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Purification and characterization of antioxidative peptides from protein hydrolysate of lecithin‐free egg yolk

Park, Pyo‐Jam, Jung, Won‐Kyo, Nam, Kyung‐Soo, Shahidi, F., Kim, Se‐Kwon·2001·Journal of the American Oil Chemists' Society

Abstract

The protein extracted from lecithin‐free egg yolk, normally discarded by lecithin processing plants, was hydrolyzed with the aid of Alcalase, a commercial enzyme. The hydrolysate was separated through a series of ultrafiltration membranes with molecular weight cutoffs of 10, 5, and 1 kDa; and three types of permeates including 10 K (permeate from 10 kDa), 5 K (permeate from 5 kDa), and 1 K (permeate from 1 kDa) were obtained. The antioxidative efficacy of hydrolysates so obtained was investigated and compared with α‐tocopherol. Furthermore, two different peptides showing strong antioxidative activity were isolated from the hydrolysates by using consecutive chromatographic methods including ion exchange chromatography on a SP‐Sephadex C‐25 column, gel filtration on a Sephadex G‐25 column, and high‐performance liquid chromatography on an octadecylsilane column. The purity of the peptides was identified using capillary electrophoresis. The isolated peptides were composed of 10 and 15 amino acid residues, and both contained a leucine residue at their N‐terminal positions.

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Metadata

Authors
Park, P., Jung, W., Nam, K., Shahidi, F., & Kim, S.
Venue
Journal of the American Oil Chemists' Society
Publisher
Wiley
Published
2001-06
Volume
78
Issue
6
Pages
651-656
ISSN
  • 0003-021X (ISSN-L)
  • 1558-9331
OpenAlex
W1977390994
Cited by
340
References
0
FWCI
1.41
Type
journal article
Language
en
Open access
closed

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CoChat. (2026). Citation check: Purification and characterization of antioxidative peptides from protein hydrolysate of lecithin‐free egg yolk. https://app.cochat.ai/papers/10.1007/s11746-001-0321-0
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Park, P., Jung, W., Nam, K., Shahidi, F., & Kim, S. (2001). Purification and characterization of antioxidative peptides from protein hydrolysate of lecithin‐free egg yolk. Journal of the American Oil Chemists' Society. https://doi.org/10.1007/s11746-001-0321-0

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