue protein expression of caspase 3 and COX2 with alteration of the histological structures of hepatic tissues, while it decreased serum levels of total protein, albumin, and hepatic tissue contents of reduced glutathione. Also, TAA intoxication resulted in 62.5% mortality in rats of G5. Treatment of TAA intoxicated rats (G4) with MOLE ameliorated the toxic effects of TAA on hepatic tissue structure and function. It decreased serum activities of AST, ALT, and ALP; enhanced hepatic GSH concentration; reduced pathological alterations and lipid peroxidation; and downregulated caspase 3 and COX2 proteins expression in hepatic tissue. In addition, MOLE protected rats of G6 from TAA-induced hepatic tissues injury and dysfunction, and increased survival rate of rats. In conclusion, MOLE had both ameliorating and protecting potentials against TAA-induced rats liver damage through regulation of antioxidant, anti-apoptotic, and inflammatory biomarkers. Graphical abstract.
Overview
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“This could help explain the higher flavonoid content observed in these regions, which may be linked to their antioxidant activity, as reflected by the TAC and DPPH results [32,33,34].”
From Comparative Analysis of Infusions and Ethanolic Extracts of Annona muricata Leaves from Colima, Mexico: Phytochemical Profile and Antioxidant Activity (2024) · cites this paper as [33]
In the citing paper
“Antioxidants play a crucial role in neutralizing free radicals, which are involved in the development of these conditions [33,57,67,68].”
From Comparative Analysis of Infusions and Ethanolic Extracts of Annona muricata Leaves from Colima, Mexico: Phytochemical Profile and Antioxidant Activity (2024) · cites this paper as [33]
Citations
Cites
1 retracted
RETRACTED: Role of Quercetin in Preventing Thioacetamide-Induced Liver Injury in Rats
de David, C., Rodrigues, G., Bona, S., Meurer, L., González‐Gallego, J., Tuñón, M. J., & Marroni, N. P. (2011) · Toxicologic Pathology · doi:10.1177/0192623311418680 · Cited by 116
RetractedAssay for lipid peroxides in animal tissues by thiobarbituric acid reaction
Ohkawa, H., Ohishi, N., & Yagi, K. (1979) · Analytical Biochemistry · doi:10.1016/0003-2697(79)90738-3 · Cited by 29,756
DETERMINATION OF SERUM PROTEINS BY MEANS OF THE BIURET REACTION
Gornall, A. G., Bardawill, C. J., & David, M. M. (1949) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)57021-6 · Cited by 16,164
Reitman, S., & Frankel, S. (1957) · American Journal of Clinical Pathology · doi:10.1093/ajcp/28.1.56 · Cited by 10,015
Improved method for the determination of blood glutathione.
Beutler, E., Duron, O., & KELLY, B. M. (1963) · PubMed · Cited by 6,932
The Merck Index: An Encyclopedia of Chemicals, Drugs, and Biologicals
(1990) · Annals of Internal Medicine · doi:10.7326/0003-4819-113-6-487_2 · Cited by 4,508
Albumin standards and the measurement of serum albumin with bromcresol green
Doumas, B. T., Watson, W. A., & Biggs, H. G. (1971) · Clinica Chimica Acta · doi:10.1016/0009-8981(71)90365-2 · Cited by 3,103
A RAPID AND PRECISE METHOD FOR THE DETERMINATION OF UREA
Fawcett, J. K., & Scott, J. E. (1960) · Journal of Clinical Pathology · doi:10.1136/jcp.13.2.156 · Cited by 2,845
Albumin standards and the measurement of serum albumin with bromcresol green
Doumas, B. T., Watson, W. A., & Biggs, H. G. (1997) · Clinica Chimica Acta · doi:10.1016/s0009-8981(96)06447-9 · Cited by 2,774
Bcl-2 and the regulation of programmed cell death
Reed, J. (1994) · The Journal of Cell Biology · doi:10.1083/jcb.124.1.1 · Cited by 2,287
Research works printed but not linked: 10
- Abalaka ME, Olonitola OS, Onaolapo JA, Inabo HI (2009) Evaluation of acute toxicity of Momordica charantia extract, using wistar rats to determine safety evels and usefulness of the plant in ethnochemotherapy. Int J Appl Sci 3:1–6
- Alain A, Akpovi CD, Sègbo J, Senou M, Anago E, Ahoyo TA et al (2016) Attenuation effect of Moringa oleifera leaves powder on blood biochemical disturbance induced in lead-exposed rats. Int Res J Biol Sci 5:14–21
- El-bakry K, Toson EL, Serag M, Aboser M (2016) Hepatoprotective effect of Moringa Oleifera leaves extract against carbon tetrachloride-induced liver damage in rats. World J Pharm Pharm Sci 5:76–89
- Hussein S, Elhadary A, Elgzar Y (2014) Biochemical study on the protective effect of curcumin on thioacetamide induced hepatotoxicity in rats. BVMJ 27:175–185
- Khawar MB, Abbasi MH et al (2016) Punjab Univ. Alterations in proteins and transaminases activity induced by thioacetamide in albino rats. J Zool 31:269–276
- Mansour HH, Abd El Azeem MG, Ismael NE (2014) Protective effect of Moringa oleifera on γ- radiation-induced hepatotoxicity and nephrotoxicity in rats. Am J Phytomed Clin Ther 2:495–508
- Minnady M, Paulraj-Dominic S, Thomas S, Subramanian S (2010) Therapeutic role of edible mushroom Pleurotus florida on thioacetamide induced hepatotoxicity in rats. Int J Curr Res 5:041–046
- Murray RL (1984) Creatinine. Kaplan A. Clin. Chem. The C. V. Mosby co. St. Louis. Toronto. Princeton, 1261-1266 and 418
- Roloff AH, Weisgerber H, Lang U, Stimm B, Schutt P (2009) Enzyklopdie der Holzgewchse. Handbuch und Atlas der Dendrologie 43:1–8
- Ujah OF, Ujah IR, Johnson JT, Ekam VS (2013) Effect of leaf extract of Moringa oleifera on carbon tetrachloride (CCl4) induced hepatotoxicity. J Nat Prod Plant Resour 3:15–22
Cited by
Sorted by citations
Hernandez-Fuentes, G. A., Delgado-Enciso, O. G., Larios-Cedeño, E. G., Sánchez-Galindo, J. M., Ceballos-Magaña, S. G., Pineda-Urbina, K., Alcalá-Pérez, M. A., Magaña-Vergara, N. E., Delgado-Enciso, J., Díaz-Llerenas, U., et al. (2024) · Life · doi:10.3390/life14121702 · Cited by 15
Metadata
- Authors
- Mousa, A. A., El-Gansh, H. A. I., Eldaim, M. A. A., Mohamed, M. A. E.-G., Morsi, A. H., & El Sabagh, H. S.
- Affiliations
- University of Sadat City (EG)
- Menoufia University (EG)
- Cairo University (EG)
- Corresponding author
- Eldaim, Mabrouk Attia Abd
- Venue
- Environmental Science and Pollution Research
- Published
- 2019-10-15
- Volume
- 26
- Issue
- 31
- Pages
- 32488-32504
- ISSN
- 0944-1344 (ISSN-L)
- 1614-7499
- OpenAlex
- W2980361925
- Cited by
- 70
- References
- 86
- Citations by year
- 2026: 8 · 2025: 10 · 2024: 10 · 2023: 12 · 2022: 12 · 2021: 10 · 2020: 8
- FWCI
- 5.53
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Environmental Science and Pollution Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Moringa oleifera research and applications
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
- Health Sciences › Medicine › Complementary and alternative medicine › Nigella sativa pharmacological applications
- Keywords
- Moringa oleifera, thioacetamide, hepatotoxicity, hepatoprotection, antioxidant defense, apoptosis, inflammation, caspase-3, cyclooxygenase-2, lipid peroxidation, oxidative stress, glutathione, kaempferol, quercetin, phytochemicals, liver enzymes, survival rate
- MeSH terms
- Animals, Antioxidants, Glucosides, Glutathione, Liver, Male, Malondialdehyde, Plant Extracts, Quercetin, Thioacetamide, Lipid Peroxidation, Biomarkers, Rats, Wistar, Plant Leaves, Protective Agents, Moringa oleifera, Rats, Chemical and Drug Induced Liver Injury
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Protective effect of Moringa oleifera leaves ethanolic extract against thioacetamide-induced hepatotoxicity in rats via modulation of cellular antioxidant, apoptotic and inflammatory markers. https://app.cochat.ai/papers/10.1007/s11356-019-06368-4
- The paper
- Mousa, A. A., El-Gansh, H. A. I., Eldaim, M. A. A., Mohamed, M. A. E.-G., Morsi, A. H., & El Sabagh, H. S. (2019). Protective effect of Moringa oleifera leaves ethanolic extract against thioacetamide-induced hepatotoxicity in rats via modulation of cellular antioxidant, apoptotic and inflammatory markers. Environmental Science and Pollution Research. https://doi.org/10.1007/s11356-019-06368-4
