Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

Saiki, Randall K.; Gelfand, David H.; Stoffel, Susanne; Scharf, Stephen J.; Higuchi, Russell; Horn, Glenn T.; Mullis, Kary B.; Erlich, Henry A.

doi:10.1126/science.2448875

Cited by 17,719Molecular Biology Techniques and ApplicationsDNA and Nucleic Acid ChemistryGenomics and Phylogenetic Studies

The DOI matches its Crossref record; 19 of the 19 references it cites were found in a registry or an index.

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

Saiki, Randall K., Gelfand, David H., Stoffel, Susanne, Scharf, Stephen J., Higuchi, Russell, Horn, Glenn T., Mullis, Kary B., Erlich, Henry A.·1988·Science

DOI 10.1126/science.2448875

Open this paper in CoChat — free account

Abstract

A thermostable DNA polymerase was used in an in vitro DNA amplification procedure, the polymerase chain reaction. The enzyme, isolated from Thermus aquaticus , greatly simplifies the procedure and, by enabling the amplification reaction to be performed at higher temperatures, significantly improves the specificity, yield, sensitivity, and length of products that can be amplified. Single-copy genomic sequences were amplified by a factor of more than 10 million with very high specificity, and DNA segments up to 2000 base pairs were readily amplified. In addition, the method was used to amplify and detect a target DNA molecule present only once in a sample of 10 5 cells.

Integrity signals

Registry

Crossref · 10.1126/science.2448875

Cites

19 of 19 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Molecular Cloning: A Laboratory Manual
Sambrook, J., Fritsch, E., & Maniatis, T. (1989) · UCLA - Biblioteca de Ciencias de la Salud · Cited by 133,589
DNA sequencing with chain-terminating inhibitors
Sanger, F., Nicklen, S., & Coulson, A. R. (1977) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.74.12.5463 · Cited by 68,572
[57] Sequencing end-labeled DNA with base-specific chemical cleavages
Maxam, A. M., & Gilbert, W. (1980) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/s0076-6879(80)65059-9 · Cited by 14,678
Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell Anemia
Saiki, R. K., Scharf, S., Faloona, F., Mullis, K. B., Horn, G. T., Erlich, H. A., & Arnheim, N. (1985) · Science · doi:10.1126/science.2999980 · Cited by 8,956
Self-citation
[21] Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction
Mullis, K. B., & Faloona, F. (1987) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(87)55023-6 · Cited by 5,705
Self-citation
Prevalence of ras gene mutations in human colorectal cancers
Bos, J. L., Fearon, E. R., Hamilton, S. R., Vries, M. V., BOOM, J. H. V., van der Eb, A. J., & Vogelstein, B. (1987) · Nature · doi:10.1038/327293a0 · Cited by 1,860
Analysis of enzymatically amplified β-globin and HLA-DQα DNA with allele-specific oligonucleotide probes
Saiki, R. K., Bugawan, T. L., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1986) · Nature · doi:10.1038/324163a0 · Cited by 1,826
Self-citation
Rapid transfer of DNA from agarose gels to nylon membranes
Reed, K. C., & Mann, D. A. (1985) · Nucleic Acids Research · doi:10.1093/nar/13.20.7207 · Cited by 1,731
Direct Cloning and Sequence Analysis of Enzymatically Amplified Genomic Sequences
Scharf, S. J., Horn, G. T., & Erlich, H. A. (1986) · Science · doi:10.1126/science.3461561 · Cited by 747
Self-citation
Deoxyribonucleic acid polymerase from the extreme thermophile Thermus aquaticus
Chien, A. J., Edgar, D., & Trela, J. M. (1976) · Journal of Bacteriology · doi:10.1128/jb.127.3.1550-1557.1976 · Cited by 699
Characterization of β-thalassaemia mutations using direct genomic sequencing of amplified single copy DNA
Wong, C., Dowling, C. E., Saiki, R. K., et al. (1987) · Nature · doi:10.1038/330384a0 · Cited by 505
Self-citation
Detection of Minimal Residual Cells Carrying the t(14;18) by DNA Sequence Amplification
Lee, M.-S., Chang, K.-S., Cabanillas, F., et al. (1987) · Science · doi:10.1126/science.3110950 · Cited by 421
Alternative male life histories in bluegill sunfish
Gross, M. R., & Charnov, E. L. (1980) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.77.11.6937 · Cited by 397
Length mutations in human mitochondrial DNA: direct sequencing of enzymatically amplified DNA
Wrischnik, L. A., Higuchi, R., Stoneking, M., Erlich, H. A., Arnheim, N., & Wilson, A. C. (1987) · Nucleic Acids Research · doi:10.1093/nar/15.2.529 · Cited by 374
Self-citation
Identification of human immunodeficiency virus sequences by using in vitro enzymatic amplification and oligomer cleavage detection
Kwok, S., Mack, D. H., Mullis, K. B., Poiesz, B. J., Ehrlich, G. D., Blair, D. C., Friedman‐Kien, A. E., & Sninsky, J. J. (1987) · Journal of Virology · doi:10.1128/jvi.61.5.1690-1694.1987 · Cited by 333
Self-citation
Characterization of c-Ki-ras oncogene alleles by direct sequencing of enzymatically amplified DNA from carcinogen-induced tumors.
McMahon, G., Davis, E., & Wogan, G. N. (1987) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.84.14.4974 · Cited by 131
Rapid Prenatal Diagnosis of Sickle Cell Anemia by a New Method of DNA Analysis
Embury, S. H., Scharf, S. J., Saiki, R. K., Gholson, M. A., Golbus, M. S., Arnheim, N., & Erlich, H. A. (1987) · New England Journal of Medicine · doi:10.1056/nejm198703123161103 · Cited by 128
Self-citation
Nucleotide sequence coding for the “signal peptide” and N terminus of the hemagglutinin from an Asian (H2N2) strain of influenza virus
Air, G. M. (1979) · Virology · doi:10.1016/0042-6822(79)90358-1 · Cited by 127
Different 3' end points of deletions causing delta beta-thalassemia and hereditary persistence of fetal hemoglobin: implications for the control of gamma-globin gene expression in man.
Tuan, D. Y. H., Feingold, E., Newman, M., Weissman, S., & Forget, B. G. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.22.6937 · Cited by 116

Flagged first, then by citations

Research works printed but not linked: 1

Tuan, D., Proceedings of the National Academy of Sciences of the United States of America 80: 6937 (1983).

Sorted by citations

Loop-mediated isothermal amplification of DNA
Notomi, T. (2000) · Nucleic Acids Research · doi:10.1093/nar/28.12.e63 · Cited by 8,805
Identification of the Cystic Fibrosis Gene: Genetic Analysis
Kerem, B.-S., Rommens, J. M., Buchanan, J. A., Markiewicz, D., Cox, T. K., Chakravarti, A., Buchwald, M., & Tsui, L.-C. (1989) · Science · doi:10.1126/science.2570460 · Cited by 4,142
Activation of a member of the steroid hormone receptor superfamily by peroxisome proliferators
Issemann, I., & Green, S. (1990) · Nature · doi:10.1038/347645a0 · Cited by 3,519
Presence of fetal DNA in maternal plasma and serum
Lo, Y. M. D., Corbetta, N., Chamberlain, P. F., Rai, V., Sargent, I. L., Redman, C. W., & Wainscoat, J. S. (1997) · The Lancet · doi:10.1016/s0140-6736(97)02174-0 · Cited by 3,129
A mutation in the tRNALeu(UUR) gene associated with the MELAS subgroup of mitochondrial encephalomyopathies
Goto, Y.-I., Nonaka, I., & Horai, S. (1990) · Nature · doi:10.1038/348651a0 · Cited by 2,070
Towards next-generation biodiversity assessment using DNA metabarcoding
TABERLET, P., COISSAC, E., POMPANON, F., BROCHMANN, C., & WILLERSLEV, E. (2012) · Molecular Ecology · doi:10.1111/j.1365-294x.2012.05470.x · Cited by 1,907
The murine mutation osteopetrosis is in the coding region of the macrophage colony stimulating factor gene
Yoshida, H., Hayashi, S.-I., Kunisada, T., Ogawa, M., Nishikawa, S., Okamura, H., Sudo, T., Shultz, L. D., & Nishikawa, S.-I. (1990) · Nature · doi:10.1038/345442a0 · Cited by 1,789
Reliable genotyping of samples with very low DNA quantities using PCR
Taberlet, P. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.16.3189 · Cited by 1,491
Comprehensive human genome amplification using multiple displacement amplification
Dean, F. B., Hosono, S., Fang, L., Wu, X., Faruqi, A. F., Bray-Ward, P., Sun, Z., Zong, Q., Du, Y., Du, J., et al. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.082089499 · Cited by 1,467
The antioxidant responsive element. Activation by oxidative stress and identification of the DNA consensus sequence required for functional activity
Rushmore, T. H., Morton, M. R., & Pickett, C. B. (1991) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)99004-6 · Cited by 1,464
The sequence of sequencers: The history of sequencing DNA
Heather, J. M., & Chain, B. (2016) · Genomics · doi:10.1016/j.ygeno.2015.11.003 · Cited by 1,397
Clinical and genetic features of Ehlers-Danlos syndrome type IV, the vascular type
Pepin, M., Schwarze, U., Superti-Furga, A., & Byers, P. H. (2000) · New England Journal of Medicine · doi:10.1056/nejm200003093421001 · Cited by 1,344
Genetic analyses from ancient DNA
Pääbo, S., Poinar, H., Serre, D., Jaenicke-Després, V., Hebler, J., Rohland, N., Kuch, M., Krause, J., Vigilant, L., & Hofreiter, M. (2004) · Annual Review of Genetics · doi:10.1146/annurev.genet.37.110801.143214 · Cited by 1,333
Neurotrophin-3: a Neurotrophic Factor Related to NGF and BDNF
Maisonpierre, P. C., Belluscio, L., Squinto, S., Ip, N. Y., Furth, M. E., Lindsay, R. M., & Yancopoulos, G. D. (1990) · Science · doi:10.1126/science.2321006 · Cited by 1,322
The purification, cloning and expression of a novel luteinizing hormone-induced mitochondrial protein in MA-10 mouse Leydig tumor cells. Characterization of the steroidogenic acute regulatory protein (StAR)
Clark, B. J., Wells, J., King, S. R., & Stocco, D. M. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)46930-x · Cited by 1,168
Regulation of the acute production of steroids in steroidogenic cells
STOCCO, D. M., & CLARK, B. J. (1996) · Endocrine Reviews · doi:10.1210/edrv-17-3-221 · Cited by 1,057
Analysis of aptamer discovery and technology
Dunn, M. R., Jimenez, R. M., & Chaput, J. C. (2017) · Nature Reviews Chemistry · doi:10.1038/s41570-017-0076 · Cited by 938
The Agent of Bacillary Angiomatosis: An Approach to the Identification of Uncultured Pathogens
Relman, D. A., Loutit, J. S., Schmidt, T. M., Falkow, S., & Tompkins, L. S. (1990) · New England Journal of Medicine · doi:10.1056/nejm199012063232301 · Cited by 901
The mouse peroxisome proliferator activated receptor recognizes a response element in the 5′ flanking sequence of the rat acyl CoA oxidase gene
Tugwood, J. D., Issemann, I., Anderson, R. G., Bundell, K. R., McPheat, W. L., & Green, S. (1992) · The EMBO Journal · doi:10.1002/j.1460-2075.1992.tb05072.x · Cited by 895
BDNF mRNA expression is increased in adult rat forebrain after limbic seizures: Temporal patterns of induction distinct from NGF
Isackson, P. J., Huntsman, M. M., Murray, K. D., & Gall, C. M. (1991) · Neuron · doi:10.1016/0896-6273(91)90234-q · Cited by 711
Deletion of steroid 5α-reductase 2 gene in male pseudohermaphroditism
Andersson, S., Berman, D. M., Jenkins, E. P., & Russell, D. W. (1991) · Nature · doi:10.1038/354159a0 · Cited by 643
Human hypertension caused by mutations in the kidney isozyme of 11β–hydroxysteroid dehydrogenase
Mune, T., Rogerson, F. M., Nikkilä, H., Agarwal, A. K., & White, P. C. (1995) · Nature Genetics · doi:10.1038/ng0895-394 · Cited by 619
A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells
Ikuta, K., Kina, T., MacNeil, I., Uchida, N., Peault, B., Chien, Y.-H., & Weissman, I. L. (1990) · Cell · doi:10.1016/0092-8674(90)90262-d · Cited by 583
Cloning and characterization of a vasopressin V2 receptor and possible link to nephrogenic diabetes insipidus
Lolait, S. J., O'Carroll, A.-M., McBride, O. W., Konig, M., Morel, A., & Brownstein, M. J. (1992) · Nature · doi:10.1038/357336a0 · Cited by 524
Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies
Lorenz, T. C. (2012) · Journal of Visualized Experiments · doi:10.3791/3998 · Cited by 493
Autoimmunity to two forms of glutamate decarboxylase in insulin-dependent diabetes mellitus
Kaufman, D. L., Erlander, M. G., Clare-Salzler, M., Atkinson, M. A., Maclaren, N. K., & Tobin, A. J. (1992) · Journal of Clinical Investigation · doi:10.1172/jci115573 · Cited by 490
Tissue-specific expression of the human receptor for glucagon-like peptide-I: Brain, heart and pancreatic forms have the same deduced amino acid sequences
Wei, Y., & Mojsov, S. (1995) · FEBS Letters · doi:10.1016/0014-5793(94)01430-9 · Cited by 450
Biochemical Basis of DNA Replication Fidelity
Goodman, M. F., Creighton, S., Bloom, L. B., Petruska, J., & Kunkel, T. A. (1993) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409239309086792 · Cited by 446
Molecular genetics of steroid 5 alpha-reductase 2 deficiency
Thigpen, A. E., Davis, D. L., Milatovich, A., Mendonca, B. B., Imperato-McGinley, J., Griffin, J. E., Francke, U., Wilson, J. D., & Russell, D. W. (1992) · Journal of Clinical Investigation · doi:10.1172/jci115954 · Cited by 422
A β 3 Integrin Mutation Abolishes Ligand Binding and Alters Divalent Cation-Dependent Conformation
Loftus, J. C., O'Toole, T. E., Plow, E. F., Glass, A., Frelinger, A. L., & Ginsberg, M. H. (1990) · Science · doi:10.1126/science.2392682 · Cited by 401
Genetic and pharmacological evidence for more than one human steroid 5 alpha-reductase.
Jenkins, E. P., Andersson, S., Imperato-McGinley, J., Wilson, J. D., & Russell, D. W. (1992) · Journal of Clinical Investigation · doi:10.1172/jci115574 · Cited by 395
Tissue distribution and kinetic characteristics of rat steroid 5 alpha-reductase isozymes. Evidence for distinct physiological functions.
Normington, K., & Russell, D. W. (1992) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)41809-1 · Cited by 379
Structural Changes Are Associated with Soluble N-Ethylmaleimide-sensitive Fusion Protein Attachment Protein Receptor Complex Formation
Fasshauer, D., Otto, H., Eliason, W. K., Jahn, R., & Brünger, A. T. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.44.28036 · Cited by 378
Point mutation causing a single amino acid substitution in the hormone binding domain of the glucocorticoid receptor in familial glucocorticoid resistance
Hurley, D. M., Accili, D., Stratakis, C. A., Karl, M., Vamvakopoulos, N., Rorer, E., Constantine, K., Taylor, S. I., & Chrousos, G. P. (1991) · Journal of Clinical Investigation · doi:10.1172/jci115046 · Cited by 370
Extension of base mispairs by Taq DNA polymerase: implications for single nucleotide discrimination in PCR
Huang, M.-M., Arnheim, N., & Goodman, M. F. (1992) · Nucleic Acids Research · doi:10.1093/nar/20.17.4567 · Cited by 365
Latent human herpesvirus 6 infection of human monocytes/macrophages
Kondo, K., Kondo, T., Okuno, T., Takahashi, M., & Yamanishi, K. (1991) · Journal of General Virology · doi:10.1099/0022-1317-72-6-1401 · Cited by 341
Genome-wide bioinformatic and molecular analysis of introns in Saccharomyces cerevisiae
SPINGOLA, M., GRATE, L., HAUSSLER, D., & ARES, M. (1999) · RNA · doi:10.1017/s1355838299981682 · Cited by 316
The heterochromatin-associated protein HP-1 is an essential protein in Drosophila with dosage-dependent effects on position-effect variegation.
Eissenberg, J. C., Morris, G. D., Reuter, G., & Hartnett, T. (1992) · Genetics · doi:10.1093/genetics/131.2.345 · Cited by 315
The Drive to Life on Wet and Icy Worlds
Russell, M. J., Barge, L. M., Bhartia, R., Bocanegra, D., Bracher, P. J., Branscomb, E., Kidd, R., McGlynn, S., Meier, D. H., Nitschke, W., et al. (2014) · Astrobiology · doi:10.1089/ast.2013.1110 · Cited by 288
Identification of mutations leading to the Lesch-Nyhan syndrome by automated direct DNA sequencing of in vitro amplified cDNA.
Gibbs, R. A., Nguyen, P. N., McBride, L. J., Koepf, S. M., & Caskey, C. T. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.6.1919 · Cited by 275
A mutation in CYP11B1 (Arg-448----His) associated with steroid 11 beta-hydroxylase deficiency in Jews of Moroccan origin.
White, P. C., Dupont, J., New, M. I., Leiberman, E., Hochberg, Z., & Rösler, A. (1991) · Journal of Clinical Investigation · doi:10.1172/jci115182 · Cited by 267
The Size of the Human Proteome: The Width and Depth
Ponomarenko, E. A., Poverennaya, E. V., Ilgisonis, E. V., Pyatnitskiy, M. A., Kopylov, A. T., Zgoda, V. G., Lisitsa, A. V., & Archakov, A. I. (2016) · International Journal of Analytical Chemistry · doi:10.1155/2016/7436849 · Cited by 267
Tissue-specific promoters regulate aromatase cytochrome P450 gene expression in human ovary and fetal tissues
Means, G. D., Kilgore, M. W., Mahendroo, M. S., Mendelson, C. R., & Simpson, E. R. (1991) · Molecular Endocrinology · doi:10.1210/mend-5-12-2005 · Cited by 258
Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus
Lang, S. M., Weeger, M., Stahl-Hennig, C., Coulibaly, C., Hunsmann, G., Müller, J., Müller-Hermelink, H., Fuchs, D., Wachter, H., & Daniel, M. M. (1993) · Journal of Virology · doi:10.1128/jvi.67.2.902-912.1993 · Cited by 221
Diversity of γδ T-cell receptors on murine intestinal intraepithelial lymphocytes
Takagaki, Y., DeCloux, A., Bonneville, M., & Tonegawa, S. (1989) · Nature · doi:10.1038/339712a0 · Cited by 217
Latent equid herpesviruses 1 and 4: detection and distinction using the polymerase chain reaction and co-cultivation from lymphoid tissues.
Welch, H. M., Bridges, C. G., Lyon, A. M., Griffiths, L., & Edington, N. (1992) · Journal of General Virology · doi:10.1099/0022-1317-73-2-261 · Cited by 203
Interferon consensus sequence-binding protein, a member of the interferon regulatory factor family, suppresses interferon-induced gene transcription.
Nelson, N., Marks, M. S., Driggers, P. H., & Ozato, K. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.1.588 · Cited by 192
Substrate specificity, gene structure and tissue-specific distribution of multiple human 3α-hydroxysteroid dehydrogenases
Khanna, M., Qin, K.-N., Wang, R. W., & Cheng, K.-C. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.34.20162 · Cited by 174
Overexpression of the gag-pol precursor from human immunodeficiency virus type 1 proviral genomes results in efficient proteolytic processing in the absence of virion production
Park, J., & Morrow, C. D. (1991) · Journal of Virology · doi:10.1128/jvi.65.9.5111-5117.1991 · Cited by 163
Levels of circulating cell-free nuclear and mitochondrial DNA in benign and malignant ovarian tumors
Zachariah, R. R., Schmid, S., Buerki, N., Radpour, R., Holzgreve, W., & Zhong, X. (2008) · Obstetrics & Gynecology · doi:10.1097/aog.0b013e3181867bc0 · Cited by 142
Genomic organization of the glycoprotein D gene: Duffy blood group Fya/Fyb alloantigen system is associated with a polymorphism at the 44-amino acid residue
Iwamoto, S., Omi, T., Kajii, E., & Ikemoto, S. (1995) · Blood · doi:10.1182/blood.v85.3.622.bloodjournal853622 · Cited by 126
Detection of Mycobacterium bovis in tissues by polymerase chain reaction
Wards, B. (1995) · Veterinary Microbiology · doi:10.1016/0378-1135(94)00096-f · Cited by 114
Cryptic species conservation: a review
Hending, D. (2025) · Biological Reviews · doi:10.1111/brv.13139 · Cited by 113
Induction of the peroxisome proliferator activated receptor by fenofibrate in rat liver
Gebel, T., Arand, M., & Oesch, F. (1992) · FEBS Letters · doi:10.1016/0014-5793(92)80734-x · Cited by 106
Abortion of virologically negative foetuses following experimental challenge of pregnant pony mares with equid herpesvirus 1.
SMITH, K. C., WHITWELL, K. E., BINNS, M. M., DOLBY, C. A., HANNANT, D., & MUMFORD, J. A. (1992) · Equine Veterinary Journal · doi:10.1111/j.2042-3306.1992.tb02830.x · Cited by 102
Morphometric assessment of the dermal microcirculation in patients with chronic venous insufficiency
Pappas, P. J., DeFouw, D. O., Venezio, L. M., Gorti, R., Padberg, F. T., Silva, M. B., Goldberg, M. C., Durán, W. N., & Hobson, R. W. (1997) · Journal of Vascular Surgery · doi:10.1016/s0741-5214(97)70091-0 · Cited by 97
HLA DQA, DQB, and DRB genotyping by oligonucleotide analysis: distribution of alleles and haplotypes in British caucasoids
Doherty, D. G., Vaughan, R. W., Donaldson, P. T., & Mowat, A. P. (1992) · Human Immunology · doi:10.1016/0198-8859(92)90085-2 · Cited by 100
Human Endogenous Retroviral Element K10 (HERV-K10) Encodes a Full-Length Gag Homologous 73-kDa Protein and a Functional Protease
Mueller-Lantzsch, N., Sauter, M., Weiskircher, A., Kramer, K., Best, B., Buck, M., & Grässer, F. (1993) · AIDS Research and Human Retroviruses · doi:10.1089/aid.1993.9.343 · Cited by 98
Autoantibodies against integral membrane proteins of the nuclear envelope in patients with primary biliary cirrhosis
Nickowitz, R. E., Wozniak, R. W., Schaffner, F., & Worman, H. J. (1994) · Gastroenterology · doi:10.1016/s0016-5085(94)95333-3 · Cited by 96
Analysis of the gene sequences of the insulin receptor and the insulin-sensitive glucose transporter (GLUT-4) in patients with common-type non-insulin-dependent diabetes mellitus.
Kusari, J., Verma, U. S., Buse, J. B., Henry, R. R., & Olefsky, J. M. (1991) · Journal of Clinical Investigation · doi:10.1172/jci115437 · Cited by 90
Expression, purification, and characterization of the Drosophila kinesin motor domain produced in Escherichia coli.
Gilbert, S. P., & Johnson, K. A. (1993) · Biochemistry · doi:10.1021/bi00068a028 · Cited by 88
Sequence and structural analysis of surface protein antigen I/II (SpaA) of Streptococcus sobrinus
LaPolla, R. J., Haron, J. A., Kelly, C. G., Taylor, W. R., Bohart, C., Hendricks, M., Pyati, J. P., Graff, R. T., Ma, J. K., & Lehner, T. (1991) · Infection and Immunity · doi:10.1128/iai.59.8.2677-2685.1991 · Cited by 84
Canadian Mennonites and individuals residing in the Friesland region of the Netherlands share the same molecular basis of 17?-hydroxylase deficiency
Imai, T., Yanase, T., Waterman, M., Simpson, E., & Pratt, J. (1992) · Human Genetics · doi:10.1007/bf00207050 · Cited by 83
Metallothionein gene expression and metal regulation during preimplantation mouse embryo development (MT mRNA during early development)
Andrews, G. K., Huet-Hudson, Y. M., Paria, B. C., McMaster, M. T., De, S. K., & Dey, S. K. (1991) · Developmental Biology · doi:10.1016/0012-1606(91)90209-l · Cited by 78
Improved read/write cost tradeoff in DNA-based data storage using LDPC codes
Chandak, S., Tatwawadi, K., Lau, B., Mardia, J., Kubit, M., Neu, J., Griffin, P., Wootters, M., Weissman, T., & Ji, H. (2019) · 2019 57th Annual Allerton Conference on Communication, Control, and Computing (Allerton) · doi:10.1109/allerton.2019.8919890 · Cited by 80
A frequent tyrosinase gene mutation in classic, tyrosinase-negative (type IA) oculocutaneous albinism
Giebel, L. B., Strunk, K. M., King, R. A., Hanifin, J. M., & Spritz, R. A. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.9.3255 · Cited by 74
Malaria in pregnancy in rural Mozambique: the role of parity, submicroscopic and multiple Plasmodium falciparum infections
Saute, F., Menendez, C., Mayor, A., Aponte, J., Gomez‐Olive, X., Dgedge, M., & Alonso, P. (2002) · Tropical Medicine & International Health · doi:10.1046/j.1365-3156.2002.00831.x · Cited by 74
Normal Insulin-Receptor cDNA Sequence in Pima Indians With NIDDM
Moller, D. E., Yokota, A., & Flier, J. S. (1989) · Diabetes · doi:10.2337/diab.38.11.1496 · Cited by 76
The - and -subunits are required for expression of catalytic activity in the hetero-dimeric glucosidase II complex from human liver
Treml, K., Meimaroglou, D., Hentges, A., & Bause, E. (2000) · Glycobiology · doi:10.1093/glycob/10.5.493 · Cited by 63
Tidal and diel orchestration of behaviour and gene expression in an intertidal mollusc
Schnytzer, Y., Simon-Blecher, N., Li, J., Waldman Ben-Asher, H., Salmon-Divon, M., Achituv, Y., Hughes, M. E., & Levy, O. (2018) · Scientific Reports · doi:10.1038/s41598-018-23167-y · Cited by 58
Polymerase chain reaction and synthetic DNA probes: a means of distinguishing the causative agents of syphilis and yaws?
Noordhoek, G. T., Wieles, B., van der Sluis, J. J., & van Embden, J. D. (1990) · Infection and Immunity · doi:10.1128/iai.58.6.2011-2013.1990 · Cited by 60
Growth factor-dependent inhibition of normal hematopoiesis by N-ras antisense oligodeoxynucleotides.
Skorski, T., Szczylik, C., Ratajczak, M. Z., Malaguarnera, L., Gewirtz, A. M., & Calabretta, B. (1992) · The Journal of experimental medicine · doi:10.1084/jem.175.3.743 · Cited by 52
Random variation and concentration effects in PCR
Jagers, P., & Klebaner, F. (2003) · Journal of Theoretical Biology · doi:10.1016/s0022-5193(03)00166-8 · Cited by 49
Gene amplification for c-erbB-2, c-myc, epidermal growth factor receptor, int-2, and N-myc measured by quantitative PCR with a multiple competitor template
Sestini, R., Orlando, C., Zentilin, L., Lami, D., Gelmini, S., Pinzani, P., Giacca, M., & Pazzagli, M. (1995) · Clinical Chemistry · doi:10.1093/clinchem/41.6.826 · Cited by 44
Modelling the PCR amplification process by a size-dependent branching process and estimation of the efficiency
Lalam, N., Jacob, C., & Jagers, P. (2004) · Advances in Applied Probability · doi:10.1239/aap/1086957587 · Cited by 46
A new mobile genetic element inLactobacillus delbrueckii subsp.bulgaricus
Germond, J.-E., Lapierre, L., Delley, M., & Molle, B. (1995) · Molecular and General Genetics MGG · doi:10.1007/bf02191640 · Cited by 33
The elastin gene is disrupted in a family with a balanced translocation t(7;16)(q11.23;q13) associated with a variable expression of the Williams-Beuren syndrome
Duba, H.-C., Doll, A., Neyer, M., Erdel, M., Mann, C., Hammerer, I., Utermann, G., & Grzeschik, K.-H. (2002) · European Journal of Human Genetics · doi:10.1038/sj.ejhg.5200812 · Cited by 31
Interferon Consensus sequence-Binding Protein, a Member of the Interferon Regulatory Factor Family, Suppresses Interferon-Induced Gene Transcription
Nelson, N., Marks, M. S., Driggers, P. H., & Ozato, K. (1993) · Molecular and Cellular Biology · doi:10.1128/mcb.13.1.588-599.1993 · Cited by 192
Confidence intervals for nonhomogeneous branching processes and polymerase chain reactions
Piau, D. (2005) · The Annals of Probability · doi:10.1214/009117904000000775 · Cited by 14
“Weiss and von Haeseler (1997) applied their coalescent method to the data set of Saiki et al. (1988). The efficiency sequence (λk) is not provided; neither is the initial population S0. However, following Weiss and von Haeseler (1997), the extent of the amplification after 20, 25 and 30 cycles [provided by Saiki et al…”
BackgroundMethodInfluential
Determining Platypus Relationships
Gemmell, N., Grant, T., Western, P., Walmsley, J., Watson, J., Murray, N., & Graves, J. (1995) · Australian Journal of Zoology · doi:10.1071/zo9950283 · Cited by 10
Dietary DNA Metabarcoding From Animal Fecal Samples
McConnell, R. D., Jarrett, C., Ferreira, D. F., Powell, L. L., Quiñones, A. L. S., Dominoni, D. M., & Welch, A. J. (2026) · Current Protocols · doi:10.1002/cpz1.70226 · Cited by 2
“The development of PCR (Saiki et al., 1988) and Sanger sequencing (Sanger et al., 1977) enabled the amplification and detection of minuscule quantities of prey DNA from degraded samples (Höss et al., 1992; King et al., 2008).”

Authors: Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A.
Affiliations: Cetus Corporation, Department of Human Genetics, Emeryville, CA 94608.
Cetus Corporation, Department of Microbial Genetics, Emeryville, CA 94608.
Venue: Science
Publisher: American Association for the Advancement of Science (AAAS)
Published: 1988-01-29
Volume: 239
Issue: 4839
Pages: 487-491
ISSN: 0036-8075 (ISSN-L)
1095-9203
DOI: 10.1126/science.2448875
OpenAlex: W2032118018
Cited by: 17,719
References: 19
Citations by year: 2026: 85 · 2025: 101 · 2024: 109 · 2023: 181 · 2022: 113 · 2021: 150 · 2020: 161 · 2019: 152 · 2018: 161 · 2017: 165 · 2016: 210 · 2015: 199 · 2014: 258 · 2013: 238 · 2012: 236
FWCI: 504.53
Type: journal article
Language: en
Open access: closed
Locations: Science (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA and Nucleic Acid Chemistry
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords: PCR, thermostable DNA polymerase, Thermus aquaticus, DNA amplification
MeSH terms: Cloning, Molecular, DNA, DNA-Directed DNA Polymerase, DNA, Recombinant, Electrophoresis, Agar Gel, Globins, Hot Temperature, Humans, Nucleic Acid Denaturation, Nucleic Acid Hybridization, RNA, Thermus, Nucleic Acid Amplification Techniques

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Citations

Cites

Cited by

Related

Metadata

Record

About these checks