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Purification and characterization of antioxidative peptides from protein hydrolysate of lecithin‐free egg yolk
Abstract
The protein extracted from lecithin‐free egg yolk, normally discarded by lecithin processing plants, was hydrolyzed with the aid of Alcalase, a commercial enzyme. The hydrolysate was separated through a series of ultrafiltration membranes with molecular weight cutoffs of 10, 5, and 1 kDa; and three types of permeates including 10 K (permeate from 10 kDa), 5 K (permeate from 5 kDa), and 1 K (permeate from 1 kDa) were obtained. The antioxidative efficacy of hydrolysates so obtained was investigated and compared with α‐tocopherol. Furthermore, two different peptides showing strong antioxidative activity were isolated from the hydrolysates by using consecutive chromatographic methods including ion exchange chromatography on a SP‐Sephadex C‐25 column, gel filtration on a Sephadex G‐25 column, and high‐performance liquid chromatography on an octadecylsilane column. The purity of the peptides was identified using capillary electrophoresis. The isolated peptides were composed of 10 and 15 amino acid residues, and both contained a leucine residue at their N‐terminal positions.
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Cited by
Wijesekara, I., Pangestuti, R., & Kim, S.-K. (2011) · Carbohydrate Polymers · doi:10.1016/j.carbpol.2010.10.062 · Cited by 992
The possible roles of food-derived bioactive peptides in reducing the risk of cardiovascular disease
Erdmann, K., Cheung, B. W. Y., & Schröder, H. (2008) · The Journal of Nutritional Biochemistry · doi:10.1016/j.jnutbio.2007.11.010 · Cited by 717
Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis
Dávalos, A., Miguel, M., Bartolomé, B., & López-Fandiño, R. (2004) · Journal of Food Protection · doi:10.4315/0362-028x-67.9.1939 · Cited by 495
Marine Bioactive Compounds and Their Health Benefits: A Review
Hamed, I., Özogul, F., Özogul, Y., & Regenstein, J. M. (2015) · Comprehensive Reviews in Food Science and Food Safety · doi:10.1111/1541-4337.12136 · Cited by 444
Ngo, D.-H., Vo, T.-S., Ngo, D.-N., Wijesekara, I., & Kim, S.-K. (2012) · International Journal of Biological Macromolecules · doi:10.1016/j.ijbiomac.2012.06.001 · Cited by 301
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Metadata
- Authors
- Park, P., Jung, W., Nam, K., Shahidi, F., & Kim, S.
- Venue
- Journal of the American Oil Chemists' Society
- Publisher
- Wiley
- Published
- 2001-06
- Volume
- 78
- Issue
- 6
- Pages
- 651-656
- ISSN
- 0003-021X (ISSN-L)
- 1558-9331
- OpenAlex
- W1977390994
- Cited by
- 340
- References
- 0
- FWCI
- 1.41
- Type
- journal article
- Language
- en
- Open access
- closed
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- Park, P., Jung, W., Nam, K., Shahidi, F., & Kim, S. (2001). Purification and characterization of antioxidative peptides from protein hydrolysate of lecithin‐free egg yolk. Journal of the American Oil Chemists' Society. https://doi.org/10.1007/s11746-001-0321-0
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