Human parenchymal and non-parenchymal liver cell isolation, culture and characterization
Abstract
Many reports describing parenchymal liver cell isolation have been published so far. However, recent evidence has clearly demonstrated that non-parenchymal liver cells play an important role in many pathophysiologies of the liver, such as drug-induced liver diseases, inflammation, and the development of liver fibrosis and cirrhosis. In this study, we present an overview of the current methods for isolating and characterizing parenchymal and non-parenchymal liver cells.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The liver is composed of non-parenchymal cells, which include hepatic stellate cells (HSCs), resident macrophages, endothelial cells (ECs), and cholangiocytes.
“The liver is also composed of non-parenchymal cells, which include hepatic stellate cells (HSCs), resident macrophages, endothelial cells (ECs), and cholangiocytes8,9.”
From Spatial transcriptomics of healthy and fibrotic human liver at single-cell resolution (2025) · cites this paper as [8]
Cites
Godoy, P., Hewitt, N. J., Albrecht, U., Andersen, M. E., Ansari, N., Bhattacharya, S., Bode, J. G., Bolleyn, J., Borner, C., Böttger, J., et al. (2013) · Archives of Toxicology · doi:10.1007/s00204-013-1078-5 · Cited by 1,280 · see citing works
Self-citation
Flagged first, then by citations
Cited by
Sorted by citations
Cell sources for <i>in vitro</i> human liver cell culture models
Zeilinger, K., Freyer, N., Damm, G., Seehofer, D., & Knöspel, F. (2016) · Experimental Biology and Medicine · doi:10.1177/1535370216657448 · Cited by 230 · see citing works
Self-citationSpatial transcriptomics of healthy and fibrotic human liver at single-cell resolution
Watson, B. R., Paul, B., Rahman, R. U., Amir-Zilberstein, L., Segerstolpe, Å., Epstein, E. T., Murphy, S., Geistlinger, L., Lee, T., Shih, A., et al. (2025) · Nature Communications · doi:10.1038/s41467-024-55325-4 · Cited by 123 · see citing works
Hepatic chemerin <scp>mRNA</scp> expression is reduced in human nonalcoholic steatohepatitis
Pohl, R., Haberl, E. M., Rein‐Fischboeck, L., Zimny, S., Neumann, M., Aslanidis, C., Schacherer, D., Krautbauer, S., Eisinger, K., Weiss, T. S., et al. (2017) · European Journal of Clinical Investigation · doi:10.1111/eci.12695 · Cited by 45 · see citing works
- Authors
- Damm, G., Pfeiffer, E., Burkhardt, B., Vermehren, J., Nüssler, A. K., & Weiss, T. S.
- Affiliations
- Charité – Universitätsmedizin Berlin (DE)
- BG Klinik Tübingen (DE)
- University Hospital Regensburg (DE)
- Venue
- Hepatology International
- Published
- 2013-10
- Volume
- 7
- Issue
- 4
- Pages
- 951-958
- ISSN
- 1936-0533 (ISSN-L)
- 1936-0541
- OpenAlex
- W2086726448
- Cited by
- 36
- References
- 1
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 2 · 2023: 4 · 2022: 4 · 2021: 2 · 2020: 1 · 2019: 1 · 2017: 5 · 2016: 7 · 2015: 3 · 2014: 3
- FWCI
- 2.69
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Hepatology International (publishedVersion)
- PubMed (publishedVersion)
- University of Regensburg Publication Server (University of Regensburg) (acceptedVersion)
- Funders
- Bundesministerium für Bildung und Forschung
- Topics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Health Sciences › Medicine › Surgery › Organ Transplantation Techniques and Outcomes
- Health Sciences › Medicine › Epidemiology › Liver Disease Diagnosis and Treatment
- Keywords
- liver cell isolation, non-parenchymal liver cells, liver cell culture, liver fibrosis, liver cirrhosis, drug-induced liver injury, liver inflammation, cell characterization
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
