CoChat
Sign Up
Cited by 16

The DOI matches its Crossref record.

High fluoride resistance and virulence profile of environmental Pseudomonas isolated from water sources

Chellaiah, Edward Raja, Ravi, Pandeeswari, Uthandakalaipandian, Ramesh·2021·Folia Microbiologica
DOI 10.1007/s12223-021-00867-z
Open this paper in CoChat — free account

Abstract

In our previous study, all Pseudomonas strains THP6, THP41, and OHP5 were identified as fluoride-resistant bacteria isolated from Dindigul district, Tamilnadu, India. The selected strains exhibiting a high level of fluoride resistance was determined in Luria broth (LB) medium and LB agar plates. In a further effort, fluoride-resistant organisms were tested for hemolytic activity and showed β-hemolysis on blood agar plates. The virulence factors such as gyrB, toxA, algD and lasB, plcH, rhlC and biofilm response genes (pslA, pelA, ppyR) were detected by PCR analysis. The putative genus-specific and species-specific PCR also confirmed that the selected fluoride-resistant strains were belonging to Pseudomonas aeruginosa species. Fluoride-resistance gene crcB was amplified by gene-specific primers. The crcB gene was cloned in TA vector and transformed into E. coli DH5α. Comparative and blast analysis of THP6, THP41, and OHP5 strains crcB gene sequences were high homology with P. aeruginosa fluoride efflux transporter crcB and P. aeruginosa putative fluoride ion transporter crcB. The recombinants were efficiently growing in the NaF containing LB agar plates. The fluoride tolerance of these strains was also associated with resistance to multiple antibiotics. These results can lead to the use of the fluoride resistance gene of P. aeruginosa for the development of a biosensor for fluoride detection.

Overview

Integrity signals

Registry

Crossref · 10.1007/s12223-021-00867-z

Cites

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • Earlier in the authors' research group, Pseudomonas species were identified as fluoride resistant bacteria isolated from Thadikombu and Ottupatti villages at Dindigul district, Tamil Nadu.

    “Earlier in my research group identified Pseudomonas species are fluoride resistant bacteria isolated from Thadikombu and Ottupatti villages at Dindigul district, Tamil Nadu (Kirupa Sree et al. 2018; Edward Raja et al. 2021).”

    From Isolation and identification of high fluoride resistant bacteria from water samples of Dindigul district, Tamil Nadu, South India (2021) · cites this paper as Edward Raja et al. 2021

Citations

Cites

No references could be resolved yet.

Research works printed but not linked: 48

  1. Abada E, Al-Fifi Z, Al-Rajab AJ, Mahdhi M, Sharma M (2019) Molecular identification of biological contaminants in different drinking water resources of the Jazan region, Saud Arabia. J Water Health 17:622–632. https://doi.org/10.2166/wh.2019.019 · DOI
  2. Adimalla N, Venkatayogi S (2017) Mechanism of fluoride enrichment in groundwater of hard rock aquifers in Medak, Telangana State, South India. Environ Earth Sci 76:45. https://doi.org/10.1007/s12665-016-6362-2 · DOI
  3. Abdel-Mawgoud AM, Lepine F, Deziel E (2010) Rhamnolipids: diversity of structures, microbial origins and roles. Appl Microbiol Biotechnol 86:1323–1336. https://doi.org/10.1007/s00253-010-2498-2 · DOI
  4. Altschul SF, Maddan TL, Schaffer AA, Zang J, Zang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSIBLAST: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402. https://doi.org/10.1093/nar/25.17.3389 · DOI · Paper page
  5. Baker JL, Sudarsan L, Weinberg Z, Roth A, Stockbridge RB, Breaker RR (2012) Widespread genetic switches and toxicity resistance proteins for fluoride. Science 335:233-235. https://doi.org/10.1126/science.1215063 · DOI · Paper page
  6. Balasubramanian D, Schneper L, Kumari H, Mathee K (2013) A dynamic and intricate regulatory network determines Pseudomonas aeruginosa virulence. Nucleic Acids Res 41:1–20. https://doi.org/10.1093/nar/gks1039 · DOI
  7. Bej AK, Mahbubani MH, Dicesare JL, Atlas RM (1991) Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples. Appl Environ Microbiol 57:3529–3534 · DOI
  8. Edrington TS, Fox WE, Callaway TR, Anderson RC, Hoffman DW, Nisbet DJ (2009) Pathogen prevalence and influence of composted dairy manure application on antimicrobial resistance profiles of commensal soil bacteria. Foodborne Pathog Dis 6:217–224. https://doi.org/10.1089/fpd.2008.0184 · DOI · Paper page
  9. Edward Raja C (2018) Cadmium (heavy metals) bioremediation by Pseudomonas aeruginosa: a minireview. Appl Water Sci 8:154. https://doi.org/10.1007/s13201-018-0796-5 · DOI
  10. Edward Raja C, Anbazhagan K, Selvam GS (2006) Isolation and characterization of a metal resistant Pseudomonas aeruginosa strain. World J Microbiol Biotechnol 22:577–585. https://doi.org/10.1007/s11274-005-9074-4 · DOI
  11. Eren E, Ozturk M, Mumcu EF, Canatan D (2005) Fluorosis and its hematological effects. Toxicol Ind Health 21:255-258. https://doi.org/10.1191/0748233705th236oa · DOI · Paper page
  12. Fields PI, Popovic T, Wachsmuth K, Olsvik O (1992) Use of polymerase chain reaction for the detection of toxigenic Vibrio cholerae 01 strains from the Latin American cholera epidemic. J Clin Microbiol 30:2118–2121 · DOI
  13. Hancock RE, Speert DP (2000) Antibiotic resistance in Pseudomonas aeruginosa: mechanisms and impact on treatment. Drug Resist Updat 3:247–255. https://doi.org/10.1054/drup.2000.0152 · DOI
  14. Hashish NMA, Abbass AAG, Amine AEK (2017) Pseudomonas aeruginosa in swimming pools. Cogent Environ Sci 3:1328841 · DOI
  15. Hassan A, Usman J, Kaleem F, Omair M, Khalid A, Iqbal M (2011) Evaluation of different detection methods of biofilm formation in the clinical isolates. Braz J Infect Dis 15:305–311. https://doi.org/10.1016/S1413-8670(11)70197-0 · DOI
  16. Hassan SHA, Abskharon RN, Gad El-Rab SMF, Shoreit AAM (2008) Isolation, characterization of heavy metal resistant strain of Pseudomonas aeruginosa isolated from polluted sites in Assiut city. Egypt. J Basic Microbiol 48:168–176. https://doi.org/10.1002/jobm.200700338 · DOI
  17. Hou W, Sun X, Wang Z, Zhang Y (2012) Biofilm-forming capacity of Staphylococcus epidermidis, Staphylococcus aureus, and Pseudomonas aeruginosa from ocular infections biofilm-forming capacity of human flora bacteria. Invest Ophthalmol Vis Sci 53:5624–5631. https://doi.org/10.1167/iovs.11-9114 · DOI
  18. Karatuna O, Yagci A (2010) Analysis of quorum sensing dependent virulence factor production and its relationship with antimicrobial susceptibility in Pseudomonas aeruginosa respiratory isolates. Clin Microbiol Infect 16:1770–1775. https://doi.org/10.1111/j.1469-0691.2010.03177.x · DOI
  19. Katiyar P, Pandey N, Sahu KK (2020) Biological approaches of fluoride remediation: potential for environmental clean-up. Environ Sci Pollut Res 27:13044-13055. https://doi.org/10.1007/s11356-020.-08224-2 · DOI
  20. Khan AA, Cerniglia CE (1994) Detection of Pseudomonas aeruginosa from clinical and environmental samples by amplification of the exotoxin A gene using PCR. Appl Environ Microbiol 60:3739–3745 · DOI
  21. Lambert PA (2002) Mechanisms of antibiotic resistance in Pseudomonas aeruginosa. J Roy Soc Med 95:22–26
  22. Lanotte P, Watt S, Mereghetti L, Dartiguelongue N, Rastegar-Lari A, Goudeau A, Quentin R (2004) Genetic features of Pseudomonas aeruginosa isolates from cystic fibrosis patients compared with those of isolates from other origins. J Med Microbiol 53:73–81. https://doi.org/10.1099/jmm.0.05324-0 · DOI
  23. Laverty G, Gorman SP, Gilmore BF (2014) Biomolecular mechanisms of Pseudomonas aeruginosa and Escherichia coli biofilm formation. Pathogens 3:596–632. https://doi.org/10.3390/pathogens3030596 · DOI
  24. Lee CS, Wetzel K, Buckley T, Wozniak D, Lee J (2011) Rapid and sensitive detection of Pseudomonas aeruginosa in chlorinated water and aerosols targeting gyrB gene using real-time PCR. J Appl Microbiol 111:893–903. https://doi.org/10.1111/j.1365-2672.2011.05107.x · DOI
  25. Ma KY, Sun MY, Dong W, He CQ, Chen FL, Ma YL (2016) Effects of nutrition optimization strategy on rhamnolipid production in a Pseudomonas aeruginosa strain DN1 for bioremediation of crude oil. Biocatal Agric Biotechnol 6:144-151.https://doi.org/10.1016/j.bcab.2016.03.008 · DOI
  26. Majtán V, Hostacká A, Kosiarová A, Majtánová L, Kohutová S (1991) On the toxinogenicity of Pseudomonas aeruginosa strains. J Hyg Epidemiol Microbiol Immunol 35:217–224
  27. Martins VV, Pitondo-Silva A, Manco Lde M, Falcao JP, Freitas SS, da Silveira WD, Stehling EG (2013) Pathogenic potential and genetic diversity of environmental and clinical isolates of Pseudomonas aeruginosa. APMIS 122:92–100. https://doi.org/10.1111/apm.12112 · DOI
  28. Michalska M, Wolf P (2015) Pseudomonas exotoxin A: optimized by evolution for effective killing. Front Microbiol 6:963. https://doi.org/10.3389/fmicb.2015.00963 · DOI
  29. Mosharaf MK, Tanvir MZH, Haque MM, Haque MA, Khan MAA, Molla AH, Alam MZ, Islam MS, Talukder MR (2018) Metal-adapted bacteria isolated from wastewaters produce biofilms by expressing proteinaceous curli fimbriae and cellulose nanofibers. Front Microbiol 9:1334. https://doi.org/10.3389/fmicb.2018.01334 · DOI
  30. Najafi K, Kafil HS, Shokrian S, Azimi S, Asgharzadeh M, Yousefi M, Aghazadeh M (2015) Virulence genes and antibiotic resistance profile of Pseudomonas aeruginosa isolates in Northwest of Iran. J Pure Appl Microbiol 9:383–389
  31. Nguyen CC, Hugie CN, Kile ML, Daneshmand TN (2019) Association between heavy metals and antibiotic-resistant human pathogens in environmental reservoirs: a review. Front Environ Sci Eng 13(3):46. https://doi.org/10.1007/s11783-019-1129-0 · DOI
  32. Pitondo-Silva A, Goncalves GB, Stehling EG (2016) Heavy metal resistance and virulence profile in Pseudomonas aeruginosa isolated from Brazilian soils. APMIS 124:681–688. https://doi.org/10.1111/apm.12553 · DOI
  33. Pournajaf A, Razavi S, Irajian G, Ardebili A, Erfani Y, Solgi S, Yaghoubi S, Rasaeian A, Yahyapour Y, Kafshgari R, Shoja S, Rajabnia R (2018) Integron types, antimicrobial resistance genes, virulence gene profile, alginate production and biofilm formation in Iranian cystic fibrosis Pseudomonas aeruginosa isolates. Infez Med 26:226–236
  34. Qin X, Emerson J, Stapp J, Stapp L, Abe P, Burns JL (2003) Use of real-time PCR with multiple targets to identify Pseudomonas aeruginosa and other nonfermenting gram-negative bacilli from patients with cystic fibrosis. J Clin Microbiol 41:4312–4317. https://doi.org/10.1128/JCM.41.9.4312-4317.2003 · DOI
  35. Rahim R, Ochsner UA, Olvera C, Graninger M, Messner P, Lam JS, Soberoan-Chaavez G (2001) Cloning and functional characterization of the Pseudomonas aeruginosa rhlC gene that encodes rhamnosyltransferase 2, an enzyme responsible for di-rhamnolipid biosynthesis. Mol Microbiol 40:708–718. https://doi.org/10.1046/j.1365-2958.2001.02420.x · DOI
  36. Reis RS, Pereira AG, Neves BC, Freire DM (2011) Gene regulation of rhamnolipid production in Pseudomonas aeruginosa - a review. Bioresour Technol 102:6377-6384. https://doi.org/10.1016/j.biortech.2011.03.074 · DOI
  37. Rocha AJ, Barsottini MRDO, Laurindo MV, Moraes FLLD, Rocha SLD (2019) Pseudomonas aeruginosa: virulence factors and antibiotic resistance genes. Braz Arch Biol Technol 62:e19180503. https://doi.org/10.1590/1678-4324-2019180503 · DOI
  38. Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer directed enzymatic amplification of DNA with thermostable DNA polymerase. Science 239:487–491. https://doi.org/10.1126/science.239.4839.487 · DOI · Paper page
  39. Schwartz T, Volkmann H, Kirchen S, Kohnen W, Schon-Holz K, Jansen B, Obst U (2006) Real-time PCR detection of Pseudomonas aeruginosa in clinical and municipal wastewater and genotyping of the ciprofloxacin-resistant isolates. FEMS Microbiol Ecol 57:158–167. https://doi.org/10.1111/j.1574-6941.2006.00100.x · DOI
  40. Silby MW, Winstanley C, Godfrey SA, Levy SB, Jackson RW (2011) Pseudomonas genomes: diverse and adaptable. FEMS Microbiol Rev 35:652–680. https://doi.org/10.1111/j.1574-6976.2011.00269.x · DOI
  41. Skariyachan S, Sridhar VS, Packirisamy S, Kumargowda ST, Challapilli SB (2018) Recent perspectives on the molecular basis of biofilm formation by Pseudomonas aeruginosa and approaches for treatment and biofilm dispersal. Folia Microbiol 63:413–432. https://doi.org/10.1007/s12223-018-0585-4 · DOI
  42. Spilker T, Coenye T, Vandamme P, LiPuma JJ (2004) PCR-based assay for differentiation of Pseudomonas aeruginosa from other Pseudomonas species recovered from cystic fibrosis patients. J Clin Microbiol 42:2074–2079. https://doi.org/10.1128/jcm.42.5.2074-2079.2004 · DOI
  43. Stepanovic S, Vukovic D, Dakic I, Savic B, Svabic-Vlahovic M (2000) A modified microtiter-plate test for quantification of staphylococcal biofilm formation. J Microbiol Methods 40:175–179. https://doi.org/10.1016/S0167-7012(00)00122-6 · DOI
  44. Stockbridge RB, Lim HH, Otten R, Williams C, Shane T, Weinberg Z, Miller C (2012) Fluoride resistance and transport by riboswitch-controlled CLC antiporters. Proc Natl Acad Sci U S A 109:15289–15294 · DOI · Paper page
  45. Tekpor M, Akrong M, Asmah MH, Banu RA, Ansa EDO (2017) Bacteriological quality of drinking water in the Atebubu-Amantin district of the Brong-Ahafo region of Ghana. Appl Water Sci 7:2571–2576. https://doi.org/10.1007/s13201-016-0457-5 · DOI
  46. Wagner VE, Li LL, Isabella VM, Iglewski BH (2007) Analysis of the hierarchy of quorum-sensing regulation in Pseudomonas aeruginosa. Anal Bioanal Chem 387:469–479. https://doi.org/10.1007/s00216-006-0964-6 · DOI
  47. Wretlind B, Sjoberg L, Wadstrom T (1977) Protease-deficient mutants of Pseudomonas aeruginosa: pleiotropic changes in activity of other extracellular enzymes. J Gen Microbiol 103:329-336. https://doi.org/10.1099/00221287-103-2-329 · DOI
  48. Wunderink RG, Mendoza DL (2007) Epidemiology of Pseudomonas aeruginosa in the intensive care unit. In: Rello J, Kollef M, Díaz E, Rodríguez A (eds) Infectious diseases in critical care. Springer-Verlag, Berlin Heidelberg, pp 218–225 · DOI

Cited by

Sorted by citations

Metadata

Authors
Chellaiah, E. R., Ravi, P., & Uthandakalaipandian, R.
ORCID
Chellaiah, Edward Raja
Venue
Folia Microbiologica
Publisher
Springer Science and Business Media LLC
Published
2021-04-05
Volume
66
Issue
4
Pages
569-578
ISSN
  • 0015-5632
  • 1874-9356
DOI
10.1007/s12223-021-00867-z
Cited by
16
References
0
Language
en
Licence

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review