Cited by 51Phytochemicals and Antioxidant ActivitiesSeed and Plant BiochemistryFood Quality and Safety Studies

Improved production of antioxidant-phenolic compounds and certain fungal phenolic-associated enzymes under solid-state fermentation of chia seeds with Trichoderma reesei: Response surface methodology-based optimization

Open access
Abdel-Aty, Azza M., Barakat, Amal Z., Bassuiny, Roqaya I., Mohamed, Saleh A.·2022·Journal of Food Measurement and Characterization

Abstract

Abstract Chia seeds (CS) are becoming increasingly consumed due to their great nutritional and therapeutic properties. In this study, solid-state fermentation (SSF) of CS by Trichoderma reesei was employed to maximize the production of the antioxidant-phenolic compounds and some fungal phenolic-associated enzymes (α-amylase, xylanase, β-glucosidase, polygalacturonase, and phenylalanine ammonia-lyase). The SSF-conditions were statistically optimized using response surface methodology (RSM). In the statistical model, four variables were analyzed at two levels. According to RSM, the adjusted R 2 (< 0.9) is reasonably consistent with the predicted R 2 (< 0.9), indicating that the statistical model is valid. The optimal conditions for maximum production of both phenolic compounds and fungal phenolic-associated enzymes were found to be 28 °C, pH 7.0, 20% moisture, and 7-day fermentation. The total phenolic content of fermented CS (FCS) increased 23 folds and total antioxidant activity was enhanced by 113- and 150-fold using DPPH and ABTS methods, respectively. Three new phenolics (kaempferol, apigenin, and p -coumaric) were recognized in FCS using HPLC analysis. The activities of all the extracted phenolic-associated enzymes showed strong correlations with the phenolic content of FCS. Against some human-pathogenic bacteria, FCS extract displayed considerably better antibacterial activity than UFCS extract. Finally, the phenolic-rich-FCS can be employed as a dietary supplement as well as an antibacterial agent. Furthermore, T. reesei has produced considerable quantities of industrially valuable enzymes.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1007/s11694-022-01447-6

Cites

50 of 50 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Cites

Flagged first, then by citations

Research works printed but not linked: 3

  1. Chia. (Salvia hispanica L.): A review. J. Food Sci. Technol. 53, 1750-1758 (2016)
  2. A.W. Bauer, W.M. Kirby, J.C. Sherris, M. Turk, J. Am, Antibiotic susceptibility testing by a standardized single disk method. Clin. Pathol. 44, 493 (1996)
  3. National Committee for Clinical Laboratory Standards, Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically, Approved Standard M7-A7, vol. 26 (Clinical and Laboratory Standards Institute, Wayne, PA, 2009)

Cited by

Sorted by citations

Authors
Abdel-Aty, A. M., Barakat, A. Z., Bassuiny, R. I., & Mohamed, S. A.
Affiliations
National Research Centre (EG)
Corresponding author
Mohamed, Saleh A.
Venue
Journal of Food Measurement and Characterization
Published
2022-06-06
Volume
16
Issue
5
Pages
3488-3500
ISSN
  • 2193-4126 (ISSN-L)
  • 2193-4134
OpenAlex
W4281855346
Cited by
50
References
50
Citations by year
2026: 10 · 2025: 20 · 2024: 11 · 2023: 8 · 2022: 1
FWCI
5.23
Type
journal article
Language
en
Open access
Locations
  • Journal of Food Measurement & Characterization (publishedVersion, cc-by)
  • Greater South Information System
Funders
  • National Research Centre (12020110)
  • Egyptian National Scientific and Technical Information Network Enstinet
Topics
  • Health Sciences › Medicine › Biochemistry › Phytochemicals and Antioxidant Activities
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Seed and Plant Biochemistry
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Food Quality and Safety Studies
Keywords
Salvia hispanica, Trichoderma reesei, solid-state fermentation, phenolic compounds, antioxidant activity, phenylalanine ammonia-lyase, xylanase, β-glucosidase, polygalacturonase, alpha-amylase, kaempferol, apigenin, p-coumaric acid, antibacterial activity, dietary supplements, enzyme production

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review