Cited by 6,802Advanced Fluorescence Microscopy Techniquesbioluminescence and chemiluminescence researchMolecular Biology Techniques and Applications

Green fluorescent protein as a marker for gene expression

Chalfie, Martin, Tu, Yuan, Euskirchen, Ghia, Ward, William W., Prasher, Douglas C.·1994·Science

Abstract

A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.

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Crossref · 10.1126/science.8303295

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1 of 1 references verified0 citation errors · 0 not checkable

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Authors
Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W., & Prasher, D. C.
Affiliations
  • Columbia University (US)
  • Rutgers, The State University of New Jersey (US)
  • Woods Hole Oceanographic Institution (US)
Venue
Science
Published
1994-02-11
Volume
263
Issue
5148
Pages
802-805
ISSN
  • 0036-8075 (ISSN-L)
  • 1095-9203
OpenAlex
W2032579724
Cited by
6,802
References
1
Citations by year
2026: 90 · 2025: 127 · 2024: 112 · 2023: 131 · 2022: 143 · 2021: 152 · 2020: 179 · 2019: 169 · 2018: 185 · 2017: 180 · 2016: 184 · 2015: 203 · 2014: 210 · 2013: 209 · 2012: 242
FWCI
158.36
Type
journal article
Language
en
Open access
closed
Locations
  • Science (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institute of General Medical Sciences (GM31997)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › bioluminescence and chemiluminescence research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
Keywords
green fluorescent protein, Aequorea victoria, gene expression biomarkers, reporter genes, protein localization, Caenorhabditis elegans, Escherichia coli
MeSH terms
Animals, Base Sequence, Cell Division, Cell Separation, Escherichia coli, Fluorescence, Luminescent Proteins, Microscopy, Fluorescence, Molecular Sequence Data, Neurons, Oligodeoxyribonucleotides, Recombinant Proteins, Spectrometry, Fluorescence, Transformation, Genetic, Gene Expression, Caenorhabditis elegans, Green Fluorescent Proteins

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