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Cited by 8Genetic diversity and population structureIdentification and Quantification in FoodMolecular Biology Techniques and Applications

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Molecular detection of cell line cross-contaminations using amplified fragment length polymorphism DNA fingerprinting technology

Milanesi, E., Ajmone-Marsan, P., Bignotti, E., Losio, M. N., Bernardi, J., Chegdani, F., Soncini, M., Ferrari, M.·2003·In Vitro Cellular & Developmental Biology - Animal
DOI 10.1007/s11626-003-0006-z
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Abstract

We have tested amplified fragment length polymorphism (AFLP) technology, in comparison with isoenzyme analysis, for the simultaneous detection of inter- and intraspecific cell line cross-contaminations (CCCs) in the cell line collection held at the Istituto Zooprofilattico della Lombardia e dell'Emilia Romagna. Isoenzyme analysis identified four cases of interspecific CCCs. In a single experiment, AFLP was able to identify the species of origin of all cell lines for which a reference genomic deoxyribonucleic acid was available and to detect five interspecific contaminations. Four CCCs confirmed data on isoenzymes, whereas the fifth CCC was detected in a species for which isoenzyme analysis was noninformative. In addition, AFLP was able to identify the putative source of the contaminations detected. The utility of the technology in the detection of intraspecific cell line contaminations depends on the number of cell lines that have to be distinguished in a specific species and on the availability of highly informative fingerprinting systems. In mice, a single AFLP primer pair produced 16 polymorphisms and distinguished all the 15 strains of mouse cell lines analyzed. In humans, 18 AFLPs identified 83 different profiles in the 159 cell lines analyzed. Amplified fragment length polymorphism can conveniently be applied for cell line fingerprinting in species for which hypervariable markers are not available. In species for which a highly informative multiplex of microsatellite ma…

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Metadata

Authors
Milanesi, E., Ajmone-Marsan, P., Bignotti, E., Losio, M. N., Bernardi, J., Chegdani, F., Soncini, M., & Ferrari, M.
Affiliations
  • Università Cattolica del Sacro Cuore (IT)
  • Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna "Bruno Ubertini" (IT)
ORCID
Venue
In Vitro Cellular & Developmental Biology - Animal
Publisher
Springer Science and Business Media LLC
Published
2003-03
Volume
39
Issue
3-4
Pages
124-130
ISSN
  • 1071-2690 (ISSN-L)
  • 1543-706X
DOI
10.1007/s11626-003-0006-z
OpenAlex
W2061561173
Cited by
8
References
2
Citations by year
2024: 1 · 2017: 1 · 2013: 1
FWCI
0.18
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • In Vitro Cellular & Developmental Biology - Animal (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Università degli Studi della Tuscia
  • Universiteit Utrecht
  • Centre National de la Recherche Scientifique
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic diversity and population structure
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Identification and Quantification in Food
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
Keywords
cell line cross-contamination, AFLP markers, DNA fingerprinting, isozyme analysis, cell line authentication, mouse cell lines, human cell lines, contamination source identification
MeSH terms
Animals, Cell Line, Gene Amplification, Humans, Isoenzymes, Polymorphism, Genetic, Polymorphism, Restriction Fragment Length, DNA Fingerprinting, Cell Culture Techniques, Gene Expression Profiling, Mice

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