The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.
Cellular GFP Toxicity and Immunogenicity: Potential Confounders in in Vivo Cell Tracking Experiments
Open accessAbstract
Green Fluorescent protein (GFP), used as a cellular tag, provides researchers with a valuable method of measuring gene expression and cell tracking. However, there is evidence to suggest that the immunogenicity and cytotoxicity of GFP potentially confounds the interpretation of in vivo experimental data. Studies have shown that GFP expression can deteriorate over time as GFP tagged cells are prone to death. Therefore, the cells that were originally marked with GFP do not survive and cannot be accurately traced over time. This review will present current evidence for the immunogenicity and cytotoxicity of GFP in in vivo studies by characterizing these responses.
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Cites
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Cited by
AAV cis -regulatory sequences are correlated with ocular toxicity
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Systemic and local immune responses to intraocular AAV vector administration in non-human primates
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“Other factors that can influence the immune responses are the AAV serotype used,(18) the virus dose injected,(19) whether the injection is re-administrated,(20) and the promoter(21,22) and transgene used.(23)”
Background
Metadata
- Authors
- Ansari, A. M., Ahmed, A. K., Matsangos, A. E., Lay, F., Born, L. J., Marti, G., Harmon, J. W., & Sun, Z.
- Venue
- Stem Cell Reviews and Reports
- Published
- 2016-07-19
- Volume
- 12
- Issue
- 5
- Pages
- 553-559
- ISSN
- 1550-8943 (ISSN-L)
- 1558-6804
- OpenAlex
- W2488203923
- Cited by
- 323
- References
- 54
- FWCI
- 13.95
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Ansari, A. M., Ahmed, A. K., Matsangos, A. E., Lay, F., Born, L. J., Marti, G., Harmon, J. W., & Sun, Z. (2016). Cellular GFP Toxicity and Immunogenicity: Potential Confounders in in Vivo Cell Tracking Experiments. Stem Cell Reviews and Reports. https://doi.org/10.1007/s12015-016-9670-8
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