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Ligand Binding of PR-10 Proteins with a Particular Focus on the Bet v 1 Allergen Family

Aglas, Lorenz, Soh, Wai Tuck, Kraiem, Amin, Wenger, Mario, Brandstetter, Hans, Ferreira, Fatima·2020·Current Allergy and Asthma Reports
DOI 10.1007/s11882-020-00918-4PMC PMC7237532
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Abstract

Purpose of Review: Pathogenesis-related class 10 (PR-10) proteins are highly conserved plant proteins, which are induced in response to abiotic and biotic stress factors. To date, no unique biological function could be assigned to them. Rather a more general role of PR-10 in plant development and defense mechanisms has been proposed. In addition, some PR-10 proteins act as allergens by triggering allergic symptoms in sensitized individuals. Regardless of the diversity of reported activities, all PR-10 proteins share a common fold characterized by a solvent-accessible hydrophobic cavity, which serves as a binding site for a myriad of small-molecule ligands, mostly phytohormones and flavonoids. Recent Findings: Most of available data relate to the ligand binding activity of allergenic PR-10, particularly for those belonging to Bet v 1 family of allergens. Bet v 1 and its homologues were shown to bind flavonoids with high affinity, but the specificity appears to differ between homologues from different species. The flavonoid Q3O-(Glc)-Gal was shown to specifically bind to hazelnut Cor a 1 but not to Bet v 1. Similarly, Q3OS bound only to the major isoform Bet v 1.0101 and not to other closely related isoforms. In contrast, Bet v 1 and hazelnut Cor a 1 showed very similar binding behavior towards other flavonoids such as quercetin, genistein, apigenin, daidzein, and resveratrol. Summary: Recent research findings highlighted the importance of more precise knowledge of ligand binding for understanding the functional diversification of PR-10 proteins.

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Fig. 1 Overview on described functions associated with PR-10 ligand binding; PR-10, pathogenesis-related proteins class 10; IPR, intracellular pathogenesis-related proteins/classic PR-10 proteins; CSBP, cytokinin-specific binding proteins; MLP, major latex proteins; other PR-10-like, not yet classified proteins possessing a PR-10-like fold
Fig. 1 Overview on described functions associated with PR-10 ligand binding; PR-10, pathogenesis-related proteins class 10; IPR, intracellular pathogenesis-related proteins/classic PR-10 proteins; CSBP, cytokinin-specific binding proteins; MLP, major latex proteins; other PR-10-like, not yet classified proteins possessing a PR-10-like foldCC BY · PubMed Central

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Research works printed but not linked: 56

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Metadata

Authors
Aglas, L., Soh, W. T., Kraiem, A., Wenger, M., Brandstetter, H., & Ferreira, F.
Venue
Current Allergy and Asthma Reports
Publisher
Springer Science and Business Media LLC
Published
2020-05-19
Volume
20
Issue
7
ISSN
  • 1529-7322
  • 1534-6315
DOI
10.1007/s11882-020-00918-4
PMC
PMC7237532
Cited by
56
References
0
Language
en
Licence
Funders
  • Austrian Science Fund (P27589, P32189, W1213)
  • Salzburg priority program “Allergy-Cancer-BioNano Research Centre
  • Doctoral School Program “Biomolecules” of the University of Salzburg

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