Green fluorescent protein as a marker for gene expression
Abstract
A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.
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BacMam is a modified baculovirus produced using Sf9 insect cells and pseudotyped to infect mammalian cells.
“BacMam is a modified baculovirus produced using Sf9 insect cells and it is pseudotyped to infect mammalian cells [54,55,56].”
From Effects of Mangiferin on LPS-Induced Inflammation and SARS-CoV-2 Viral Adsorption in Human Lung Cells (2022) · cites this paper as [55]
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- Authors
- Chalfie, M., Tu, Y., Euskirchen, G., Ward, W. W., & Prasher, D. C.
- Affiliations
- Columbia University (US)
- Rutgers, The State University of New Jersey (US)
- Woods Hole Oceanographic Institution (US)
- ORCID
- Chalfie, Martin
- Venue
- Science
- Published
- 1994-02-11
- Volume
- 263
- Issue
- 5148
- Pages
- 802-805
- ISSN
- 0036-8075 (ISSN-L)
- 1095-9203
- OpenAlex
- W2032579724
- Cited by
- 6,802
- References
- 1
- Citations by year
- 2026: 90 · 2025: 127 · 2024: 112 · 2023: 131 · 2022: 143 · 2021: 152 · 2020: 179 · 2019: 169 · 2018: 185 · 2017: 180 · 2016: 184 · 2015: 203 · 2014: 210 · 2013: 209 · 2012: 242
- FWCI
- 158.36
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Science (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM31997)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biophysics › Advanced Fluorescence Microscopy Techniques
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › bioluminescence and chemiluminescence research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- green fluorescent protein, Aequorea victoria, gene expression biomarkers, reporter genes, protein localization, Caenorhabditis elegans, Escherichia coli
- MeSH terms
- Animals, Base Sequence, Cell Division, Cell Separation, Escherichia coli, Fluorescence, Luminescent Proteins, Microscopy, Fluorescence, Molecular Sequence Data, Neurons, Oligodeoxyribonucleotides, Recombinant Proteins, Spectrometry, Fluorescence, Transformation, Genetic, Gene Expression, Caenorhabditis elegans, Green Fluorescent Proteins
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