Improved production of antioxidant-phenolic compounds and certain fungal phenolic-associated enzymes under solid-state fermentation of chia seeds with Trichoderma reesei: Response surface methodology-based optimization
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Abstract Chia seeds (CS) are becoming increasingly consumed due to their great nutritional and therapeutic properties. In this study, solid-state fermentation (SSF) of CS by Trichoderma reesei was employed to maximize the production of the antioxidant-phenolic compounds and some fungal phenolic-associated enzymes (α-amylase, xylanase, β-glucosidase, polygalacturonase, and phenylalanine ammonia-lyase). The SSF-conditions were statistically optimized using response surface methodology (RSM). In the statistical model, four variables were analyzed at two levels. According to RSM, the adjusted R 2 (< 0.9) is reasonably consistent with the predicted R 2 (< 0.9), indicating that the statistical model is valid. The optimal conditions for maximum production of both phenolic compounds and fungal phenolic-associated enzymes were found to be 28 °C, pH 7.0, 20% moisture, and 7-day fermentation. The total phenolic content of fermented CS (FCS) increased 23 folds and total antioxidant activity was enhanced by 113- and 150-fold using DPPH and ABTS methods, respectively. Three new phenolics (kaempferol, apigenin, and p -coumaric) were recognized in FCS using HPLC analysis. The activities of all the extracted phenolic-associated enzymes showed strong correlations with the phenolic content of FCS. Against some human-pathogenic bacteria, FCS extract displayed considerably better antibacterial activity than UFCS extract. Finally, the phenolic-rich-FCS can be employed as a dietary supplement as well as an antibacterial agent. Furthermore, T. reesei has produced considerable quantities of industrially valuable enzymes.
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Flavonoid and phenolic contents of chia sprouts are significantly increased up to 6.4- and 11.5-fold on germination compared with dry seeds, respectively.
“The flavonoid and phenolic contents of chia sprouts are significantly increased up to 6.4- and 11.5-fold on germination with dry seeds, respectively [156].”
From Nutritional and Functional New Perspectives and Potential Health Benefits of Quinoa and Chia Seeds (2023) · cites this paper as [156]
Under similar conditions, chia sprout antioxidant activity is enhanced up to 29-fold.
“Under similar conditions, the sprout’s antioxidant activity enhanced up to 29-fold.”
From Nutritional and Functional New Perspectives and Potential Health Benefits of Quinoa and Chia Seeds (2023) · cites this paper as [156]
Chia sprout bioactivity shows potent antimicrobial activity towards enteric bacterial pathogens such as Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and S. aureus.
“The sprouts bioactivity showed potent antimicrobial activity towards enteric bacterial pathogens such as Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, and S. aureus [156].”
From Nutritional and Functional New Perspectives and Potential Health Benefits of Quinoa and Chia Seeds (2023) · cites this paper as [156]
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Microbial and enzymatic methods for phenolic production
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Phenolic profile, antioxidant and antibacterial activities of Balanites aegyptiaca fruit peel
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Self-citation
- Authors
- Abdel-Aty, A. M., Barakat, A. Z., Bassuiny, R. I., & Mohamed, S. A.
- Affiliations
- National Research Centre (EG)
- Corresponding author
- Mohamed, Saleh A.
- Venue
- Journal of Food Measurement and Characterization
- Published
- 2022-06-06
- Volume
- 16
- Issue
- 5
- Pages
- 3488-3500
- ISSN
- 2193-4126 (ISSN-L)
- 2193-4134
- OpenAlex
- W4281855346
- Cited by
- 50
- References
- 50
- Citations by year
- 2026: 10 · 2025: 20 · 2024: 11 · 2023: 8 · 2022: 1
- FWCI
- 5.23
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Journal of Food Measurement & Characterization (publishedVersion, cc-by)
- Greater South Information System
- Funders
- National Research Centre (12020110)
- Egyptian National Scientific and Technical Information Network Enstinet
- Topics
- Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
- Life Sciences › Agricultural and Biological Sciences › Food Science › Seed and Plant Biochemistry
- Life Sciences › Agricultural and Biological Sciences › Food Science › Food Quality and Safety Studies
- Keywords
- Salvia hispanica, Trichoderma reesei, solid-state fermentation, phenolic compounds, antioxidant activity, phenylalanine ammonia-lyase, xylanase, β-glucosidase, polygalacturonase, alpha-amylase, kaempferol, apigenin, p-coumaric acid, antibacterial activity, dietary supplements, enzyme production
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