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Cited by 28Congenital heart defects researchMicroRNA in disease regulationCardiac Ischemia and Reperfusion

Upregulation of miRNA-23a-3p rescues high glucose-induced cell apoptosis and proliferation inhibition in cardiomyocytes

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Wu, Fang, Wang, Feng, Yang, Qian, Zhang, Yawen, Cai, Ke, Liu, Lian, Li, Shuchun, Zheng, YuanZheng +6 more·2020·In Vitro Cellular & Developmental Biology - Animal
DOI 10.1007/s11626-020-00518-6PMC PMC7723946
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Abstract

Maternal hyperglycemia potentially inhibits the development of the fetal heart by suppressing cardiomyocyte proliferation and promoting apoptosis. Different studies have indicated that miRNAs are key regulators of cardiomyocyte proliferation, differentiation, and apoptosis and play a protective role in a variety of cardiovascular diseases. However, the biological function of miRNA-23a in hyperglycemia-related cardiomyocyte injury is not fully understood. The present study investigated the effect of miRNA-23a-3p on cell proliferation and apoptosis in a myocardial injury model induced by high glucose. H9c2 cardiomyocytes were exposed to high glucose to establish an in vitro myocardial injury model and then transfected with miRNA-23a-3p mimics. After miRNA-23a-3p transfection, lens-free microscopy was used to dynamically monitor cell numbers and confluence and calculate the cell cycle duration. CCK-8 and EdU incorporation assays were performed to detect cell proliferation. Flow cytometry was used to measured cell apoptosis. Upregulation of miRNA-23a-3p significantly alleviated high glucose-induced cell apoptosis and cell proliferation inhibition (p < 0.01 and p < 0.0001, respectively). The cell cycle of the miRNA-23a-3p mimics group was significantly shorter than that of the negative control group (p < 0.01). The expression of cell cycle–activating and apoptosis inhibition-associated factors Ccna2, Ccne1, and Bcl-2 was downregulated by high glucose and upregulated by miRNA-23a-3p overexpression in high glucose-injured H9c2 cells. miRNA-23a-3p mimics transfection before high glucose treatment had a significantly greater benefit than transfection after high glucose treatment (p < 0.0001), and the rescue effect of miRNA-23a-3p increased as the concentration increased. This study suggests that miRNA-23a-3p exerted a dose- and time-dependent protective effect on high glucose-induced H9c2 cardiomyocyte injury.

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  • miRNA-23a-3p has a dose-dependent and time-dependent protective effect against high glucose-induced H9c2 cardiomyocyte injury.

    “It was also discovered that miRNA-23a-3p has a dose-dependent and time-dependent protective effect against high glucose-induced H9c2 cardiomyocyte injury [59].”

    From Recent advances of miR-23 in human diseases and growth development (2025) · cites this paper as [59]

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Authors
Wu, F., Wang, F., Yang, Q., Zhang, Y., Cai, K., Liu, L., Li, S., Zheng, Y., Zhang, J., Gui, Y., Wang, Y., Wang, X., Gui, Y., & Li, Q.
Affiliations
  • Shanghai Jiao Tong University (CN)
  • Fudan University (CN)
  • Children's Hospital of Fudan University (CN)
  • Shanghai University of Traditional Chinese Medicine (CN)
Corresponding author
  • Gui, Yiting
  • Gui, Yonghao
  • Li, Qiang
ORCID
Venue
In Vitro Cellular & Developmental Biology - Animal
Publisher
Springer Science and Business Media LLC
Published
2020-11-16
Volume
56
Issue
10
Pages
866-877
ISSN
  • 1071-2690 (ISSN-L)
  • 1543-706X
DOI
10.1007/s11626-020-00518-6
PMC
PMC7723946
OpenAlex
W3105762711
Cited by
28
References
2
Citations by year
2026: 2 · 2025: 4 · 2024: 3 · 2023: 3 · 2022: 11 · 2021: 5
FWCI
1.54
Type
journal article
Language
en
Open access
Licence
Locations
  • In Vitro Cellular & Developmental Biology - Animal (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (81470442, 81873481, 2016YFC1000500, 81771632)
  • National Key Research and Development Program of China (2016YFC1000500)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Congenital heart defects research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › MicroRNA in disease regulation
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Cardiac Ischemia and Reperfusion
Keywords
high glucose, cardiomyocyte apoptosis, cardiomyocyte proliferation, myocardial injury, maternal hyperglycemia, fetal heart development, cell cycle, cyclin A2, cyclin E1, Bcl-2, H9c2 cells
MeSH terms
Animals, Cell Line, Glucose, Up-Regulation, Apoptosis, Myocytes, Cardiac, MicroRNAs, Cell Proliferation, Rats

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