Cell sources for in vitro human liver cell culture models

Zeilinger, Katrin; Freyer, Nora; Damm, Georg; Seehofer, Daniel; Knöspel, Fanny

doi:10.1177/1535370216657448

Cited by 230Drug-Induced Hepatotoxicity and ProtectionLiver physiology and pathology3D Printing in Biomedical Research

Cell sources for in vitro human liver cell culture models

Open access

Zeilinger, Katrin, Freyer, Nora, Damm, Georg, Seehofer, Daniel, Knöspel, Fanny·2016·Experimental Biology and Medicine

DOI 10.1177/1535370216657448PMC PMC4999620

Open this paper in CoChat — free account

Abstract

In vitro liver cell culture models are gaining increasing importance in pharmacological and toxicological research. The source of cells used is critical for the relevance and the predictive value of such models. Primary human hepatocytes (PHH) are currently considered to be the gold standard for hepatic in vitro culture models, since they directly reflect the specific metabolism and functionality of the human liver; however, the scarcity and difficult logistics of PHH have driven researchers to explore alternative cell sources, including liver cell lines and pluripotent stem cells. Liver cell lines generated from hepatomas or by genetic manipulation are widely used due to their good availability, but they are generally altered in certain metabolic functions. For the past few years, adult and pluripotent stem cells have been attracting increasing attention, due their ability to proliferate and to differentiate into hepatocyte-like cells in vitro. However, controlling the differentiation of these cells is still a challenge. This review gives an overview of the major human cell sources under investigation for in vitro liver cell culture models, including primary human liver cells, liver cell lines, and stem cells. The promises and challenges of different cell types are discussed with a focus on the complex 2D and 3D culture approaches under investigation for improving liver cell functionality in vitro. Finally, the specific application options of individual cell sources in pharmacological research or disease modeling are described.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1177/1535370216657448

Cites

158 of 158 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

The most commonly applied ECM components for hepatocyte cultures are collagen type I and Matrigel.
“The most commonly applied ECM components for hepatocyte cultures are collagen type I and Matrigel, a commercially available BM matrix extract from Engelbreth-Holm-Swarm (EHS) mouse sarcoma (Serna-Márquez et al., 2020[35]; Zeilinger et al., 2016[49]).”
From Optimization of extracellular matrix for primary human hepatocyte cultures using mixed collagen-matrigel matrices (2023) · cites this paper as Zeilinger et al., 2016[49]
The “2D and 3D culture models for PHH” section identifies rat-tail collagen and Matrigel as ECM components used in hepatocyte sandwich cultures. It does not establish that they are the most commonly applied components across hepatocyte cultures generally. (LLM, conf=0.82)
Experimental — accuracy not yet measured

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Induction of Pluripotent Stem Cells from Adult Human Fibroblasts by Defined Factors
Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., & Yamanaka, S. (2007) · Cell · doi:10.1016/j.cell.2007.11.019 · Cited by 20,221 · see citing works
Embryonic Stem Cell Lines Derived from Human Blastocysts
Thomson, J. A., Itskovitz‐Eldor, J., Shapiro, S. S., Waknitz, M. A., Swiergiel, J. J., Marshall, V. S., & Jones, J. M. (1998) · Science · doi:10.1126/science.282.5391.1145 · Cited by 15,947 · see citing works
Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells
Yu, J., Vodyanik, M. A., Smuga-Otto, K., Antosiewicz-Bourget, J., Frane, J. L., Tian, S., Nie, J., Jonsdottir, G. A., Ruotti, V., Stewart, R., et al. (2007) · Science · doi:10.1126/science.1151526 · Cited by 10,310 · see citing works
Vascularized and functional human liver from an iPSC-derived organ bud transplant
Takebe, T., Sekine, K., Enomura, M., Koike, H., Kimura, M., Ogaeri, T., Zhang, R.-R., Ueno, Y., Zheng, Y.-W., Koike, N., et al. (2013) · Nature · doi:10.1038/nature12271 · Cited by 2,170 · see citing works
Recent advances in 2D and 3D in vitro systems using primary hepatocytes, alternative hepatocyte sources and non-parenchymal liver cells and their use in investigating mechanisms of hepatotoxicity, cell signaling and ADME
Godoy, P., Hewitt, N. J., Albrecht, U., Andersen, M. E., Ansari, N., Bhattacharya, S., Bode, J. G., Bolleyn, J., Borner, C., Böttger, J., et al. (2013) · Archives of Toxicology · doi:10.1007/s00204-013-1078-5 · Cited by 1,280 · see citing works
Self-citation
Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells
Schwartz, R. E., Reyes, M., Koodie, L., Jiang, Y., Blackstad, M., Lund, T. C., Lenvik, T. R., Johnson, S., Hu, W., & Verfaillie, C. M. (2002) · Journal of Clinical Investigation · doi:10.1172/jci15182 · Cited by 1,030 · see citing works
Effect of cell–cell interactions in preservation of cellular phenotype: cocultivation of hepatocytes and nonparenchymal cells
Bhatia, S. N., Balis, U. J., Yarmush, M. L., & Toner, M. (1999) · The FASEB Journal · doi:10.1096/fasebj.13.14.1883 · Cited by 890 · see citing works
Multipotent adult progenitor cells from bone marrow differentiate into functional hepatocyte-like cells
Schwartz, R. E., Reyes, M., Koodie, L., Jiang, Y., Blackstad, M., Lund, T. C., Lenvik, T. R., Johnson, S., Hu, W., & Verfaillie, C. M. (2002) · Journal of Clinical Investigation · doi:10.1172/jci0215182 · Cited by 863 · see citing works
Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease
Bell, C. C., Hendriks, D. F. G., Moro, S. M. L., Ellis, E., Walsh, J., Renblom, A., Fredriksson Puigvert, L., Dankers, A. C. A., Jacobs, F., Snoeys, J., et al. (2016) · Scientific Reports · doi:10.1038/srep25187 · Cited by 739 · see citing works
The canals of hering and hepatic stem cells in humans
Theise, N. D., Saxena, R., Portmann, B. C., Thung, S. N., Yee, H. T., Chiriboga, L. A., Kumar, A., & Crawford, J. M. (1999) · Hepatology · doi:10.1002/hep.510300614 · Cited by 700 · see citing works
Characterization of primary human hepatocytes, HepG2 cells, and HepaRG cells at the mRNA level and CYP activity in response to inducers and their predictivity for the detection of human hepatotoxins
Gerets, H. H. J., Tilmant, K., Gerin, B., Chanteux, H., Depelchin, B. O., Dhalluin, S., & Atienzar, F. A. (2012) · Cell Biology and Toxicology · doi:10.1007/s10565-011-9208-4 · Cited by 698 · see citing works
The human hepatoma HepaRG cells: A highly differentiated model for studies of liver metabolism and toxicity of xenobiotics
Guillouzo, A., Corlu, A., Aninat, C., Glaise, D., Morel, F., & Guguen‐Guillouzo, C. (2006) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2006.12.003 · Cited by 675 · see citing works
A liver-on-a-chip platform with bioprinted hepatic spheroids
Bhise, N. S., Manoharan, V., Massa, S., Tamayol, A., Ghaderi, M., Miscuglio, M., Lang, Q., Shrike Zhang, Y., Shin, S. R., Calzone, G., et al. (2016) · Biofabrication · doi:10.1088/1758-5090/8/1/014101 · Cited by 615 · see citing works
Modeling inherited metabolic disorders of the liver using human induced pluripotent stem cells
Rashid, S. T., Corbineau, S., Hannan, N. R. F., Marciniak, S. J., Miranda, E., Alexander, G., Huang‐Doran, I., Griffin, J. L., Ährlund‐Richter, L., Skepper, J. N., et al. (2010) · Journal of Clinical Investigation · doi:10.1172/jci43122 · Cited by 598 · see citing works
Directed assembly of cell-laden microgels for fabrication of 3D tissue constructs
Du, Y., Lo, E., Ali, S., & Khademhosseini, A. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0801866105 · Cited by 594 · see citing works
Human hepatic stem cells from fetal and postnatal donors
Schmelzer, E., Zhang, L., Bruce, A., Wauthier, E., Ludlow, J. W., Yao, H.-L., Moss, N., Melhem, A., McClelland, R., Turner, W. S., et al. (2007) · The Journal of Experimental Medicine · doi:10.1084/jem.20061603 · Cited by 585 · see citing works
Generation of Functional Hepatocytes From Human Embryonic Stem Cells Under Chemically Defined Conditions That Recapitulate Liver Development
Touboul, T., Hannan, N. R. F., Corbineau, S., Martinez, A., Martinet, C., Branchereau, S., Goulinet, S., Strick‐Marchand, H., Pedersen, R. A., Santo, J. P. D., et al. (2009) · Hepatology · doi:10.1002/hep.23506 · Cited by 499 · see citing works
Highly efficient differentiation of hESCs to functional hepatic endoderm requires ActivinA and Wnt3a signaling
Hay, D. C., Fletcher, J. M., Payne, C. M., Terrace, J. D., Gallagher, R., Snoeys, J., Black, J. R., Wojtacha, D., Samuel, K., Hannoun, Z., et al. (2008) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0806522105 · Cited by 434 · see citing works
Efficient endoderm induction from human pluripotent stem cells by logically directing signals controlling lineage bifurcations
Loh, K. M., Ang, L. T., Zhang, J., Kumar, V., Ang, J., Auyeong, J. Q., Lee, K. L., Choo, S. H., Lim, C. Y. Y., Nichane, M., et al. (2014) · Cell Stem Cell · doi:10.1016/j.stem.2013.12.007 · Cited by 418 · see citing works
Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation
Sampaziotis, F., de Brito, M. C., Madrigal, P., Bertero, A., Saeb‐Parsy, K., Soares, F. A. C., Schrumpf, E., Melum, E., Karlsen, T. H., Bradley, J. A., et al. (2015) · Nature Biotechnology · doi:10.1038/nbt.3275 · Cited by 398 · see citing works
Phenotypic and functional analyses show stem cell-derived hepatocyte-like cells better mimic fetal rather than adult hepatocytes
Baxter, M. A., Withey, S., Harrison, S. P., Segeritz, C., Zhang, F., Atkinson-Dell, R., Rowe, C., Gerrard, D. T., Sison-Young, R. L. C., Jenkins, R., et al. (2014) · Journal of Hepatology · doi:10.1016/j.jhep.2014.10.016 · Cited by 353 · see citing works
The Phenotypes of Pluripotent Human Hepatic Progenitors
Schmelzer, E., Wauthier, E., & Reid, L. M. (2006) · Stem Cells · doi:10.1634/stemcells.2006-0036 · Cited by 333 · see citing works
Production of hepatocyte-like cells from human pluripotent stem cells
Hannan, N. R. F., Segeritz, C., Touboul, T., & Vallier, L. (2013) · Nature Protocols · doi:10.1038/nprot.2012.153 · Cited by 327 · see citing works
Multi-cell type human liver microtissues for hepatotoxicity testing
Messner, S., Agarkova, I., Moritz, W., & Kelm, J. M. (2013) · Archives of Toxicology · doi:10.1007/s00204-012-0968-2 · Cited by 319 · see citing works
DNA Methylation Dynamics in Human Induced Pluripotent Stem Cells over Time
Nishino, K., Toyoda, M., Yamazaki‐Inoue, M., Fukawatase, Y., Chikazawa, E., Sakaguchi, H., Akutsu, H., & Umezawa, A. (2011) · PLoS Genetics · doi:10.1371/journal.pgen.1002085 · Cited by 312 · see citing works
Molecular Mechanisms Underlying the Dedifferentiation Process of Isolated Hepatocytes and Their Cultures
Elaut, G., Henkens, T., Papeleu, P., Snykers, S., Vinken, M., Vanhaecke, T., & Rogiers, V. (2006) · Current Drug Metabolism · doi:10.2174/138920006778017759 · Cited by 292 · see citing works
3D spheroid culture of hESC/hiPSC-derived hepatocyte-like cells for drug toxicity testing
Takayama, K., Kawabata, K., Nagamoto, Y., Kishimoto, K., Tashiro, K., Sakurai, F., Tachibana, M., Kanda, K., Hayakawa, T., Furue, M. K., et al. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.11.029 · Cited by 289 · see citing works
Native Umbilical Cord Matrix Stem Cells Express Hepatic Markers and Differentiate Into Hepatocyte-like Cells
Campard, D., Lysy, P. A., Najimi, M., & Sokal, E. M. (2007) · Gastroenterology · doi:10.1053/j.gastro.2007.12.024 · Cited by 287 · see citing works
A long-term three dimensional liver co-culture system for improved prediction of clinically relevant drug-induced hepatotoxicity
Kostadinova, R., Boess, F., Applegate, D., Suter, L., Weiser, T., Singer, T., Naughton, B., & Roth, A. (2013) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2013.01.012 · Cited by 262 · see citing works
The HepaRG cell line: a unique in vitro tool for understanding drug metabolism and toxicology in human
Andersson, T. B., Kanebratt, K. P., & Kenna, J. G. (2012) · Expert Opinion on Drug Metabolism & Toxicology · doi:10.1517/17425255.2012.685159 · Cited by 260 · see citing works
A microfluidically perfused three dimensional human liver model
Rennert, K., Steinborn, S., Gröger, M., Ungerböck, B., Jank, A., Ehgartner, J., Nietzsche, S., Dinger, J., Kiehntopf, M., Funke, H., et al. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.08.043 · Cited by 258 · see citing works
Modeling host interactions with hepatitis B virus using primary and induced pluripotent stem cell-derived hepatocellular systems
Shlomai, A., Schwartz, R. E., Ramanan, V., Bhatta, A., de Jong, Y. P., Bhatia, S. N., & Rice, C. M. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1412631111 · Cited by 258 · see citing works
Genetic variability overrides the impact of parental cell type and determines iPSC differentiation potential
Kyttälä, A., Moraghebi, R., Valensisi, C., Kettunen, J., Andrus, C., Pasumarthy, K. K., Nakanishi, M., Nishimura, K., Ohtaka, M., Weltner, J., et al. (2016) · Stem Cell Reports · doi:10.1016/j.stemcr.2015.12.009 · Cited by 257 · see citing works
Hepatocyte polarity
Treyer, A., & Müsch, A. (2013) · Comprehensive Physiology · doi:10.1002/cphy.c120009 · Cited by 252 · see citing works
Mechanisms of acetaminophen-induced cell death in primary human hepatocytes
Xie, Y., McGill, M. R., Dorko, K., Kumer, S. C., Schmitt, T. M., Forster, J., & Jaeschke, H. (2014) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2014.05.010 · Cited by 250 · see citing works
Efficient Generation of Functional Hepatocytes From Human Embryonic Stem Cells and Induced Pluripotent Stem Cells by HNF4α Transduction
Takayama, K., Inamura, M., Kawabata, K., Katayama, K., Higuchi, M., Tashiro, K., Nonaka, A., Sakurai, F., Hayakawa, T., Furue, M. K., et al. (2011) · Molecular Therapy · doi:10.1038/mt.2011.234 · Cited by 237 · see citing works
Culture matrix configuration and composition in the maintenance of hepatocyte polarity and function
Moghe, P. V., Francois Berthiaume, B., Ezzell, R. M., Toner, M., Tompkins, R. G., & Yarmush, M. L. (1996) · Biomaterials · doi:10.1016/0142-9612(96)85576-1 · Cited by 227 · see citing works
HepaRG human hepatic cell line utility as a surrogate for primary human hepatocytes in drug metabolism assessment in vitro
Lübberstedt, M., Müller‐Vieira, U., Mayer, M., Biemel, K. M., Knöspel, F., Knobeloch, D., Nussler, A. K., Gerlach, J. C., & Zeilinger, K. (2010) · Journal of Pharmacological and Toxicological Methods · doi:10.1016/j.vascn.2010.04.013 · Cited by 226 · see citing works
Self-citation
Reconstruction of Hepatic Organoid by Rat Small Hepatocytes and Hepatic Nonparenchymal Cells
Mitaka, T., Sato, F., Mizuguchi, T., Yokono, T., & Mochizuki, Y. (1999) · Hepatology · doi:10.1002/hep.510290103 · Cited by 221 · see citing works
Generation of functional cholangiocyte-like cells from human pluripotent stem cells and HepaRG cells
Dianat, N., Dubois‐Pot‐Schneider, H., Steichen, C., Desterke, C., Leclerc, P., Raveux, A., Combettes, L., Weber, A., Corlu, A., & Dubart‐Kupperschmitt, A. (2014) · Hepatology · doi:10.1002/hep.27165 · Cited by 213 · see citing works
Modeling hepatitis C virus infection using human induced pluripotent stem cells
Schwartz, R. E., Trehan, K., Andrus, L., Sheahan, T. P., Ploß, A., Duncan, S. A., Rice, C. M., & Bhatia, S. N. (2012) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1121400109 · Cited by 208 · see citing works
Use of Micropatterned Cocultures to Detect Compounds That Cause Drug-Induced Liver Injury in Humans
Khetani, S. R., Kanchagar, C., Ukairo, O. T., Krzyzewski, S., Moore, A., Shi, J., Aoyama, S., Aleo, M. D., & Will, Y. (2012) · Toxicological Sciences · doi:10.1093/toxsci/kfs326 · Cited by 208 · see citing works
Small-Molecule-Driven Hepatocyte Differentiation of Human Pluripotent Stem Cells
Siller, R., Greenhough, S., Naumovska, E., & Sullivan, G. J. (2015) · Stem Cell Reports · doi:10.1016/j.stemcr.2015.04.001 · Cited by 203 · see citing works
Maturation of Induced Pluripotent Stem Cell Derived Hepatocytes by 3D-Culture
Gieseck, R. L., Hannan, N. R. F., Bort, R., Hanley, N. A., Drake, R. A. L., Cameron, G. W. W., Wynn, T. A., & Vallier, L. (2014) · PLoS ONE · doi:10.1371/journal.pone.0086372 · Cited by 198 · see citing works
Prediction of Drug-Induced Liver Injury in Micropatterned Co-cultures Containing iPSC-Derived Human Hepatocytes
Ware, B. R., Berger, D. R., & Khetani, S. R. (2015) · Toxicological Sciences · doi:10.1093/toxsci/kfv048 · Cited by 196 · see citing works
Enhancing the functional maturity of induced pluripotent stem cell–derived human hepatocytes by controlled presentation of cell–cell interactions in vitro
Berger, D. R., Ware, B. R., Davidson, M. D., Allsup, S. R., & Khetani, S. R. (2014) · Hepatology · doi:10.1002/hep.27621 · Cited by 194 · see citing works
Prediction of interindividual differences in hepatic functions and drug sensitivity by using human iPS-derived hepatocytes
Takayama, K., Morisaki, Y., Kuno, S., Nagamoto, Y., Harada, K., Furukawa, N., Ohtaka, M., Nishimura, K., Imagawa, K., Sakurai, F., et al. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1413481111 · Cited by 193 · see citing works
Regulation of cell morphology and cytochrome P450 expression in human hepatocytes by extracellular matrix and cell-cell interactions
Coon, J., Hamilton, G. A., Jolley, S. L., Gilbert, D., Barros, S. A., & LeCluyse, E. L. (2001) · Cell and Tissue Research · doi:10.1007/s004410100429 · Cited by 192 · see citing works
Ammonia inhibition of protein degradation in isolated rat hepatocytes
Seglen, P. O., & Reith, A. (1976) · Experimental Cell Research · doi:10.1016/0014-4827(76)90148-8 · Cited by 175 · see citing works
Hepatic differentiation induced by oncostatin M attenuates fetal liver hematopoiesis
Kinoshita, T., Sekiguchi, T., Xu, M., Ito, Y., Kamiya, A., Tsuji, K.-I., Nakahata, T., & Miyajima, A. (1999) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.96.13.7265 · Cited by 172 · see citing works
Gene networks and transcription factor motifs defining the differentiation of stem cells into hepatocyte-like cells
Godoy, P., Schmidt‐Heck, W., Natarajan, K., Lucendo‐Villarin, B., Szkolnicka, D., Asplund, A., Björquist, P., Widera, A., Stöber, R. M., Campos, G., et al. (2015) · Journal of Hepatology · doi:10.1016/j.jhep.2015.05.013 · Cited by 170 · see citing works
Self-citation
Recombinant Laminins Drive the Differentiation and Self-Organization of hESC-Derived Hepatocytes
Cameron, K., Tan, R., Schmidt‐Heck, W., Campos, G., Lyall, M. J., Wang, Y., Lucendo‐Villarin, B., Szkolnicka, D., Bates, N., Kimber, S. J., et al. (2015) · Stem Cell Reports · doi:10.1016/j.stemcr.2015.10.016 · Cited by 149 · see citing works
Micropatterned coculture of primary human hepatocytes and supportive cells for the study of hepatotropic pathogens
March, S., Ramanan, V., Trehan, K., Ng, S., Galstian, A., Gural, N., Scull, M. A., Shlomai, A., Mota, M. M., Fleming, H. E., et al. (2015) · Nature Protocols · doi:10.1038/nprot.2015.128 · Cited by 147 · see citing works
Induced pluripotent stem cell-derived hepatocytes and endothelial cells in multi-component hydrogel fibers for liver tissue engineering
Du, C., Narayanan, K., Leong, M. F., & Wan, A. C. A. (2014) · Biomaterials · doi:10.1016/j.biomaterials.2014.04.011 · Cited by 144 · see citing works
Establishment of a Hepatocyte-Kupffer Cell Coculture Model for Assessment of Proinflammatory Cytokine Effects on Metabolizing Enzymes and Drug Transporters
Nguyen, T. V., Ukairo, O. T., Khetani, S. R., McVay, M., Kanchagar, C., Seghezzi, W., Ayanoglu, G., Irrechukwu, O., & Evers, R. (2015) · Drug Metabolism and Disposition · doi:10.1124/dmd.114.061317 · Cited by 137 · see citing works
Isolation of Primary Human Hepatocytes from Normal and Diseased Liver Tissue: A One Hundred Liver Experience
Bhogal, R. H., Hodson, J. A., Bartlett, D. C., Weston, C. J., Curbishley, S. M., Shepherd, E. L., Williams, K. T., Reynolds, G. M., Newsome, P. N., Adams, D., et al. (2011) · PLoS ONE · doi:10.1371/journal.pone.0018222 · Cited by 135 · see citing works
Stem cells in gastroenterology and hepatology
Quante, M., & Wang, T. C. (2009) · Nature Reviews Gastroenterology & Hepatology · doi:10.1038/nrgastro.2009.195 · Cited by 130 · see citing works
Immunohistochemical evidence for hepatic progenitor cells in liver diseases
Tan, J., Hytiroglou, P., Wieczorek, R. L., Park, Y. N., Thung, S. N., Arias, B., & Theise, N. D. (2002) · Liver International · doi:10.1034/j.1600-0676.2002.01622.x · Cited by 129 · see citing works
Hepatic 3D cultures but not 2D cultures preserve specific transporter activity for acetaminophen-induced hepatotoxicity
Schyschka, L., Sánchez, J. M., Wang, Z., Burkhardt, B., Müller‐Vieira, U., Zeilinger, K., Bachmann, A., Nadalin, S., Damm, G., & Nussler, A. K. (2013) · Archives of Toxicology · doi:10.1007/s00204-013-1080-y · Cited by 128 · see citing works
Self-citation
Sandwich-cultured hepatocytes: utility forin vitroexploration of hepatobiliary drug disposition and drug-induced hepatotoxicity
Bruyn, T. D., Chatterjee, S., Fattah, S., Keemink, J., Nicolaï, J., Augustijns, P., & Annaert, P. (2013) · Expert Opinion on Drug Metabolism & Toxicology · doi:10.1517/17425255.2013.773973 · Cited by 124 · see citing works
Hepatic Differentiation of Human Embryonic Stem Cells Is Promoted by Three-Dimensional Dynamic Perfusion Culture Conditions
Miki, T., Ring, A., & Gerlach, J. C. (2011) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2010.0437 · Cited by 121 · see citing works
Stellate cells: A moving target in hepatic fibrogenesis†
Friedman, S. L. (2004) · Hepatology · doi:10.1002/hep.20476 · Cited by 120 · see citing works
Scaling Down of a Clinical Three-Dimensional Perfusion Multicompartment Hollow Fiber Liver Bioreactor Developed for Extracorporeal Liver Support to an Analytical Scale Device Useful for Hepatic Pharmacological In Vitro Studies
Zeilinger, K., Schreiter, T., Darnell, M., Söderdahl, T., Lübberstedt, M., Dillnér, B., Knobeloch, D., Nussler, A. K., Gerlach, J. C., & Andersson, T. B. (2011) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2010.0580 · Cited by 118 · see citing works
Self-citation
Human iPSC-Derived Hepatocyte-like Cells Support Plasmodium Liver-Stage Infection In Vitro
Ng, S., Schwartz, R. E., March, S., Galstian, A., Gural, N., Shan, J., Prabhu, M., Mota, M. M., & Bhatia, S. N. (2015) · Stem Cell Reports · doi:10.1016/j.stemcr.2015.01.002 · Cited by 113 · see citing works
Use of primary human liver cells originating from discarded grafts in a bioreactor for liver support therapy and the prospects of culturing adult liver stem cells in bioreactors: a morphologic study
Gerlach, J. C., Mutig, K., Sauer, I. M., Schrade, P., Ефимова, Е. А., Mieder, T., Naumann, G., Grünwald, A., Pleß, G., Mas, A., et al. (2003) · Transplantation · doi:10.1097/01.tp.0000083319.36931.32 · Cited by 107 · see citing works
Self-citation
Accurate Prediction of Drug-Induced Liver Injury Using Stem Cell-Derived Populations
Szkolnicka, D., Farnworth, S. L., Lucendo‐Villarin, B., Storck, C., Zhou, W., Iredale, J. P., Flint, O. P., & Hay, D. C. (2013) · Stem Cells Translational Medicine · doi:10.5966/sctm.2013-0146 · Cited by 105 · see citing works
Dynamic interplay of flow and collagen stabilizes primary hepatocytes culture in a microfluidic platform
Hegde, M., Jindal, R., Bhushan, A., Bale, S. S., McCarty, W. J., Golberg, I., Usta, O. B., & Yarmush, M. L. (2014) · Lab on a Chip · doi:10.1039/c4lc00071d · Cited by 105 · see citing works
The promotion of hepatic maturation of human pluripotent stem cells in 3D co-culture using type I collagen and Swiss 3T3 cell sheets
Nagamoto, Y., Tashiro, K., Takayama, K., Ohashi, K., Kawabata, K., Sakurai, F., Tachibana, M., Hayakawa, T., Furue, M. K., & Mizuguchi, H. (2012) · Biomaterials · doi:10.1016/j.biomaterials.2012.03.011 · Cited by 104 · see citing works
Featured Article: Isolation, characterization, and cultivation of human hepatocytes and non-parenchymal liver cells
Pfeiffer, E., Kegel, V., Zeilinger, K., Hengstler, J. G., Nussler, A. K., Seehofer, D., & Damm, G. (2014) · Experimental Biology and Medicine · doi:10.1177/1535370214558025 · Cited by 104 · see citing works
Self-citation
Development of HepG2-derived cells expressing cytochrome P450s for assessing metabolism-associated drug-induced liver toxicity
Xuan, J., Chen, S., Ning, B., Tolleson, W. H., & Guo, L. (2016) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2015.10.009 · Cited by 103 · see citing works
Involvement of Cell Junctions in Hepatocyte Culture Functionality
Vinken, M., Papeleu, P., Snykers, S., Rop, E. D., Henkens, T., Chipman, J. K., Rogiers, V., & Vanhaecke, T. (2006) · Critical Reviews in Toxicology · doi:10.1080/10408440600599273 · Cited by 101 · see citing works
A comprehensive evaluation of metabolic activity and intrinsic clearance in suspensions and monolayer cultures of cryopreserved primary human hepatocytes
Smith, C. M., Nolan, C. K., Edwards, M., Hatfield, J. B., Stewart, T. W., Ferguson, S. S., LeCluyse, E. L., & Sahi, J. (2012) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.23262 · Cited by 99 · see citing works
Long-Term Chronic Toxicity Testing Using Human Pluripotent Stem Cell–Derived Hepatocytes
Holmgren, G., Sjogren, A.-K. M., Barragán, I., Sabirsh, A., Sartipy, P., Synnergren, J., Björquist, P., Ingelman‐Sundberg, M., Andersson, T. B., & Edsbagge, J. (2014) · Drug Metabolism and Disposition · doi:10.1124/dmd.114.059154 · Cited by 99 · see citing works
All-In-One: Advanced preparation of Human Parenchymal and Non-Parenchymal Liver Cells
Werner, M., Driftmann, S., Kleinehr, K., Kaiser, G. M., Mathé, Z., Treckmann, J. W., Paul, A., Skibbe, K., Timm, J., Canbay, A. E., et al. (2015) · PLoS ONE · doi:10.1371/journal.pone.0138655 · Cited by 91 · see citing works
“Epigenetic Memory” Phenomenon in Induced Pluripotent Stem Cells
Vaskova, E. A., Stekleneva, A. E., Medvedev, S. P., & Zakian, S. M. (2013) · Acta Naturae · doi:10.32607/20758251-2013-5-4-15-21 · Cited by 91 · see citing works
Directed differentiation of murine-induced pluripotent stem cells to functional hepatocyte-like cells
Sancho‐Bru, P., Roelandt, P. R., Narain, N., Pauwelyn, K. A., Notelaers, T., Shimizu, T., Ott, M., & Verfaillie, C. M. (2010) · Journal of Hepatology · doi:10.1016/j.jhep.2010.06.014 · Cited by 89 · see citing works
Differences in the Epigenetic Regulation of Cytochrome P450 Genes between Human Embryonic Stem Cell-Derived Hepatocytes and Primary Hepatocytes
Park, H., Choi, Y.-J., Kim, J. W., Chun, H., Im, I., Yoon, S., Han, Y., Song, C.-W., & Kim, H. (2015) · PLoS ONE · doi:10.1371/journal.pone.0132992 · Cited by 81 · see citing works
The protective effects of carvacrol and thymol against paracetamol–induced toxicity on human hepatocellular carcinoma cell lines (HepG2)
Palabıyık, Ş. S., Karakuş, E., Halıcı, Z., Cadirci, E., Bayır, Y., Ayaz, G. N., & Cinar, I. (2016) · Human & Experimental Toxicology · doi:10.1177/0960327115627688 · Cited by 79 · see citing works
Stiffness of hyaluronic acid gels containing liver extracellular matrix supports human hepatocyte function and alters cell morphology
Deegan, D. B., Zimmerman, C., Skardal, A., Atala, A., & Shupe, T. D. (2016) · Journal of the Mechanical Behavior of Biomedical Materials · doi:10.1016/j.jmbbm.2015.10.016 · Cited by 79 · see citing works
Gene Expression Profiling of Extracellular Matrix as an Effector of Human Hepatocyte Phenotype in Primary Cell Culture
Page, J. L., Johnson, M. C., Olsavsky, K. M., Strom, S. C., Zarbl, H., & Omiecinski, C. J. (2007) · Toxicological Sciences · doi:10.1093/toxsci/kfm034 · Cited by 77 · see citing works
Critical differences in toxicity mechanisms in induced pluripotent stem cell-derived hepatocytes, hepatic cell lines and primary hepatocytes
Sjogren, A.-K. M., Liljevald, M., Glinghammar, B., Sagemark, J., Li, X., Jonebring, A., Cotgreave, I. A., Brolén, G., & Andersson, T. B. (2014) · Archives of Toxicology · doi:10.1007/s00204-014-1265-z · Cited by 77 · see citing works
Development and characterization of human-induced pluripotent stem cell-derived cholangiocytes
de Assuncao, T. M., Sun, Y., Jalan‐Sakrikar, N., Drinane, M. C., Huang, B. Q., Li, Y., Davila, J. I., Wang, R., O’Hara, S. P., Lomberk, G. A., et al. (2015) · Laboratory Investigation · doi:10.1038/labinvest.2015.51 · Cited by 77 · see citing works
Recent advances in the morphological and functional heterogeneity of the biliary epithelium
Han, Y., Glaser, S. S., Meng, F., Francis, H., Marzioni, M., McDaniel, K. M., Alvaro, D., Venter, J. K., Carpino, G., Onori, P., et al. (2013) · Experimental Biology and Medicine · doi:10.1177/1535370213489926 · Cited by 75 · see citing works
Cytochrome P450-Dependent Metabolism in HepaRG Cells Cultured in a Dynamic Three-Dimensional Bioreactor
Darnell, M., Schreiter, T., Zeilinger, K., Urbaniak, T., Söderdahl, T., Rossberg, I., Dillnér, B., Berg, A., Gerlach, J. C., & Andersson, T. B. (2011) · Drug Metabolism and Disposition · doi:10.1124/dmd.110.037721 · Cited by 75 · see citing works
Self-citation
Cost-effective differentiation of hepatocyte-like cells from human pluripotent stem cells using small molecules
Tasnim, F., Phan, D., Toh, Y., & Yu, H. (2015) · Biomaterials · doi:10.1016/j.biomaterials.2015.08.002 · Cited by 73 · see citing works
Evaluation of Human Hepatocytes Cultured by Three-dimensional Spheroid Systems for Drug Metabolism
Ohkura, T., Ohta, K., Nagao, T., Kusumoto, K., Koeda, A., Ueda, T., Jomura, T., Ikeya, T., Ozeki, E., Wada, K., et al. (2014) · Drug Metabolism and Pharmacokinetics · doi:10.2133/dmpk.dmpk-13-rg-105 · Cited by 71 · see citing works
Analysis of drug metabolism activities in a miniaturized liver cell bioreactor for use in pharmacological studies
Hoffmann, S. A., Müller‐Vieira, U., Biemel, K. M., Knobeloch, D., Heydel, S., Lübberstedt, M., Nussler, A. K., Andersson, T. B., Gerlach, J. C., & Zeilinger, K. (2012) · Biotechnology and Bioengineering · doi:10.1002/bit.24573 · Cited by 69 · see citing works
Self-citation
Human hepatocytes and endothelial cells in organotypic membrane systems
Salerno, S., Campana, C., Morelli, S., Drioli, E., & Bartolo, L. D. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.08.004 · Cited by 68 · see citing works
DMSO Efficiently Down Regulates Pluripotency Genes in Human Embryonic Stem Cells during Definitive Endoderm Derivation and Increases the Proficiency of Hepatic Differentiation
Czysz, K., Minger, S. L., & Thomas, N. (2015) · PLoS ONE · doi:10.1371/journal.pone.0117689 · Cited by 68 · see citing works
Properties of Hepatocyte-like Cell Clusters from Human Adipose Tissue-Derived Mesenchymal Stem Cells
Okura, H., Komoda, H., Saga, A., Kakuta-Yamamoto, A., Hamada, Y., Fumimoto, Y., Lee, C. M., Ichinose, A., Sawa, Y., & Matsuyama, A. (2009) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2009.0208 · Cited by 67 · see citing works
HepaRG microencapsulated spheroids in DMSO-free culture: novel culturing approaches for enhanced xenobiotic and biosynthetic metabolism
Rebelo, S. P., Costa, R., Estrada, M. F., Shevchenko, V., Brito, C., & Alves, P. M. (2014) · Archives of Toxicology · doi:10.1007/s00204-014-1320-9 · Cited by 66 · see citing works
Application of Three-Dimensional Culture Conditions to Human Embryonic Stem Cell-Derived Definitive Endoderm Cells Enhances Hepatocyte Differentiation and Functionality
Ramasamy, T. S., Yu, J., Selden, C., Hodgson, H., & Cui, W. (2012) · Tissue Engineering Part A · doi:10.1089/ten.tea.2012.0190 · Cited by 66 · see citing works
Bioprinting Cellularized Constructs Using a Tissue-specific Hydrogel Bioink
Skardal, A., Devarasetty, M., Kang, H.-W., Seol, Y.-J., Forsythe, S. D., Bishop, C., Shupe, T., Soker, S., & Atala, A. (2016) · Journal of Visualized Experiments · doi:10.3791/53606 · Cited by 65 · see citing works
Morphological behaviour and metabolic capacity of cryopreserved human primary hepatocytes cultivated in a perfused multiwell device
Vivarès, A., Salle-Lefort, S., Arabeyre‐Fabre, C., Ngo, R., Pénarier, G., Bremond, M., Moliner, P., Gallas, J., Fabre, G., & Klieber, S. (2014) · Xenobiotica · doi:10.3109/00498254.2014.944612 · Cited by 64 · see citing works
Human Hepatocytes: Isolation, Culture, and Quality Procedures
Knobeloch, D., Ehnert, S., Schyschka, L., Büchler, P., Schoenberg, M., Kleeff, J. H., Thasler, W. E., Nüssler, N. C., Godoy, P., Hengstler, J. G., et al. (2011) · Methods in molecular biology · doi:10.1007/978-1-61779-367-7_8 · Cited by 63 · see citing works
Explanted Diseased Livers – A Possible Source of Metabolic Competent Primary Human Hepatocytes
Kleine, M., Riemer, M., Krech, T., DeTemple, D. E., Jäger, M. D., Lehner, F., Manns, M. P., Klempnauer, J. L., Borlak, J. T., Bektas, H., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0101386 · Cited by 63 · see citing works
Time Course of Primary Liver Cell Reorganization in Three-Dimensional High-Density Bioreactors for Extracorporeal Liver Support: An Immunohistochemical and Ultrastructural Study
Zeilinger, K., Holland, G., Sauer, I. M., Ефимова, Е. А., Kardassis, D., Obermayer, N., Liu, M., Neuhaus, P., & Gerlach, J. C. (2004) · Tissue Engineering · doi:10.1089/ten.2004.10.1113 · Cited by 62 · see citing works
Self-citation
Hepatic Differentiation and Maturation of Human Embryonic Stem Cells Cultured in a Perfused Three-Dimensional Bioreactor
Sivertsson, L., Synnergren, J., Jensen, J., Björquist, P., & Ingelman‐Sundberg, M. (2012) · Stem Cells and Development · doi:10.1089/scd.2012.0202 · Cited by 61 · see citing works
Enhanced In Vitro Maturation of Fetal Mouse Liver Cells with Oncostatin M, Nicotinamide, and Dimethyl Sulfoxide
Sakai, Y., Jiang, J., Kojima, N., Kinoshita, T., & Miyajima, A. (2002) · Cell Transplantation · doi:10.3727/000000002783985710 · Cited by 60 · see citing works
Human Pluripotent Stem Cells for Modelling Human Liver Diseases and Cell Therapy
Dianat, N., Steichen, C., Vallier, L., Weber, A., & Dubart‐Kupperschmitt, A. (2013) · Current Gene Therapy · doi:10.2174/1566523211313020006 · Cited by 60 · see citing works
Contribution of hepatic lineage stage-specific donor memory to the differential potential of induced mouse pluripotent stem cells (iPSC)
Lee, S. B., Seo, D., Choi, D., Park, K.-Y., Holczbauer, A., Marquardt, J. U., Conner, E., Factor, V., & Thorgeirsson, S. (2012) · Stem Cells · doi:10.1002/stem.1074 · Cited by 57 · see citing works
Hepatic Differentiation of Mesenchymal Stem Cells: In Vitro Strategies
Snykers, S., Kock, J. D., Vanhaecke, T., & Rogiers, V. (2011) · Methods in molecular biology · doi:10.1007/978-1-60761-999-4_23 · Cited by 56 · see citing works
Tissue collection, transport and isolation procedures required to optimize human hepatocyte isolation from waste liver surgical resections. A multilaboratory study
Richert, L., Alexandre, E., Lloyd, T. D. R., Orr, S., Viollon‐Abadie, C., Patel, R., Kingston, S., Berry, D. C., Dennison, A. R., Heyd, B., et al. (2004) · Liver International · doi:10.1111/j.1478-3231.2004.0930.x · Cited by 56 · see citing works
A Micropatterned Hepatocyte Coculture Model for Assessment of Liver Toxicity Using High-Content Imaging Analysis
Trask, O. J., Moore, A., & LeCluyse, E. L. (2014) · Assay and Drug Development Technologies · doi:10.1089/adt.2013.525 · Cited by 53 · see citing works
Effect of human patient plasma ex vivo treatment on gene expression and progenitor cell activation of primary human liver cells in multi‐compartment 3D perfusion bioreactors for extra‐corporeal liver support
Schmelzer, E., Mutig, K., Schrade, P., Bachmann, S., Gerlach, J. C., & Zeilinger, K. (2009) · Biotechnology and Bioengineering · doi:10.1002/bit.22283 · Cited by 53 · see citing works
Self-citation
Development of complex-shaped liver multicellular spheroids as a human-based model for nanoparticle toxicity assessment in vitro
Dubiak-Szepietowska, M., Karczmarczyk, A., Jönsson‐Niedziółka, M., Winckler, T., & Feller, K. ‐. (2016) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2016.01.016 · Cited by 52 · see citing works
High Throughput Micro-Well Generation of Hepatocyte Micro-Aggregates for Tissue Engineering
Gevaert, E., Dollé, L., Billiet, T., Dubruel, P., van Grunsven, L. A., van Apeldoorn, A. A., & Cornelissen, R. (2014) · PLoS ONE · doi:10.1371/journal.pone.0105171 · Cited by 51 · see citing works
The importance of the interaction between hepatocyte and hepatic stellate cells in fibrogenesis induced by fatty accumulation
Giraudi, P. J., Barbero-Becerra, V. J., Marin, V., Chávez-Tapia, N. C., Tiribelli, C., & Rosso, N. (2014) · Experimental and Molecular Pathology · doi:10.1016/j.yexmp.2014.12.006 · Cited by 51 · see citing works
Subtoxic Concentrations of Hepatotoxic Drugs Lead to Kupffer Cell Activation in a HumanIn VitroLiver Model: An Approach to Study DILI
Kegel, V., Pfeiffer, E., Burkhardt, B., Liu, J. L., Zeilinger, K., Nussler, A. K., Seehofer, D., & Damm, G. (2015) · Mediators of Inflammation · doi:10.1155/2015/640631 · Cited by 50 · see citing works
Self-citation
Toxicogenomics-based prediction of acetaminophen-induced liver injury using human hepatic cell systems
Rodrigues, R. M., Heymans, A., Boe, V. D., Sachinidis, A., Chaudhari, U., Govaere, O., Roskams, T., Vanhaecke, T., Rogiers, V., & Kock, J. D. (2015) · Toxicology Letters · doi:10.1016/j.toxlet.2015.10.014 · Cited by 50 · see citing works
Serum-free culture of primary human hepatocytes in a miniaturized hollow-fibre membrane bioreactor for pharmacologicalin vitrostudies
Lübberstedt, M., Müller‐Vieira, U., Biemel, K. M., Darnell, M., Hoffmann, S. A., Knöspel, F., Wönne, E. C., Knobeloch, D., Nussler, A. K., Gerlach, J. C., et al. (2012) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.1652 · Cited by 49 · see citing works
Self-citation
Dual-Purpose Bioreactors to Monitor Noninvasive Physical and Biochemical Markers of Kidney and Liver Scaffold Recellularization
Uzarski, J. S., Bijonowski, B. M., Wang, B., Ward, H. H., Wandinger‐Ness, A., Miller, W. M., & Wertheim, J. A. (2015) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2014.0665 · Cited by 49 · see citing works
One Standardized Differentiation Procedure Robustly Generates Homogenous Hepatocyte Cultures Displaying Metabolic Diversity from a Large Panel of Human Pluripotent Stem Cells
Asplund, A., Pradip, A., Giezen, M. V., Aspegren, A., Choukair, H., Rehnström, M., Jacobsson, S., Ghosheh, N., Hajjam, D. E., Holmgren, S., et al. (2015) · Stem Cell Reviews and Reports · doi:10.1007/s12015-015-9621-9 · Cited by 49 · see citing works
The effect of recombinant E-cadherin substratum on the differentiation of endoderm-derived hepatocyte-like cells from embryonic stem cells
Haque, A., Hexig, B., Meng, Q., Hossain, S., Nagaoka, M., & Akaike, T. (2010) · Biomaterials · doi:10.1016/j.biomaterials.2010.11.045 · Cited by 48 · see citing works
Development of novel tools for thein vitroinvestigation of drug-induced liver injury
Jiang, J., Wolters, J. E. J., Breda, S. G. J. V., Kleinjans, J. C. S., & de Kok, T. M. C. M. (2015) · Expert Opinion on Drug Metabolism & Toxicology · doi:10.1517/17425255.2015.1065814 · Cited by 46 · see citing works
Cryopreservation of human vascular umbilical cord cells under good manufacturing practice conditions for future cell banks
Polchow, B., Kebbel, K., Schmiedeknecht, G., Reichardt, A., Henrich, W., Hetzer, R., & Lueders, C. (2012) · Journal of Translational Medicine · doi:10.1186/1479-5876-10-98 · Cited by 44 · see citing works
In-depth physiological characterization of primary human hepatocytes in a 3D hollow-fiber bioreactor
Mueller, D., Tascher, G., Müller‐Vieira, U., Knobeloch, D., Nussler, A. K., Zeilinger, K., Heinzle, E., & Noor, F. (2011) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.418 · Cited by 43 · see citing works
Self-citation
Suppression of hepatocyte CYP1A2 expression by Kupffer cells via AhR pathway: The central role of proinflammatory cytokines
Wu, R., Cui, X., Dong, W., Zhou, M., Simms, H. H., & Wang, P. (2006) · International Journal of Molecular Medicine · doi:10.3892/ijmm.18.2.339 · Cited by 38 · see citing works
A Comparative Study of Genome-Wide Transcriptional Profiles of Primary Hepatocytes in Collagen Sandwich and Monolayer Cultures
Kim, Y.-H., Lasher, C. D., Milford, L. M., Murali, T. M., & Rajagopalan, P. (2010) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2010.0012 · Cited by 37 · see citing works
Isolation and Characterization of Adult Human Liver Progenitors from Ischemic Liver Tissue Derived from Therapeutic Hepatectomies
Stachelscheid, H., Urbaniak, T., Ring, A., Spengler, B., Gerlach, J. C., & Zeilinger, K. (2008) · Tissue Engineering Part A · doi:10.1089/ten.tea.2008.0291 · Cited by 37 · see citing works
Self-citation
Investigation of omeprazole and phenacetin first‐pass metabolism in humans using a microscale bioreactor and pharmacokinetic models
Bricks, T., Hamon, J., Fleury, M., Jellali, R., Merlier, F., Herpe, Y. E., Seyer, A., Regimbeau, J., Bois, F., & Leclerc, E. (2015) · Biopharmaceutics & drug disposition · doi:10.1002/bdd.1940 · Cited by 36 · see citing works
Human parenchymal and non-parenchymal liver cell isolation, culture and characterization
Damm, G., Pfeiffer, E., Burkhardt, B., Vermehren, J., Nüssler, A. K., & Weiss, T. S. (2013) · Hepatology International · doi:10.1007/s12072-013-9475-7 · Cited by 36 · see citing works
Self-citation
Upregulation of MiR‐122 via Trichostatin A Treatments in Hepatocyte‐like Cells Derived from Mesenchymal Stem Cells
Alizadeh, E., Eslaminejad, M. B., Akbarzadeh, A., Sadeghi, Z., Abasi, M., Herizchi, R., & Zarghami, N. (2015) · Chemical Biology & Drug Design · doi:10.1111/cbdd.12664 · Cited by 35 · see citing works
Primary hepatocyte cultures as prominentin vitrotools to study hepatic drug transporters
Ramboer, E., Vanhaecke, T., Rogiers, V., & Vinken, M. (2013) · Drug Metabolism Reviews · doi:10.3109/03602532.2012.756010 · Cited by 32 · see citing works
Hepatic Differentiation from Human Embryonic Stem Cells Using Stromal Cells
Yu, Y., Kim, K., Lee, S., Choi, S. Y., Kim, Y. C., Byun, K. S., Cha, I., Park, K.-Y., Cho, C. H., & Choi, D.-H. (2011) · Journal of Surgical Research · doi:10.1016/j.jss.2011.06.032 · Cited by 32 · see citing works
Polarized location of SLC and ABC drug transporters in monolayer-cultured human hepatocytes
Vée, M. L., Jouan, E., Noel, G. L., Stieger, B., & Fardel, O. (2015) · Toxicology in Vitro · doi:10.1016/j.tiv.2015.03.019 · Cited by 31 · see citing works
An Algorithm that Predicts the Viability and the Yield of Human Hepatocytes Isolated from Remnant Liver Pieces Obtained from Liver Resections
Lee, S. M. L., Schelcher, C., Laubender, R. P., Fröse, N., Thasler, R., Schiergens, T. S., Mansmann, U., & Thasler, W. E. (2014) · PLoS ONE · doi:10.1371/journal.pone.0107567 · Cited by 30 · see citing works
An in vitro model for hepatocyte-like cell differentiation from Wharton's jelly derived-mesenchymal stem cells by cell-base aggregates.
Talaei‐Khozani, T., Borhani‐Haghighi, M., Ayatollahi, M., & Vojdani, Z. (2015) · Gastroenterology and hepatology from bed to bench. · Cited by 30 · see citing works
HHEX Promotes Hepatic-Lineage Specification through the Negative Regulation of Eomesodermin
Watanabe, H., Takayama, K., Inamura, M., Tachibana, M., Mimura, N., Katayama, K., Tashiro, K., Nagamoto, Y., Sakurai, F., Kawabata, K., et al. (2014) · PLoS ONE · doi:10.1371/journal.pone.0090791 · Cited by 28 · see citing works
Improved cryopreservation of human hepatocytes using a new xeno free cryoprotectant solution
Saliem, M. (2012) · World Journal of Hepatology · doi:10.4254/wjh.v4.i5.176 · Cited by 27 · see citing works
Hepatic Differentiation of Human Embryonic Stem Cells as Microscaled Multilayered Colonies Leading to Enhanced Homogeneity and Maturation
Yao, R., Wang, J., Li, X., Jung, D. J., Qi, H., Kee, K., & Du, Y. (2014) · Small · doi:10.1002/smll.201401040 · Cited by 27 · see citing works
Characterization of hepatic markers in human Wharton’s Jelly-derived mesenchymal stem cells
Buyl, K., Kock, J. D., Najar, M., Lagneaux, L., Branson, S., Rogiers, V., & Vanhaecke, T. (2013) · Toxicology in Vitro · doi:10.1016/j.tiv.2013.06.014 · Cited by 27 · see citing works
Hepatocyte Culture in Autologous Decellularized Spleen Matrix
Gao, R., Wu, W., Xiang, J., Lv, Y., Zheng, X., Chen, Q., Wang, H., Wang, B., Liu, Z., & Ma, F. (2015) · Organogenesis · doi:10.1080/15476278.2015.1011908 · Cited by 26 · see citing works
AR42J-B-13 cell: An expandable progenitor to generate an unlimited supply of functional hepatocytes
Wallace, K., Fairhall, E. A., Charlton, K. A., & Wright, M. C. (2010) · Toxicology · doi:10.1016/j.tox.2010.05.008 · Cited by 26 · see citing works
Design of a Vitronectin-Based Recombinant Protein as a Defined Substrate for Differentiation of Human Pluripotent Stem Cells into Hepatocyte-Like Cells
Nagaoka, M., Kobayashi, M., Chie, K., Mallanna, S. K., & Duncan, S. A. (2015) · PLoS ONE · doi:10.1371/journal.pone.0136350 · Cited by 23 · see citing works
Preclinical Evaluation of Liposomal C8 Ceramide as a Potent anti-Hepatocellular Carcinoma Agent
Lv, H., Zhang, Z., Wu, X., Wang, Y., Li, C., Gong, W., Gui, L., & Wang, X. (2016) · PLoS ONE · doi:10.1371/journal.pone.0145195 · Cited by 22 · see citing works
Morphogenesis of liver epithelial cells
Tanimizu, N., & Mitaka, T. (2016) · Hepatology Research · doi:10.1111/hepr.12654 · Cited by 20 · see citing works
Stem Cells: Hepatic Differentiation of Human Embryonic Stem Cells as Microscaled Multilayered Colonies Leading to Enhanced Homogeneity and Maturation (Small 21/2014)
Yao, R., Wang, J., Li, X., Jung, D. J., Qi, H., Kee, K., & Du, Y. (2014) · Small · doi:10.1002/smll.201470133 · Cited by 19 · see citing works
Oxidative stress triggers cytokinesis failure in hepatocytes upon isolation
Tormos, A. M., Taléns‐Visconti, R., Ana Bonora-Centelles, A., Pérez, S., & Sastre, J. (2015) · Free Radical Research · doi:10.3109/10715762.2015.1016019 · Cited by 17 · see citing works
Cryopreservation of hepatic stellate cells
Neyzen, S., de Leur, E. V., Borkham‐Kamphorst, E., Herrmann, J., Hollweg, G., Gressner, A. M., & Weiskirchen, R. (2005) · Journal of Hepatology · doi:10.1016/j.jhep.2005.07.008 · Cited by 16 · see citing works
Promotion of Mesenchymal-to-Epithelial Transition by Rac1 Inhibition with Small Molecules Accelerates Hepatic Differentiation of Mesenchymal Stromal Cells
Teng, N.-Y., Liu, Y., Wu, H.-H., Liu, Y.-A., Ho, J. H., & Lee, O. K. (2015) · Tissue Engineering Part A · doi:10.1089/ten.tea.2014.0320 · Cited by 15 · see citing works
Generation of hepatocyte-like cells from human induced pluripotent stem (iPS) cells by co-culturing embryoid body cells with liver non-parenchymal cell line TWNT-1.
Javed, M. S., Yaqoob, N., Iwamuro, M., Kobayashi, N., & Fujiwara, T. (2014) · PubMed · Cited by 14 · see citing works
Cryopreservation and Enumeration of Human Endothelial Progenitor and Endothelial Cells for Clinical Trials
Bogoslovsky, T. (2013) · Journal of Blood Disorders & Transfusion · doi:10.4172/2155-9864.1000158 · Cited by 14 · see citing works
Induction of MITF expression in human cholangiocarcinoma cells and hepatocellular carcinoma cells by cyclopamine, an inhibitor of the Hedgehog signaling
Samatiwat, P., Takeda, K., Satarug, S., Ohba, K., Kukongviriyapan, V., & Shibahara, S. (2016) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2016.01.010 · Cited by 12 · see citing works
The B-13 hepatocyte progenitor cell resists pluripotency induction and differentiation to non-hepatocyte cells
Fairhall, E. A., Charles, M. A., Wallace, K., Schwab, C. J., Harrison, C. J., Richter, M., Hoffmann, S. A., Charlton, K. A., Zeilinger, K., & Wright, M. C. (2013) · Toxicology Research · doi:10.1039/c3tx50030f · Cited by 12 · see citing works
Self-citation
Characterization of rat and human Kupffer cells after cryopreservation
Walbrun, P., Hellerbrand, C., Weiß, T. S., Netter, S., Neumaier, D., Gäbele, E., Wiest, R., Schöelmerich, J., & Froh, M. (2007) · Cryobiology · doi:10.1016/j.cryobiol.2006.12.005 · Cited by 12 · see citing works
Human primary cultured hepatic stellate cells can be cryopreserved
Nakamura, A., Ueno, T., Yagi, Y., Okuda, K., Ogata, T., Nakamura, T., Torimura, T., Iwamoto, H., Ramadoss, S., Sata, M., et al. (2010) · Medical Molecular Morphology · doi:10.1007/s00795-009-0484-5 · Cited by 12 · see citing works
A Simple Isolation and Cryopreservation Method for Adult Human Hepatocytes
Hang, H., Shi, X., Gu, G., Wu, Y., & Ding, Y. (2009) · The International Journal of Artificial Organs · doi:10.1177/039139880903201003 · Cited by 11 · see citing works
Incubation with Dimethyl Sulfoxide Prior to Cryopreservation Improves Functionality of Thawed Human Primary Hepatocytes
Solanas, E., Sostres, C., Serrablo, A., Garcia‐Gil, A., Aranguren, F. J., Jimenez, P., & Serrano, T. (2012) · Biopreservation and Biobanking · doi:10.1089/bio.2012.0015 · Cited by 11 · see citing works
Adult human exocrine pancreas differentiation to hepatocytes – potential source of a human hepatocyte progenitor for use in toxicology research
Fairhall, E. A., Wallace, K., White, S. A., Huang, G. C., Shaw, J., Wright, S. C., Charlton, K. A., Burt, A. D., & Wright, M. C. (2012) · Toxicology Research · doi:10.1039/c2tx20061a · Cited by 10 · see citing works
Human Hepatocyte Isolation: Does Portal Vein Embolization Affect the Outcome?
Kluge, M., Reutzel‐Selke, A., Napierala, H., Hillebrandt, K. H., Major, R. D., Struecker, B., Leder, A., Siefert, J., Tang, P., Lippert, S., et al. (2015) · Tissue Engineering Part C Methods · doi:10.1089/ten.tec.2015.0190 · Cited by 10 · see citing works
Self-citation
Evaluating the Effects of Dithiothreitol and Fructose on Cell Viability and Function of Cryopreserved Primary Rat Hepatocytes and HepG2 Cell Line
Aghdaei, M. H., Jamshidzadeh, A., Nematizadeh, M., Behzadiannia, M., Niknahad, H., Amirghofran, Z., Esfandiari, E., & Azarpira, N. (2013) · Hepatitis Monthly · doi:10.5812/hepatmon.7824 · Cited by 10 · see citing works
Long-term culture and cryopreservation does not affect the stability and functionality of human embryonic stem cell-derived hepatocyte-like cells
Mandal, A., Raju, S., & Viswanathan, C. (2015) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-015-9956-1 · Cited by 8 · see citing works
Stem Cells in Pharmaceutical Biotechnology
Zuba‐Surma, E. K., Józkowicz, A., & Dulak, J. (2011) · Current Pharmaceutical Biotechnology · doi:10.2174/138920111798377120 · Cited by 8 · see citing works
The newly established human liver cell line: a potential cell source for the bioartificial liver in the future
Liu, H., You, S., Rong, Y., Wu, Y., Zhu, B., Zhihong, W., Liu, W., Panyong, M., & Xin, S. (2013) · Human Cell · doi:10.1007/s13577-013-0068-5 · Cited by 7 · see citing works
Construction and characterization of hepatocyte nuclear factor HNF4alpha1 over-expressing cell line derived from human hepatoma HepG2 cells
Novotná, A., Dořičáková, A., Vrzal, R., Pávek, P., & Dvořák, Z. (2011) · European Journal of Pharmacology · doi:10.1016/j.ejphar.2011.07.049 · Cited by 5 · see citing works
Development and characterization of a new human hepatic cell line
Ramboer, E., Craene, B. D., Kock, J. D., Berx, G., Rogiers, V., Vanhaecke, T., & Vinken, M. (2015) · EXCLI journal · doi:10.17179/excli2015-424 · Cited by 4 · see citing works
Pancreatic progenitor-derived hepatocytes are viable and functional in a 3D high density bioreactor culture system
Richter, M., Fairhall, E. A., Hoffmann, S. A., Tröbs, S., Knöspel, F., Probert, P. M. E., Oakley, F., Stroux, A., Wright, M. C., & Zeilinger, K. (2015) · Toxicology Research · doi:10.1039/c5tx00187k · Cited by 3 · see citing works
Self-citation

Flagged first, then by citations

Research works printed but not linked: 4

[26]26. Jiang J, Wolters JE, van Breda SG, Kleinjans JC, de Kok TM. Development of novel tools for the in vitro investigation of druginduced liver injury. Expert Opin Drug Metab Toxicol 2015; 11 :1523-37
[82]82. Darnell M, Schreiter T, Zeilinger K, Urbaniak T, So ¨ derdahl T, Rossberg I, Dillne ´r B, Berg AL, Gerlach JC, Andersson TB. Cytochrome P450dependent metabolism in HepaRG cells cultured in a dynamic threedimensional bioreactor. Drug Metab Dispos 2011; 39 :1131-8
[111]111. Cai J, DeLaForest A, Fisher J, Urick A, Wagner T, Twaroski K, Cayo M, Nagaoka M, Duncan SA. Protocol for directed differentiation of human pluripotent stem cells toward a hepatocyte fate. StemBook [Internet]. Cambridge, MA: Harvard Stem Cell Institute, 2012
[131]131. Sancho-Bru P, Roelandt P, Narain N, Pauwelyn K, Notelaers T, Shimizu T, Ott M, Verfaillie C. Directed differentiation of murineinduced pluripotent stemcells to functional hepatocyte-like cells. J Hepatol 2011; 54 :98-107

Sorted by citations

Modeling Steatohepatitis in Humans with Pluripotent Stem Cell-Derived Organoids
Ouchi, R., Togo, S., Kimura, M., Shinozawa, T., Koido, M., Koike, H., Thompson, W., Karns, R. A., Mayhew, C. N., McGrath, P. S., et al. (2019) · Cell Metabolism · doi:10.1016/j.cmet.2019.05.007 · Cited by 537 · see citing works
Scaffold-based 3D cell culture models in cancer research
Abuwatfa, W. H., Pitt, W. G., & Husseini, G. A. (2024) · Journal of Biomedical Science · doi:10.1186/s12929-024-00994-y · Cited by 347 · see citing works
3D cell culture models: Drug pharmacokinetics, safety assessment, and regulatory consideration
Wang, H., Brown, P. C., Chow, E. C. Y., Ewart, L. C., Ferguson, S., Fitzpatrick, S., Freedman, B., Guo, G. L., Hedrich, W. D., Heyward, S. A., et al. (2021) · Clinical and Translational Science · doi:10.1111/cts.13066 · Cited by 219 · see citing works
Interferon and Hepatitis B: Current and Future Perspectives
Ye, J., & Chen, J. (2021) · Frontiers in Immunology · doi:10.3389/fimmu.2021.733364 · Cited by 172 · see citing works
Engineering 3D Hydrogels for Personalized In Vitro Human Tissue Models
Liaw, C., Ji, S., & Güvendiren, M. (2018) · Advanced Healthcare Materials · doi:10.1002/adhm.201701165 · Cited by 170 · see citing works
A critical perspective on 3D liver models for drug metabolism and toxicology studies
Serras, A. S., Rodrigues, J. S., Cipriano, M., Rodrigues, A. V., Oliveira, N. G., & Miranda, J. P. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.626805 · Cited by 138 · see citing works
Models of Drug Induced Liver Injury (DILI) – Current Issues and Future Perspectives
Kuna, L., Krajina, I., Kizivat, T., Bojanić, K., Mrso, M., Kralj, E., Smolić, R., Wu, G. Y., & Smolić, M. (2018) · Current Drug Metabolism · doi:10.2174/1389200219666180523095355 · Cited by 138 · see citing works
In Vitro Models for Non-Alcoholic Fatty Liver Disease: Emerging Platforms and Their Applications
Ramos, M. J., Bandiera, L., Menolascina, F., & Fallowfield, J. A. (2022) · iScience · doi:10.1016/j.isci.2021.103549 · Cited by 127 · see citing works
Hypoglycemic and hypolipidemic effects of blueberry anthocyanins by AMPK activation: In vitro and in vivo studies
Herrera‐Balandrano, D. D., Chai, Z., Hutabarat, R. P., Trust Beta, Feng, J., Ma, K., Li, D., & Huang, W. (2021) · Redox Biology · doi:10.1016/j.redox.2021.102100 · Cited by 127 · see citing works
In Vitro and In Vivo Models of Non-Alcoholic Fatty Liver Disease: A Critical Appraisal
Soret, P.-A., Magusto, J., Housset, C., & Gautheron, J. (2020) · Journal of Clinical Medicine · doi:10.3390/jcm10010036 · Cited by 121 · see citing works
Human in vitro models of nonalcoholic fatty liver disease
Müller, F. A., & Sturla, S. J. (2019) · Current Opinion in Toxicology · doi:10.1016/j.cotox.2019.03.001 · Cited by 118 · see citing works
Liver Microphysiological Systems for Predicting and Evaluating Drug Effects
Ribeiro, A. J. S., Yang, X., Patel, V. P., Madabushi, R., & Strauss, D. G. (2019) · Clinical Pharmacology & Therapeutics · doi:10.1002/cpt.1458 · Cited by 109 · see citing works
Liver organoid as a 3D in vitro model for drug validation and toxicity assessment
Brooks, A., Liang, X., Zhang, Y., Zhao, C., Roberts, M. S., Wang, H., Zhang, L., & Crawford, D. H. (2021) · Pharmacological Research · doi:10.1016/j.phrs.2021.105608 · Cited by 109 · see citing works
Liver organ-on-chip models for toxicity studies and risk assessment
Messelmani, T., Morisseau, L., Sakai, Y., Legallais, C., Goff, A. L., Leclerc, É., & Jellali, R. (2022) · Lab on a Chip · doi:10.1039/d2lc00307d · Cited by 110 · see citing works
Stem-cell based organ-on-a-chip models for diabetes research
Rogal, J., Zbinden, A., Schenke‐Layland, K., & Loskill, P. (2018) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2018.10.010 · Cited by 83 · see citing works
Mesenchymal Stem Cells for the Treatment of Liver Disease: Present and Perspectives
Kang, S. H., Kim, M. Y., Eom, Y. W., & Baik, S. K. (2020) · Gut and Liver · doi:10.5009/gnl18412 · Cited by 78 · see citing works
From vision toward best practices: Evaluating in vitro transcriptomic points of departure for application in risk assessment using a uniform workflow
Reardon, A. J. F., Farmahin, R., Williams, A., Meier, M. J., Addicks, G. C., Yauk, C. L., Matteo, G., Atlas, E., Harrill, J., Everett, L. J., et al. (2023) · Frontiers in Toxicology · doi:10.3389/ftox.2023.1194895 · Cited by 73 · see citing works
Liver cell therapy: is this the end of the beginning?
Alwahsh, S. M., Rashidi, H., & Hay, D. C. (2017) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-017-2713-8 · Cited by 70 · see citing works
Microfluidic Organ-on-a-Chip Devices for Liver Disease Modeling In Vitro
Kanabekova, P., Kadyrova, A., & Kulsharova, G. (2022) · Micromachines · doi:10.3390/mi13030428 · Cited by 72 · see citing works
Preclinical models of idiosyncratic drug-induced liver injury (iDILI): Moving towards prediction
Segovia‐Zafra, A., Zeo-Sánchez, D. E. D., Gomez, C. L., Pérez-Valdés, Z., García‐Fuentes, E., Andrade, R. J., Lucena, M. I., & Paz, M. V. (2021) · Acta Pharmaceutica Sinica B · doi:10.1016/j.apsb.2021.11.013 · Cited by 71 · see citing works
In vitro Models and On-Chip Systems: Biomaterial Interaction Studies With Tissues Generated Using Lung Epithelial and Liver Metabolic Cell Lines
Nikolic, M. M., Geroski, T., & Filipović, N. (2018) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2018.00120 · Cited by 62 · see citing works
Three-dimensional (3D) liver cell models - a tool for bridging the gap between animal studies and clinical trials when screening liver accumulation and toxicity of nanobiomaterials
Tutty, M. A., Movia, D., & Prina‐Mello, A. (2022) · Drug Delivery and Translational Research · doi:10.1007/s13346-022-01147-0 · Cited by 64 · see citing works
A nonalcoholic fatty liver disease model in human induced pluripotent stem cell-derived hepatocytes, created by endoplasmic reticulum stress-induced steatosis
Parafati, M., Kirby, R. J., Khorasanizadeh, S., Rastinejad, F., & Malany, S. (2018) · Disease Models & Mechanisms · doi:10.1242/dmm.033530 · Cited by 61 · see citing works
Mechanisms and in vitro models of drug-induced cholestasis
Gijbels, E., Vilas‐Boas, V., Deferm, N., Devisscher, L., Jaeschke, H., Annaert, P., & Vinken, M. (2019) · Archives of Toxicology · doi:10.1007/s00204-019-02437-2 · Cited by 56 · see citing works
Liver–heart on chip models for drug safety
Ferrari, E., & Rasponi, M. (2021) · APL Bioengineering · doi:10.1063/5.0048986 · Cited by 53 · see citing works
In Vivo and In Vitro Models of Hepatocellular Carcinoma: Current Strategies for Translational Modeling
Romualdo, G. R., Leroy, K., Costa, C. J. S., Bacil, G. P., Vanderborght, B., da Silva, T. C., Barbisan, L. F., Andraus, W., Devisscher, L., Câmara, N. O. S., et al. (2021) · Cancers · doi:10.3390/cancers13215583 · Cited by 52 · see citing works
In Vitro Three‐Dimensional Liver Models for Nanomaterial DNA Damage Assessment
Llewellyn, S. V., Niemeijer, M., Nymark, P., Moné, M. J., van de Water, B., Conway, G. E., Jenkins, G. J. S., & Doak, S. H. (2021) · Small · doi:10.1002/smll.202006055 · Cited by 41 · see citing works
Engineering Organ-on-a-Chip to Accelerate Translational Research
Ko, J., Park, D., Lee, S., Gümüşcü, B., & Jeon, N. L. (2022) · Micromachines · doi:10.3390/mi13081200 · Cited by 52 · see citing works
A Modern Genotoxicity Testing Paradigm: Integration of the High-Throughput CometChip® and the TGx-DDI Transcriptomic Biomarker in Human HepaRG™ Cell Cultures
Buick, J. K., Williams, A., Meier, M. J., Swartz, C. D., Recio, L., Gagné, R., Ferguson, S., Engelward, B. P., & Yauk, C. L. (2021) · Frontiers in Public Health · doi:10.3389/fpubh.2021.694834 · Cited by 47 · see citing works
Shedding light on non-alcoholic fatty liver disease: Pathogenesis, molecular mechanisms, models, and emerging therapeutics
Khairnar, R., Islam, M. A., Fleishman, J. S., & Kumar, S. (2022) · Life Sciences · doi:10.1016/j.lfs.2022.121185 · Cited by 46 · see citing works
Quantitative comparison of in vitro genotoxicity between metabolically competent HepaRG cells and HepG2 cells using the high-throughput high-content CometChip assay
Seo, J., Tryndyak, V., Wu, Q., Dreval, K., Pogribny, I. P., Bryant, M., Zhou, T., Robison, T. W., Mei, N., & Guo, X. (2019) · Archives of Toxicology · doi:10.1007/s00204-019-02406-9 · Cited by 46 · see citing works
Advanced Organ-on-a-Chip Devices to Investigate Liver Multi-Organ Communication: Focus on Gut, Microbiota and Brain
Boeri, L., Izzo, L., Sardelli, L., Tunesi, M., Albani, D., & Giordano, C. (2019) · Bioengineering · doi:10.3390/bioengineering6040091 · Cited by 45 · see citing works
Development of organoid-based drug metabolism model
Park, E., Kim, H. K., Jee, J., Hahn, S., Jeong, S., & Yoo, J. (2019) · Toxicology and Applied Pharmacology · doi:10.1016/j.taap.2019.114790 · Cited by 44 · see citing works
Critical Considerations for the Design of Multi-Organ Microphysiological Systems (MPS)
Malik, M., Yang, Y., Fathi, P., Mahler, G. J., & Esch, M. B. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.721338 · Cited by 43 · see citing works
From organoids to organs: Bioengineering liver grafts from hepatic stem cells and matrix
Willemse, J., Lieshout, R., van der Laan, L. J. W., & Verstegen, M. M. A. (2017) · Best Practice & Research Clinical Gastroenterology · doi:10.1016/j.bpg.2017.03.003 · Cited by 44 · see citing works
A Standardized Collagen-Based Scaffold Improves Human Hepatocyte Shipment and Allows Metabolic Studies over 10 Days
Ruoß, M., Häussling, V., Schügner, F., Damink, L. H. H. O., Lee, S. M. L., Ge, L., Ehnert, S., & Nussler, A. K. (2018) · Bioengineering · doi:10.3390/bioengineering5040086 · Cited by 43 · see citing works
Sex difference in liver diseases: How preclinical models help to dissect the sex-related mechanisms sustaining NAFLD and hepatocellular carcinoma
Smiriglia, A., Lorito, N., Serra, M., Perra, A., Morandi, A., & Kowalik, M. A. (2023) · iScience · doi:10.1016/j.isci.2023.108363 · Cited by 42 · see citing works
Use of HuH6 and other human-derived hepatoma lines for the detection of genotoxins: a new hope for laboratory animals?
Waldherr, M., Mišík, M., Ferk, F., Tomc, J., Žegura, B., Filipič, M., Mikulits, W., Mai, S., Haas, O. A., Huber, W. W., et al. (2017) · Archives of Toxicology · doi:10.1007/s00204-017-2109-4 · Cited by 42 · see citing works
In Vitro Models of the Liver: Disease Modeling, Drug Discovery and Clinical Applications
Collins, S. D., Yuen, G., Tu, T., Budzinska, M. A., Spring, K. J., Bryant, K., & Shackel, N. A. (2019) · Codon Publications eBooks · doi:10.15586/hepatocellularcarcinoma.2019.ch3 · Cited by 41 · see citing works
<i>In vitro</i> functional models for human liver diseases and drug screening: beyond animal testing
Paradiso, A., Volpi, M., Rinoldi, C., Celikkin, N., Contessi Negrini, N., Bilgen, M., Dallera, G., Pierini, F., Costantini, M., Święszkowski, W., et al. (2023) · Biomaterials Science · doi:10.1039/d1bm01872h · Cited by 40 · see citing works
3D bioprinting of dECM-incorporated hepatocyte spheroid for simultaneous promotion of cell-cell and -ECM interactions
Kim, M. K., Jeong, W., Jeon, S., & Kang, H.-W. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1305023 · Cited by 38 · see citing works
Extracellular vesicles in hepatology: Physiological role, involvement in pathogenesis, and therapeutic opportunities
Azparren‐Angulo, M., Royo, F., González, E., Patricio-Liébana, M., Brotons, B., Berganza, J., Goñi‐de‐Cerio, F., Manicardi, N., Abad‐Jordà, L., Gracia‐Sancho, J., et al. (2020) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2020.107683 · Cited by 38 · see citing works
Hepatocellular Carcinoma
Carlessi, R., Köhn‐Gaone, J., Olynyk, J. K., & Tirnitz‐Parker, J. E. E. (2019) · Codon Publications eBooks · doi:10.15586/hepatocellularcarcinoma.2019 · Cited by 37 · see citing works
The Material World of 3D‐Bioprinted and Microfluidic‐Chip Models of Human Liver Fibrosis
Carvalho, A. M., Bansal, R., Barrias, C. C., & Sarmento, B. F. C. C. (2023) · Advanced Materials · doi:10.1002/adma.202307673 · Cited by 36 · see citing works
Organs-on-a-chip
Bovard, D., Iskandar, A. R., Luettich, K., Hoeng, J., & Peitsch, M. C. (2017) · Toxicology Research and Application · doi:10.1177/2397847317726351 · Cited by 35 · see citing works
IL6 supports long-term expansion of hepatocytes in vitro
Guo, R., Jiang, M., Wang, G., Li, B., Jia, X., Ai, Y., Chen, S., Tang, P., Liu, A., Yuan, Q., et al. (2022) · Nature Communications · doi:10.1038/s41467-022-35167-8 · Cited by 36 · see citing works
Histone lysine methyltransferase structure activity relationships that allow for segregation of G9a inhibition and anti-Plasmodium activity
Sundriyal, S., Chen, P. B., Lubin, A., Lueg, G. A., Li, F., White, A. J. P., Malmquist, N. A., Vedadi, M., Scherf, A., & Fuchter, M. J. (2017) · MedChemComm · doi:10.1039/c7md00052a · Cited by 33 · see citing works
Assessment of Hepatotoxic Potential of Cyanobacterial Toxins Using 3D In Vitro Model of Adult Human Liver Stem Cells
Basu, A., Dydowiczová, A., Čtveráčková, L., Jaša, L., Trosko, J. E., Bláha, L., & Babica, P. (2018) · Environmental Science & Technology · doi:10.1021/acs.est.8b02291 · Cited by 34 · see citing works
Hepatoprotective activity of medicinal plants: A mini review
Jain, D., Chaudhary, P., Kotnala, A., Hossain, R., Bisht, K., & Hossain, M. N. (2020) · Journal of Medicinal Plants Studies · doi:10.22271/plants.2020.v8.i5c.1212 · Cited by 33 · see citing works
Modeling drug-induced liver injury: current status and future prospects
Zeo-Sánchez, D. E. D., Segovia‐Zafra, A., Matilla‐Cabello, G., Pinazo-Bandera, J. M., Andrade, R. J., Lucena, M. I., & Paz, M. V. (2022) · Expert Opinion on Drug Metabolism & Toxicology · doi:10.1080/17425255.2022.2122810 · Cited by 33 · see citing works
Recent advances in models of immune-mediated drug-induced liver injury
Tasnim, F., Huang, X., Lee, C. Z. W., Ginhoux, F., & Yu, H. (2021) · Frontiers in Toxicology · doi:10.3389/ftox.2021.605392 · Cited by 32 · see citing works
Therapeutic efficiency of bone marrow-derived mesenchymal stem cells for liver fibrosis: A systematic review of in vivo studies
Al‐Dhamin, Z., Liu, L., Li, D., Zhang, S., Dong, S., & Nan, Y. (2020) · World Journal of Gastroenterology · doi:10.3748/wjg.v26.i47.7444 · Cited by 30 · see citing works
Mechanisms of Chronic Fialuridine Hepatotoxicity as Revealed in Primary Human Hepatocyte Spheroids
Hendriks, D. F. G., Hurrell, T., Riede, J., van der Horst, M., Tuovinen, S., & Ingelman-Sundberg, M. (2019) · Toxicological Sciences · doi:10.1093/toxsci/kfz195 · Cited by 29 · see citing works
Mouse Models of Hepatocellular Carcinoma: Classification, Advancement, and Application
Liu, S., Huang, F., Ru, G., Wang, Y., Zhang, B., Chen, X., & Chu, L. (2022) · Frontiers in Oncology · doi:10.3389/fonc.2022.902820 · Cited by 29 · see citing works
The flower of Hypericum perforatum L.: A traditional source of bioactives for new food and pharmaceutical applications
Kapoor, S., Chandel, R., Kaur, R., Kumar, S., Kumar, R., Janghu, S., Kaur, A., & Kumar, V. (2023) · Biochemical Systematics and Ecology · doi:10.1016/j.bse.2023.104702 · Cited by 29 · see citing works
The application of omics-based human liver platforms for investigating the mechanism of drug-induced hepatotoxicity in vitro
Jiang, J., Pieterman, C., Ertaylan, G., Peeters, R., & de Kok, T. M. C. M. (2019) · Archives of Toxicology · doi:10.1007/s00204-019-02585-5 · Cited by 31 · see citing works
Hydrogel-based preparation of cell aggregates for biomedical applications
Zhang, J., Yun, S., Du, Y., Zannettino, A. C. W., & Zhang, H. (2020) · Applied Materials Today · doi:10.1016/j.apmt.2020.100747 · Cited by 29 · see citing works
Body-in-a-Cube: a microphysiological system for multi-tissue co-culture with near-physiological amounts of blood surrogate
Chen, L., Yang, Y., Ueno, H., & Esch, M. B. (2020) · Microphysiological Systems · doi:10.21037/mps-19-8 · Cited by 28 · see citing works
Induction and Maturation of Hepatocyte-Like Cells In Vitro: Focus on Technological Advances and Challenges
Xie, Y., Yao, J., Jin, W., Ren, L., & Li, X. (2021) · Frontiers in Cell and Developmental Biology · doi:10.3389/fcell.2021.765980 · Cited by 27 · see citing works
Advancements in the Alcohol-Associated Liver Disease Model
Zhu, L., Li, H.-D., Xu, J., Li, J., Cheng, M., Meng, X., Huang, C., & Li, J. (2022) · Biomolecules · doi:10.3390/biom12081035 · Cited by 30 · see citing works
The long-chain monounsaturated cetoleic acid improves the efficiency of the n-3 fatty acid metabolic pathway in Atlantic salmon and human HepG2 cells
Østbye, T.-K. K., Berge, G. M., Nilsson, A., Romarheim, O. H., Bou, M., & Ruyter, B. (2019) · British Journal Of Nutrition · doi:10.1017/s0007114519001478 · Cited by 27 · see citing works
Direct reprogramming of somatic cells into induced hepatocytes: Cracking the Enigma code
Rombaut, M., Boeckmans, J., Rodrigues, R. M., van Grunsven, L. A., Vanhaecke, T., & Kock, J. D. (2021) · Journal of Hepatology · doi:10.1016/j.jhep.2021.04.048 · Cited by 26 · see citing works
Generation of Transplantable Three-Dimensional Hepatic-Patch to Improve the Functionality of Hepatic Cells In Vitro and In Vivo
Lahrood, F. N., Saheli, M., Farzaneh, Z., Taheri, P., Dorraj, M., Baharvand, H., Vosough, M., & Piryaei, A. (2019) · Stem Cells and Development · doi:10.1089/scd.2019.0130 · Cited by 25 · see citing works
Chemogenomic Screening in a Patient‐Derived 3D Fatty Liver Disease Model Reveals the CHRM1‐TRPM8 Axis as a Novel Module for Targeted Intervention
Youhanna, S. C., Kemas, A. M., Wright, S. C., Zhong, Y., Klumpp, B., Klein, K., Motso, A., Michel, M., Ziegler, N., Shang, M.-M., et al. (2024) · Advanced Science · doi:10.1002/advs.202407572 · Cited by 25 · see citing works
Hollow Fiber and Nanofiber Membranes in Bioartificial Liver and Neuronal Tissue Engineering
Morelli, S., Piscioneri, A., Salerno, S., & Bartolo, L. D. (2021) · Cells Tissues Organs · doi:10.1159/000511680 · Cited by 25 · see citing works
Application of microfluidic chips in the simulation of the urinary system microenvironment
Hou, C., Gu, Y., Yuan, W., Zhang, W., Xiu, X., Lin, J., Gao, Y., Liu, P., Chen, X., & Song, L. (2023) · Materials Today Bio · doi:10.1016/j.mtbio.2023.100553 · Cited by 26 · see citing works
A Human Ovarian Tumor & Liver Organ-on-Chip for Simultaneous and More Predictive Toxo-Efficacy Assays
Fedi, A., Vitale, C., Fato, M., & Scaglione, S. (2023) · Bioengineering · doi:10.3390/bioengineering10020270 · Cited by 26 · see citing works
Microfluidic organ-on-a-chip models for the gut–liver axis: from structural mimicry to functional insights
Hu, W., Wang, Y., Han, J., Zhang, W., Chen, J., Li, X., & Wang, L. (2025) · Biomaterials Science · doi:10.1039/d4bm01273a · Cited by 26 · see citing works
The Performance of HepG2 and HepaRG Systems through the Glass of Acetaminophen-Induced Toxicity
Lőrincz, T., Deák, V., Makk‐Merczel, K., Varga, D., Hajdinák, P., & Szarka, A. (2021) · Life · doi:10.3390/life11080856 · Cited by 24 · see citing works
Modelling urea cycle disorders using iPSCs
Duff, C. E., & Baruteau, J. (2022) · npj Regenerative Medicine · doi:10.1038/s41536-022-00252-5 · Cited by 23 · see citing works
Modelling fatty liver disease with mouse liver-derived multicellular spheroids
van Os, E. A., Cools, L., Eysackers, N., Szafranska, K., Smout, A., Verhulst, S., Reynaert, H., McCourt, P., Mannaerts, I., & van Grunsven, L. A. (2022) · Biomaterials · doi:10.1016/j.biomaterials.2022.121817 · Cited by 25 · see citing works
Performance of high-throughput CometChip assay using primary human hepatocytes: a comparison of DNA damage responses with in vitro human hepatoma cell lines
Seo, J., Wu, Q., Bryant, M., Ren, L., Shi, Q., Robison, T. W., Mei, N., Manjanatha, M. G., & Guo, X. (2020) · Archives of Toxicology · doi:10.1007/s00204-020-02736-z · Cited by 23 · see citing works
Detection of acute toxicity of aflatoxin B1 to human hepatocytes in vitro and in vivo using chimeric mice with humanized livers
Ishida, Y., Yamasaki, C., Iwanari, H., Yamashita, H., Ogawa, Y., Yanagi, A., Furukawa, S., Kojima, Y., Chayama, K., Kamiie, J., et al. (2020) · PLoS ONE · doi:10.1371/journal.pone.0239540 · Cited by 23 · see citing works
Can Transcriptomic Profiles from Cancer Cell Lines Be Used for Toxicity Assessment?
Liu, Z., Zhu, L., Thakkar, S., Roberts, R. A., & Tong, W. (2019) · Chemical Research in Toxicology · doi:10.1021/acs.chemrestox.9b00288 · Cited by 21 · see citing works
Novel organoid model in drug screening: Past, present, and future
Nie, X., Liang, Z., Li, K., Yu, H., Huang, Y., Ye, L., & Yang, Y. (2021) · Liver Research · doi:10.1016/j.livres.2021.05.003 · Cited by 22 · see citing works
In vitro cell-based models of drug-induced hepatotoxicity screening: progress and limitation
Mirahmad, M., Sabourian, R., Mahdavi, M. R., Larijani, B. A., & Safavi, M. (2022) · Drug Metabolism Reviews · doi:10.1080/03602532.2022.2064487 · Cited by 20 · see citing works
Essential phospholipids decrease apoptosis and increase membrane transport in human hepatocyte cell lines
Wupperfeld, D., Fricker, G., Fer, B. B. D., Frank, L., Wehrle, A., & Popovic, B. (2022) · Lipids in Health and Disease · doi:10.1186/s12944-022-01698-8 · Cited by 21 · see citing works
Hepatitis B virus infection modeling using multi-cellular organoids derived from human induced pluripotent stem cells
Cao, D., Ge, J.-Y., Wang, Y., Oda, T., & Zheng, Y. (2021) · World Journal of Gastroenterology · doi:10.3748/wjg.v27.i29.4784 · Cited by 19 · see citing works
Hepatic organoids as a platform for liver disease modeling and the development of novel therapies
Luce, E., Messina, A., & Duclos‐Vallée, J. (2025) · Clinics and Research in Hepatology and Gastroenterology · doi:10.1016/j.clinre.2025.102647 · Cited by 20 · see citing works
Reproducibility and Robustness of a Liver Microphysiological System PhysioMimix LC12 under Varying Culture Conditions and Cell Type Combinations
Lim, A. Y., Kato, Y., Sakolish, C., Valdiviezo, A., Han, G., Bajaj, P., Stanko, J. P., Ferguson, S., Villenave, R., Hewitt, P. G., et al. (2023) · Bioengineering · doi:10.3390/bioengineering10101195 · Cited by 19 · see citing works
Microengineered systems with iPSC-derived cardiac and hepatic cells to evaluate drug adverse effects
Dame, K., & Ribeiro, A. J. S. (2020) · Experimental Biology and Medicine · doi:10.1177/1535370220959598 · Cited by 19 · see citing works
HepaRG Maturation in Silk Fibroin Scaffolds: Toward Developing a 3D In Vitro Liver Model
Abbott, A., & Coburn, J. M. (2021) · ACS Biomaterials Science & Engineering · doi:10.1021/acsbiomaterials.0c01584 · Cited by 19 · see citing works
High resolution stereolithography fabrication of perfusable scaffolds to enable long-term meso-scale hepatic culture for disease modeling
Sphabmixay, P., Raredon, M. S. B., Wang, A. J.-S., Lee, H., Hammond, P. T., Fang, N. X., & Griffith, L. G. (2021) · Biofabrication · doi:10.1088/1758-5090/ac23aa · Cited by 20 · see citing works
Deciphering the Diversified Metabolic Behavior of Hydroxyalkyl Ferrocidiphenols as Anticancer Complexes
Wang, H., Fan, X., Xie, P., Yang, S. C., Pigeon, P., Xiong, Y., Gai, S., Qi, X., Wang, J., Zhang, Q., et al. (2023) · Journal of Medicinal Chemistry · doi:10.1021/acs.jmedchem.3c01709 · Cited by 18 · see citing works
Human iPSC-derived liver co-culture spheroids to model liver fibrosis
Cools, L., Dastjerd, M. K., Smout, A., Merens, V., Yang, Y., Reynaert, H., Messaoudi, N., Smet, V. D., Kumar, M., Verhulst, S., et al. (2024) · Biofabrication · doi:10.1088/1758-5090/ad5766 · Cited by 18 · see citing works
Bioactivities of decoctions from Plectranthus species related to their traditional use on the treatment of digestive problems and alcohol intoxication
Brito, E., Gomes, E. T., Fale, P. L. V., Borges, C., Pacheco, R., Teixeira, V. H., Machuqueiro, M., Ascensão, L., & Serralheiro, M. L. (2018) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2018.04.006 · Cited by 18 · see citing works
Mapping Proteome and Lipidome Changes in Early-Onset Non-Alcoholic Fatty Liver Disease Using Hepatic 3D Spheroids
Frandsen, H. S., Vej-Nielsen, J. M., Smith, L. E., Sun, L., Mikkelsen, K. L., Thulesen, A. P., Hagensen, C. E., Yang, F., & Rogowska-Wrzesińska, A. (2022) · Cells · doi:10.3390/cells11203216 · Cited by 17 · see citing works
Modeling alcohol-associated liver disease in humans using adipose stromal or stem cell-derived organoids
Bi, G., Zhang, X., Li, W., Lu, X., He, X., Li, Y., Bai, R., & Zhang, H. (2024) · Cell Reports Methods · doi:10.1016/j.crmeth.2024.100778 · Cited by 17 · see citing works
Point-of-use, automated fabrication of a 3D human liver model supplemented with human adipose microvessels
Moss, S. M., Schilp, J., Yaakov, M., Cook, M., Schuschke, E., Hanke, B., Strobel, H. A., & Hoying, J. B. (2022) · SLAS DISCOVERY · doi:10.1016/j.slasd.2022.06.003 · Cited by 16 · see citing works
Human induced pluripotent stem cell–derived liver-on-a-chip for studying drug metabolism: the challenge of the cytochrome P450 family
Tamargo-Rubio, I., Simpson, A. B., Hoogerland, J. A., & Fu, J. (2023) · Frontiers in Pharmacology · doi:10.3389/fphar.2023.1223108 · Cited by 17 · see citing works
Human Pluripotent Stem-Cell-Derived Models as a Missing Link in Drug Discovery and Development
Lin, X., Tang, J., & Lou, Y. (2021) · Pharmaceuticals · doi:10.3390/ph14060525 · Cited by 16 · see citing works
Analyzing the metabolic fate of oral administration drugs: A review and state-of-the-art roadmap
Liu, L., Liu, Y., Zhou, X., Xu, Z., Zhang, Y., Ji, L., Hong, C., & Li, C. (2022) · Frontiers in Pharmacology · doi:10.3389/fphar.2022.962718 · Cited by 16 · see citing works
The morphology, functionality, and longevity of a novel all human hepatic cell-based tri-culture system
Weaver, J. R., Odanga, J. J., Wolf, K., Piekos, S. C., Biven, M., Taub, M. E., LaRocca, J. L., Thomas, C. T., Byer-Alcorace, A., Chen, J., et al. (2022) · Toxicology in Vitro · doi:10.1016/j.tiv.2022.105504 · Cited by 15 · see citing works
Microscale tissue engineering of liver lobule models: advancements and applications
Wang, Q., Liu, J., Yin, W., Wang, A., Zheng, J., Wang, Y., & Dong, J. (2023) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2023.1303053 · Cited by 15 · see citing works
Ex Vivo Tools and Models in MASLD Research
Velliou, R.-I., Giannousi, E., Ralliou, C., Kassi, E., & Chatzigeorgiou, A. (2024) · Cells · doi:10.3390/cells13221827 · Cited by 15 · see citing works
HepG2 as promising cell‐based model for biosynthesis of long‐term metabolites: Exemplified for metandienone
Zschiesche, A., Chundela, Z., Thieme, D., & Keiler, A. M. (2021) · Drug Testing and Analysis · doi:10.1002/dta.3184 · Cited by 14 · see citing works
Combining human liver ECM with topographically featured electrospun scaffolds for engineering hepatic microenvironment
Gao, Y., Gadd, V. L., Heim, M., Grant, R., Bate, T. S. R., Esser, H., Ferreira-González, S., Man, T. Y., Forbes, S. J., & Callanan, A. (2024) · Scientific Reports · doi:10.1038/s41598-024-73827-5 · Cited by 14 · see citing works
Metabolomic profiling during the differentiation of human induced pluripotent stem cells into hepatocyte-like cells
Jellali, R., Lereau, M., Tauran, Y., Gilard, F., Danoy, M., Kido, T., Miyajima, A., Sakai, Y., & Leclerc, É. (2019) · Differentiation · doi:10.1016/j.diff.2019.10.006 · Cited by 14 · see citing works
Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells
Lee, M., Lee, S., Jung, C., Son, Y. S., Ryu, J.-W., Jung, K. B., Ahn, J., Oh, J., Lee, H., Lim, J. H., et al. (2021) · Nature Communications · doi:10.1038/s41467-021-24746-w · Cited by 14 · see citing works
A Quantitative HILIC–MS/MS Assay of the Metabolic Response of Huh-7 Cells Exposed to 2,3,7,8-Tetrachlorodibenzo-p-Dioxin
Liu, Q., Cai, J., Nichols, R. G., Tian, Y., Zhang, J., Smith, P. B. W., Wang, Y., Yan, C., & Patterson, A. D. (2019) · Metabolites · doi:10.3390/metabo9060118 · Cited by 14 · see citing works

The human liver in vitro model: Potential of primary human hepatocytes for experimental and clinical research
Thasler, W., Weiß, T. S., Letschert, K., Tausch, U., Jilg, W., Hellerbrand, C., & Jauch, K. W. (2006) · University of Regensburg Publication Server (University of Regensburg)
Advances in generating liver cells from pluripotent stem cells as a tool for modeling liver diseases
Maepa, W., & Ndlovu, H. (2020) · Stem Cells · doi:10.1002/stem.3154 · Cited by 29 · see citing works
Development of a Novel Hepatocyte Model Using Stem Cells for Pharmaceutical Research
Ueyama-Toba, Y. (2023) · YAKUGAKU ZASSHI · doi:10.1248/yakushi.23-00130
Primary hepatocyte cultures for liver disease modeling
Vinken, M. (2020) · Current Opinion in Toxicology · doi:10.1016/j.cotox.2020.08.003 · Cited by 14 · see citing works
Human Three-Dimensional Hepatic Models: Cell Type Variety and Corresponding Applications
Xu, Q. (2021) · Frontiers in Bioengineering and Biotechnology · doi:10.3389/fbioe.2021.730008 · Cited by 36 · see citing works

Authors: Zeilinger, K., Freyer, N., Damm, G., Seehofer, D., & Knöspel, F.
Affiliations: Berlin-Brandenburger Centrum für Regenerative Therapien (DE)
Stiftung Charité (DE)
Corresponding author: Zeilinger, Katrin
ORCID: Zeilinger, Katrin
Damm, Georg
Seehofer, Daniel
Knöspel, Fanny
Venue: Experimental Biology and Medicine
Publisher: Frontiers Media SA
Published: 2016-07-24
Volume: 241
Issue: 15
Pages: 1684-1698
ISSN: 0071-3384 (ISSN-L)
1535-3702
1535-3699
DOI: 10.1177/1535370216657448
PMC: PMC4999620
OpenAlex: W2473248288
Cited by: 230
References: 158
Citations by year: 2026: 15 · 2025: 23 · 2024: 24 · 2023: 29 · 2022: 36 · 2021: 39 · 2020: 25 · 2019: 20 · 2018: 14 · 2017: 5
FWCI: 16.34
Type: journal article
Language: en
Open access: green
www.ncbi.nlm.nih.gov
Licence: journals.sagepub.com/page/policies/text-and-data-mining-license (tdm)
Locations: Experimental Biology and Medicine (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Bundesministerium für Bildung und Forschung (0315741)
Topics: Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Drug-Induced Hepatotoxicity and Protection
Health Sciences › Medicine › Hepatology › Liver physiology and pathology
Physical Sciences › Engineering › Biomedical Engineering › 3D Printing in Biomedical Research
Keywords: primary human hepatocytes, liver cell lines, pluripotent stem cells, hepatocyte-like cells, monolayer culture, hepatocyte differentiation, disease modeling, drug metabolism, toxicology
MeSH terms: Cell Line, Cells, Cultured, Humans, Liver, Stem Cells, Cell Culture Techniques, Hepatocytes, In Vitro Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Cell sources for in vitro human liver cell culture models

Abstract

Integrity signals

Claims the paper makes

Claims other papers make about this one

Self-check

Cites

Cited by

Related

Record

About these checks