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Cited by 30Abdominal Surgery and ComplicationsSepsis Diagnosis and TreatmentSurgical site infection prevention

Bacterial translocation and change in intestinal permeability in patients after abdominal surgery

Qiao, Zhi, Li, Zhanliang, Li, Jiye, Lu, Lianrong, Lv, Yi, Li, Junyou·2009·Journal of Huazhong University of Science and Technology [Medical Sciences]
DOI 10.1007/s11596-009-0419-3
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Abstract

The purpose of this study was to investigate bacterial translocation and change in intestinal permeability in patients after abdominal surgery. Sixty-three patients undergoing elective abdominal surgery were enrolled in the study. Blood samples were collected prior to operation and 2, 24, 48 h after surgery for bacterial culture, microbial DNA extraction, plasma D-lactate and endotoxin measurement. PCR analysis was performed after DNA extraction, with beta-lactosidase gene of E. coli and 16S rRNA gene as target genes. All patients were observed for a period of 30 days for infectious complications. Our results showed that no bacterial DNA was detected before surgery, but after operation it was found in 12 patients (19.0%). Bacterial DNA was detected in 41.7% (10/24) of SIRS patients and 5.1% (2/39) of non-SIRS patients (P<0.01). About 83.3% of PCR-positive patients developed systemic inflammatory response syndrome (SIRS), but only 27.5% of PCR-negative patients did so (P<0.01). Two thirds of PCR-positive patients developed infectious complications, while none of PCR-negative patients did (P<0.01). The blood culture was positive only in 3 patients (4.8%), who were all PCR-positive. E. coli DNA was found in 66.7% of the PCR-positive patients. The plasma levels of D-lactate and endotoxin were elevated significantly 2, 24 and 48 h after operation in PCR-positive patients, with a significant positive correlation found between them (r=0.91, P<0.01). It is concluded that increased intestinal permeability was closely related with bacterial translocation. Intestinal bacterial translocation (most commonly E. coli) might occur at early stage (2 h) after abdominal surgery. Postoperative SIRS and infection might bear a close relationship with bacterial translocation.

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  • Elevation of D-lactate in plasma may occur after abdominal surgery as a result of increased intestinal permeability and bacterial translocation across the intestinal mucosal barrier.

    “Elevation of D-lactate in plasma may also occur after other types of abdominal surgery, as a result of increased intestinal permeability and bacterial translocation across the intestinal mucosal barrier.62”

    From The Gut Microbiome and the Brain (2014) · cites this paper as [62]

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Authors
Qiao, Z., Li, Z., Li, J., Lu, L., Lv, Y., & Li, J.
Affiliations
  • Chinese PLA General Hospital (CN)
  • First Affiliated Hospital of Chinese PLA General Hospital (CN)
Corresponding author
Qiao, Zhi
ORCID
Venue
Journal of Huazhong University of Science and Technology [Medical Sciences]
Publisher
Springer Science and Business Media LLC
Published
2009-08
Volume
29
Issue
4
Pages
486-491
ISSN
  • 1672-0733 (ISSN-L)
  • 1993-1352
DOI
10.1007/s11596-009-0419-3
OpenAlex
W2083574710
Cited by
30
References
32
Citations by year
2025: 3 · 2024: 2 · 2023: 2 · 2021: 4 · 2020: 1 · 2019: 3 · 2018: 1 · 2017: 2 · 2016: 1 · 2015: 2 · 2014: 1 · 2013: 4
FWCI
1.12
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Journal of Huazhong University of Science and Technology [Medical Sciences] (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Abdominal Surgery and Complications
  • Health Sciences › Medicine › Epidemiology › Sepsis Diagnosis and Treatment
  • Health Sciences › Medicine › Surgery › Surgical site infection prevention
Keywords
bacterial translocation, intestinal permeability, abdominal surgery, systemic inflammatory response syndrome, postoperative infection, Escherichia coli, D-lactate, endotoxin, bacterial DNA detection, 16S rRNA gene
MeSH terms
Adult, Aged, Cell Membrane Permeability, Cholecystectomy, DNA, Bacterial, Escherichia coli, Female, Gastrectomy, Humans, Intestinal Mucosa, Male, Middle Aged, Postoperative Complications, Bacterial Translocation

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