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Cited by 30Multiple Sclerosis Research StudiesNeurogenesis and neuroplasticity mechanismsMesenchymal stem cell research

Myelin repair and functional recovery mediated by neural cell transplantation in a mouse model of multiple sclerosis

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Bai, Lianhua, Hecker, Jordan, Kerstetter, Amber, Miller, Robert H.·2013·Neuroscience Bulletin
DOI 10.1007/s12264-013-1312-4PMC PMC4117317
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Abstract

Cellular therapies are becoming a major focus for the treatment of demyelinating diseases such as multiple sclerosis (MS), therefore it is important to identify the most effective cell types that promote myelin repair. Several components contribute to the relative benefits of specific cell types including the overall efficacy of the cell therapy, the reproducibility of treatment, the mechanisms of action of distinct cell types and the ease of isolation and generation of therapeutic populations. A range of distinct cell populations promote functional recovery in animal models of MS including neural stem cells and mesenchymal stem cells derived from different tissues. Each of these cell populations has advantages and disadvantages and likely works through distinct mechanisms. The relevance of such mechanisms to myelin repair in the adult central nervous system is unclear since the therapeutic cells are generally derived from developing animals. Here we describe the isolation and characterization of a population of neural cells from the adult spinal cord that are characterized by the expression of the cell surface glycoprotein NG2. In functional studies, injection of adult NG2(+) cells into mice with ongoing MOG35-55-induced experimental autoimmune encephalomyelitis (EAE) enhanced remyelination in the CNS while the number of CD3(+) T cells in areas of spinal cord demyelination was reduced approximately three-fold. In vivo studies indicated that in EAE, NG2(+) cells stimulated endogenous repair while in vitro they responded to signals in areas of induced inflammation by differentiating into oligodendrocytes. These results suggested that adult NG2(+) cells represent a useful cell population for promoting neural repair in a variety of different conditions including demyelinating diseases such as MS.

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Crossref · 10.1007/s12264-013-1312-4

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  • NG2+ MSC-like cells sourced from other biological systems such as liver and central nervous system can generate functional cells for tissue repair.

    “These NG2+ MSC-like cells sourced from other biological systems, such as liver and central nervous system (CNS) are found being able to generate functional cells for tissue repair [20, 21].”

    From New advances of NG2-expressing cell subset in marrow mesenchymal stem cells as novel therapeutic tools for liver fibrosis/cirrhosis (2024) · cites this paper as [21]

    The Introduction and Results (pages unavailable) describe NG2+ cells isolated from adult mouse spinal cord that can generate oligodendrocytes and promote functional recovery and myelin repair in an MS mouse model. The paper does not establish the claim for liver-derived NG2+ cells, and its in vivo repair findings do not show that the transplanted cells themselves generated the repairing cells. (LLM, conf=0.86)

    Experimental — accuracy not yet measured

  • PPWP has been stably used for the isolation of NG2+ cells from multiple adult tissues.

    “which has been stably used for the isolation of NG2+ cells from multiple adult tissues [21, 23]”

    From New advances of NG2-expressing cell subset in marrow mesenchymal stem cells as novel therapeutic tools for liver fibrosis/cirrhosis (2024) · cites this paper as [21]

    The Methods section, “Purification of Mouse Adult Spinal Cord NG2+ Cells Using Percoll Gradient” (page unavailable), describes isolating cells from adult mouse spinal cords. The Results section, “Purification and characterization of Adult Mouse spinal cord-Derived NG2+ cells” (page unavailable), reports that more than 98% of cells were NG2+ by the second passage. Our copy does not identify the procedure as PPWP or establish its stable use across multiple adult tissues. (LLM, conf=0.84)

    Experimental — accuracy not yet measured

  • Most passaged m-NG2/BMMSCs exhibited a diamond-shaped flaky morphology similar to cells isolated from multiple other organs but clearly different from spindle-shaped m-BMMSCs.

    “most passaged (P) cells exhibited a diamond-shaped flaky morphology (Fig. 1Aa; Suppl. Fig. S1Bd, pink cartoon marks assumed NG2+ cells), similar to the cells isolated from multiple other organs [21, 23], but those morphologies were clearly different from those of spindle-shaped m-BMMSCs”

    From New advances of NG2-expressing cell subset in marrow mesenchymal stem cells as novel therapeutic tools for liver fibrosis/cirrhosis (2024) · cites this paper as [21]

    The Results section (page unavailable) says most passaged adult mouse spinal-cord NG2+ cells assumed a rhomboid morphology, consistent with the described diamond shape. The cited paper does not establish that this morphology resembles cells isolated from multiple other organs, nor does it compare these cells with spindle-shaped mouse bone-marrow MSCs. (LLM, conf=0.79)

    Experimental — accuracy not yet measured

  • NG2+ cells promote functional recovery in disease models.

    “and promote functional recovery in disease models [21]”

    From New advances of NG2-expressing cell subset in marrow mesenchymal stem cells as novel therapeutic tools for liver fibrosis/cirrhosis (2024) · cites this paper as [21]

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Cited by

Sorted by citations

Authors
Bai, L., Hecker, J., Kerstetter, A., & Miller, R. H.
Affiliations
Case Western Reserve University (US)
Corresponding author
  • Bai, Lianhua
  • Miller, Robert H.
ORCID
Venue
Neuroscience Bulletin
Publisher
Springer Science and Business Media LLC
Published
2013-03-08
Volume
29
Issue
2
Pages
239-250
ISSN
  • 1995-8218 (ISSN-L)
  • 1673-7067
DOI
10.1007/s12264-013-1312-4
PMC
PMC4117317
OpenAlex
W2071145324
Cited by
30
References
1
Citations by year
2024: 5 · 2023: 1 · 2022: 1 · 2021: 1 · 2020: 2 · 2018: 4 · 2017: 2 · 2016: 4 · 2015: 1 · 2014: 6 · 2013: 2 · 2012: 1
FWCI
3.25
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • Neuroscience Bulletin (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health
  • National Institute of Neurological Disorders and Stroke (NS 030800, R01NS030800, NS077942, R01NS077942)
Topics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
  • Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
Keywords
multiple sclerosis, remyelination, neural transplantation, oligodendrocyte precursor cells, adult spinal cord, experimental autoimmune encephalomyelitis, MOG35-55, oligodendrocyte differentiation, demyelination, T cell depletion, cell therapy, demyelinating disease
MeSH terms
Chondroitin Sulfate Proteoglycan 4, Animals, Animals, Newborn, Antigens, Bromodeoxyuridine, Cells, Cultured, Disease Models, Animal, Female, Glial Fibrillary Acidic Protein, Male, Mice, Inbred C57BL, Mice, Transgenic, Multiple Sclerosis, Neurons, Oligodendroglia, Organ Culture Techniques, Peptide Fragments, Proteoglycans, Spinal Cord, Time Factors, Culture Media, Conditioned, CD3 Complex, Cell Transplantation, Myelin Proteolipid Protein, Recovery of Function, Proto-Oncogene Proteins c-sis, Green Fluorescent Proteins, Mice, Myelin-Oligodendrocyte Glycoprotein

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