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Cited by 61Nitric Oxide and Endothelin EffectsCardiovascular and exercise physiologyExercise and Physiological Responses

ROS and NO Dynamics in Endothelial Cells Exposed to Exercise-Induced Wall Shear Stress

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Wang, Yan-Xia, Liu, Hai-Bin, Li, Peng-Song, Yuan, Wen-Xue, Liu, Bo, Liu, Shu-Tian, Qin, Kai-Rong·2019·Cellular and Molecular Bioengineering
DOI 10.1007/s12195-018-00557-wPMC PMC6816775
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Abstract

Introduction Intracellular reactive oxygen species (ROS) and nitric oxide (NO) levels are associated with vascular homeostasis and diseases. Exercise can modulate ROS and NO production through increasing frequency and magnitude of wall shear stress (WSS). However, the details of ROS and NO production in endothelial cells and their interplay under WSS induced by exercise at different intensities remain unclear. Methods In this study, we developed an in vitro multicomponent nonrectangular flow chamber system to simulate pulsatile WSS waveforms induced by moderate and high intensity exercise. Furthermore, the dynamic responses of ROS and NO in endothelial cells and the relationship between ROS and NO were investigated under the WSS induced by different intensity exercise. Results After exposing to WSS induced by moderate intensity exercise, endothelial cells produced more NO than those under high intensity exercise-induced WSS. In this process, ROS was found to play a dual role in the generation of intracellular NO. Under WSS induced by moderate intensity exercise, modest elevated ROS promoted NO production, whereas excessive ROS in endothelial cells exposed to WSS induced by high intensity exercise attenuated NO bioavailability. Interestingly, antioxidant N-acetylcysteine (NAC) could increase NO production under WSS induced by high intensity exercise. Conclusions Our results provide some cues for selecting appropriate exercise intensities and elevating benefits of exercise on endothelial function. Additionally, owing to the consistency of our results and some in vivo phenomena, this flow chamber system may serve as an in vitro exercise model of arterial vessel for future studies.

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  • Repeated high-intensity exercise may alter hemodynamics by increasing shear stress, particularly in proximal coronary segments, potentially promoting focal atherosclerosis.

    “First, repeated high-intensity exercise may alter hemodynamics by increasing shear stress, particularly in proximal coronary segments, potentially promoting focal atherosclerosis.12,29,30”

    From Wearable-Derived Training Load and Coronary Atherosclerosis in Middle-Aged and Older Athletes and Physically Active Controls: A New Perspective From the Master@Heart Study (2026) · cites this paper as [29]

    The Introduction and Discussion (pages unavailable) state that exercise increases the magnitude and frequency of wall shear stress, including under conditions modeled for high-intensity exercise. The paper does not establish that this occurs particularly in proximal coronary segments or that repeated high-intensity exercise promotes focal atherosclerosis; its experiments concern endothelial-cell ROS and NO responses in a flow chamber. (LLM, conf=0.88)

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Cited by

Sorted by citations

Authors
Wang, Y.-X., Liu, H.-B., Li, P.-S., Yuan, W.-X., Liu, B., Liu, S.-T., & Qin, K.-R.
Affiliations
Dalian University of Technology (CN)
Corresponding author
Qin, Kai-Rong
ORCID
Venue
Cellular and Molecular Bioengineering
Publisher
Springer Science and Business Media LLC
Published
2018-10-08
Volume
12
Issue
1
Pages
107-120
ISSN
  • 1865-5025 (ISSN-L)
  • 1865-5033
DOI
10.1007/s12195-018-00557-w
PMC
PMC6816775
OpenAlex
W2897690825
Cited by
61
References
2
Citations by year
2026: 11 · 2025: 11 · 2024: 13 · 2023: 8 · 2022: 8 · 2021: 8 · 2020: 2
FWCI
1.72
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • Cellular and Molecular Bioengineering (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Natural Science Foundation of China (31370948, 11672065)
  • Fundamental Research Funds for the Central Universities in China (DUT18JC15)
Topics
  • Health Sciences › Medicine › Physiology › Nitric Oxide and Endothelin Effects
  • Health Sciences › Medicine › Complementary and alternative medicine › Cardiovascular and exercise physiology
  • Health Sciences › Medicine › Rehabilitation › Exercise and Physiological Responses
Keywords
reactive oxygen species, nitric oxide, endothelial cells, wall shear stress, exercise intensity, pulsatile flow, NO bioavailability, N-acetylcysteine, antioxidants, vascular homeostasis

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