Repair of liver mediated by adult mouse liver neuro-glia antigen 2-positive progenitor cell transplantation in a mouse model of cirrhosis
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NG2-expressing cells are a population of periportal vascular stem/progenitors (MLpvNG2 + cells) that were isolated from healthy adult mouse liver by using a “Percoll-Plate-Wait” procedure. We demonstrated that isolated cells are able to restore liver function after transplantation into a cirrhotic liver and co-localized with the pericyte marker (immunohistochemistry: PDGFR-β) and CK19. Cells were positive for: stem cell (Sca-1, CD133, Dlk) and liver stem cell markers (EpCAM, CD14, CD24, CD49f); and negative for: hematopoietic (CD34, CD45) and endothelial markers (CD31, vWf, von Willebrand factor). Cells were transplanted (1 × 10 6 cells) in mice with diethylnitrosamine-induced cirrhosis at week 6. Cells showed increased hepatic associated gene expression of alpha-fetoprotein (AFP), Albumin (Alb), Glucose-6-phosphatase (G6Pc), SRY (sex determining region Y)-box 9 (Sox9), hepatic nuclear factors (HNF1a, HNF1β, HNF3β, HNF4α, HNF6, Epithelial cell adhesion molecule (EpCAM), Leucine-rich repeated-containing G-protein coupled receptor 5-positive (Lgr5) and Tyrosine aminotransferase (TAT). Cells showed decreased fibrogenesis, hepatic stellate cell infiltration, Kupffer cells and inflammatory cytokines. Liver function markers improved. In a cirrhotic liver environment, cells could differentiate into hepatic lineages. In addition, grafted MLpvNG2 + cells could mobilize endogenous stem/progenitors to participate in liver repair. These results suggest that MLpvNG2 + cells may be novel adult liver progenitors that participate in liver regeneration.
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PPWP has been stably used for the isolation of NG2+ cells from multiple adult tissues.
“which has been stably used for the isolation of NG2+ cells from multiple adult tissues [21, 23]”
From New advances of NG2-expressing cell subset in marrow mesenchymal stem cells as novel therapeutic tools for liver fibrosis/cirrhosis (2024) · cites this paper as [23]
The Methods section (page not supplied) describes using the Percoll-Plate-Wait procedure to isolate NG2+ cells from adult mouse liver. The Results report that the isolated cells retained similar characteristics through eight passages. Our copy does not establish that the procedure has been used to isolate NG2+ cells from multiple adult tissues. (LLM, conf=0.87)
Experimental — accuracy not yet measuredMost passaged m-NG2/BMMSCs exhibited a diamond-shaped flaky morphology similar to cells isolated from multiple other organs but clearly different from spindle-shaped m-BMMSCs.
“most passaged (P) cells exhibited a diamond-shaped flaky morphology (Fig. 1Aa; Suppl. Fig. S1Bd, pink cartoon marks assumed NG2+ cells), similar to the cells isolated from multiple other organs [21, 23], but those morphologies were clearly different from those of spindle-shaped m-BMMSCs”
From New advances of NG2-expressing cell subset in marrow mesenchymal stem cells as novel therapeutic tools for liver fibrosis/cirrhosis (2024) · cites this paper as [23]
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Cites
Clonogenic assay of cells in vitro
Franken, N. A. P., Rodermond, H. M., Stap, J., HAVEMAN, J., & Chris van, B. R. E. E. (2006) · Nature Protocols · doi:10.1038/nprot.2006.339 · Cited by 4,202 · see citing works
A perivascular origin for mesenchymal stem cells in multiple human organs
Crisan, M., Yap, S., Casteilla, L., Chen, C.-W., Corselli, M., Park, T. S., Andriolo, G., Sun, B., Zheng, B., Zhang, L., et al. (2008) · Cell Stem Cell · doi:10.1016/j.stem.2008.07.003 · Cited by 3,991 · see citing works
Organoid Models and Applications in Biomedical Research
Xinaris, C., Brizi, V., & Remuzzi, G. (2015) · The Nephron journals/Nephron journals · doi:10.1159/000433566 · Cited by 3,517 · see citing works
The blood–brain barrier: an overview
Ballabh, P., Braun, A., & Nedergaard, M. (2004) · Neurobiology of Disease · doi:10.1016/j.nbd.2003.12.016 · Cited by 2,276 · see citing works
Prevention and management of gastroesophageal varices and variceal hemorrhage in cirrhosis
García–Tsao, G., Sanyal, A. J., Grace, N. D., & Carey, W. (2007) · Hepatology · doi:10.1002/hep.21907 · Cited by 1,714 · see citing works
In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration
Huch, M., Dorrell, C., Boj, S. F., van Es, J. H., Li, V. S. W., van de Wetering, M., Sato, T., Hamer, K., Sasaki, N., Finegold, M. J., et al. (2013) · Nature · doi:10.1038/nature11826 · Cited by 1,555 · see citing works
Pericytes in the microvasculature
Hirschi, K. K., & D'Amore, P. A. (1996) · Cardiovascular Research · doi:10.1016/s0008-6363(96)00063-6 · Cited by 763 · see citing works
Caplan, A. I. (2008) · Cell stem cell · doi:10.1016/j.stem.2008.08.008 · Cited by 695 · see citing works
Sheṕro, D., & Morel-Laurens, N. (1993) · The FASEB Journal · doi:10.1096/fasebj.7.11.8370472 · Cited by 652 · see citing works
Preparing the ground for tissue regeneration: from mechanism to therapy
Forbes, S. J., & Rosenthal, N. A. (2014) · Nature Medicine · doi:10.1038/nm.3653 · Cited by 585 · see citing works
Sims, D. (1986) · Tissue and Cell · doi:10.1016/0040-8166(86)90026-1 · Cited by 582 · see citing works
Stem/progenitor cells in liver development, homeostasis, regeneration, and reprogramming
Miyajima, A., Tanaka, M., & Itoh, T. (2014) · Cell Stem Cell · doi:10.1016/j.stem.2014.04.010 · Cited by 570 · see citing works
Bai, L., Lennon, D. P., Eaton, V. L., Maier, K., Caplan, A. I., Miller, S. D., & Miller, R. H. (2009) · Glia · doi:10.1002/glia.20841 · Cited by 539 · see citing works
Self-citationBipotential Adult Liver Progenitors Are Derived from Chronically Injured Mature Hepatocytes
Tarlow, B. D., Pelz, C., Naugler, W. E., Wakefield, L., Wilson, E. M., Finegold, M. J., & Grompe, M. (2014) · Cell Stem Cell · doi:10.1016/j.stem.2014.09.008 · Cited by 533 · see citing works
Terai, S., Ishikawa, T., Ômori, K., Aoyama, K., Marumoto, Y., Urata, Y., Yokoyama, Y., Uchida, K., Yamasaki, T., Fujii, Y., et al. (2006) · Stem Cells · doi:10.1634/stemcells.2005-0542 · Cited by 491 · see citing works
Hepatic progenitor cells of biliary origin with liver repopulation capacity
Lu, W.-Y., Bird, T. G., Boulter, L., Tsuchiya, A., Cole, A. M., Hay, T., Guest, R. V., Wojtacha, D., Man, T. Y., Mackinnon, A., et al. (2015) · Nature Cell Biology · doi:10.1038/ncb3203 · Cited by 479 · see citing works
Hepatocyte growth factor twenty years on: Much more than a growth factor
Nakamura, T., Sakai, K., Nakamura, T., & Matsumoto, K. (2011) · Journal of Gastroenterology and Hepatology · doi:10.1111/j.1440-1746.2010.06549.x · Cited by 472 · see citing works
Fate tracing of mature hepatocytes in mouse liver homeostasis and regeneration
Malato, Y., Naqvi, S., Schürmann, N., Ng, R., Wang, B., Zape, J., Kay, M. A., Grimm, D., & Willenbring, H. (2011) · Journal of Clinical Investigation · doi:10.1172/jci59261 · Cited by 434 · see citing works
Adult Hepatocytes Are Generated by Self-Duplication Rather than Stem Cell Differentiation
Yanger, K., Knigin, D., Zong, Y., Maggs, L., Gu, G., Akiyama, H., Pikarsky, E., & Stanger, B. Z. (2014) · Cell Stem Cell · doi:10.1016/j.stem.2014.06.003 · Cited by 426 · see citing works
Isolation and characterisation of mesenchymal stem cells from adult mouse bone marrow
Tropel, P., Noël, D., Platet, N., Legrand, P., Benabid, A., & Berger, F. R. (2004) · Experimental Cell Research · doi:10.1016/j.yexcr.2003.12.030 · Cited by 419 · see citing works
CNS Microvascular Pericytes Exhibit Multipotential Stem Cell Activity
Dore-Duffy, P., Katychev, A., Wang, X., & Van Buren, E. (2006) · Journal of Cerebral Blood Flow & Metabolism · doi:10.1038/sj.jcbfm.9600272 · Cited by 414 · see citing works
Rustad, K. C., Wong, V., Sorkin, M., Glotzbach, J. P., Major, M. R., Rajadas, J., Longaker, M. T., & Gurtner, G. C. (2011) · Biomaterials · doi:10.1016/j.biomaterials.2011.09.041 · Cited by 401 · see citing works
Hepatic oval cells express the hematopoietic stem cell marker thy-1 in the rat
Petersen, B. E., Goff, J. P., Greenberger, J. S., & Michalopoulos, G. K. (1998) · Hepatology · doi:10.1002/hep.510270218 · Cited by 358 · see citing works
Liver Progenitor Cells Yield Functional Hepatocytes in Response to Chronic Liver Injury in Mice
Español–Suñer, R., Carpentier, R., Van Hul, N., Legry, V., Achouri, Y., Cordi, S., Jacquemin, P., Lemaigre, F., & Leclercq, I. A. (2012) · Gastroenterology · doi:10.1053/j.gastro.2012.08.024 · Cited by 356 · see citing works
Klein, I., Cornejo, J. C., Polakos, N. K., John, B., Wuensch, S. A., Topham, D. J., Pierce, R. H., & Crispe, I. N. (2007) · Blood · doi:10.1182/blood-2007-02-073841 · Cited by 326 · see citing works
Mechanical Strength and Biocompatibility of Ultrafine-Grained Commercial Purity Titanium
Estrin, Y., Kim, H., Lapovok, R., Ng, H. P., & Jo, J.-H. (2013) · BioMed Research International · doi:10.1155/2013/914764 · Cited by 313 · see citing works
Role of the CNS microvascular pericyte in the blood-brain barrier
Balabanov, R. D., & Dore‐Duffy, P. (1998) · Journal of Neuroscience Research · doi:10.1002/(sici)1097-4547(19980915)53:6 · Cited by 304 · see citing works
Perivascular cells for regenerative medicine
Crisan, M., Corselli, M., Chen, W. C. W., & Péault, B. (2012) · Journal of Cellular and Molecular Medicine · doi:10.1111/j.1582-4934.2012.01617.x · Cited by 299 · see citing works
Schaub, J. R., Malato, Y., Gormond, C., & Willenbring, H. (2014) · Cell Reports · doi:10.1016/j.celrep.2014.07.003 · Cited by 254 · see citing works
Liver diseases and aging: friends or foes?
Sheedfar, F., Biase, S. D., Koonen, D. P. Y., & Vinciguerra, M. (2013) · Aging Cell · doi:10.1111/acel.12128 · Cited by 247 · see citing works
Identification of adult hepatic progenitor cells capable of repopulating injured rat liver
Yovchev, M. I., Grozdanov, P. N., Zhou, H., Racherla, H., Guha, C., & Dabeva, M. D. (2007) · Hepatology · doi:10.1002/hep.22047 · Cited by 241 · see citing works
Mouse A6-Positive Hepatic Oval Cells Also Express Several Hematopoietic Stem Cell Markers
Petersen, B. E., Grossbard, B. L., Hatch, H. M., Pi, L., Deng, J., & Scott, E. W. (2003) · Hepatology · doi:10.1053/jhep.2003.50104 · Cited by 237 · see citing works
Prospective isolation of a bipotential clonogenic liver progenitor cell in adult micePreprint
Dorrell, C., Erker, L. R., Schug, J., Kopp, J. L., Canaday, P. S., Fox, A. J., Smirnova, O., Duncan, A. W., Finegold, M. J., Sander, M., et al. (2011) · Genes & Development · doi:10.1101/gad.2029411 · Cited by 236 · see citing works
Carpino, G., Cardinale, V., Onori, P., Franchitto, A., Berloco, P. B., Rossi, M., Wang, Y., Semeraro, R., Anceschi, M. M., Brunelli, R., et al. (2011) · Journal of Anatomy · doi:10.1111/j.1469-7580.2011.01462.x · Cited by 224 · see citing works
Volarević, V., Nurković, J., Arsenijević, N., & Stojković, M. (2014) · Stem Cells · doi:10.1002/stem.1818 · Cited by 213 · see citing works
Oertel, M., Menthena, A., Dabeva, M. D., & Shafritz, D. A. (2006) · Gastroenterology · doi:10.1053/j.gastro.2005.10.049 · Cited by 210 · see citing works
Critical Review of Clinical Trials of Bone Marrow Stem Cells in Liver Disease
Houlihan, D. D., & Newsome, P. N. (2008) · Gastroenterology · doi:10.1053/j.gastro.2008.05.040 · Cited by 189 · see citing works
The biliary epithelium gives rise to liver progenitor cells
Rodrigo-Torres, D., Affò, S., Coll, M., Morales-Ibanez, O., Millán, C., Blaya, D., Alvarez-Guaita, A., Rentero, C., José Lozano, J., Maestro, M. A., et al. (2014) · Hepatology · doi:10.1002/hep.27078 · Cited by 189 · see citing works
Evidence for label-retaining tumour-initiating cells in human glioblastoma
Deleyrolle, L. P., Harding, A., Cato, K., Siebzehnrubl, F. A., Rahman, M., Azari, H., Olson, S., Gabrielli, B., Osborne, G., Vescovi, A., et al. (2011) · Brain · doi:10.1093/brain/awr081 · Cited by 182 · see citing works
Mohamadnejad, M., Alimoghaddam, K., Bagheri, M., Ashrafi, M., Abdollahzadeh, L., Akhlaghpoor, S., Bashtar, M., Ghavamzadeh, A., & Malekzadeh, R. (2013) · Liver International · doi:10.1111/liv.12228 · Cited by 182 · see citing works
Busch, S. A., Horn, K. P., Cuascut, F. X., Hawthorne, A. L., Bai, L., Miller, R. H., & Silver, J. (2010) · Journal of Neuroscience · doi:10.1523/jneurosci.3705-09.2010 · Cited by 179 · see citing works
Self-citationStallcup, W. B. (1981) · Developmental Biology · doi:10.1016/s0012-1606(81)80018-8 · Cited by 150 · see citing works
Paik, Y. H., Kim, J. K., Lee, J. I., Kang, S. H., Kim, D. Y., An, S. H., Lee, S. J., Lee, D. K., Han, K.-H., Chon, C. Y., et al. (2009) · Gut · doi:10.1136/gut.2008.157420 · Cited by 126 · see citing works
Mesenchymal stem cells and Interleukin-6 attenuate liver fibrosis in mice
Nasir, G. A., Mohsin, S., Khan, M., Shams, S., Ali, G., Khan, S. N., & Riazuddin, S. A. (2013) · Journal of Translational Medicine · doi:10.1186/1479-5876-11-78 · Cited by 125 · see citing works
Adaptive remodeling of the biliary architecture underlies liver homeostasis
Kaneko, K., Kamimoto, K., Miyajima, A., & Itoh, T. (2015) · Hepatology · doi:10.1002/hep.27685 · Cited by 108 · see citing works
MacFadyen, J. R., Savage, K., Wienke, D., & Isacke, C. M. (2006) · Gene Expression Patterns · doi:10.1016/j.modgep.2006.07.006 · Cited by 97 · see citing works
Iwasa, J., Shimizu, M., Shiraki, M., Shirakami, Y., Sakai, H., Terakura, Y., Takai, K., Tsurumi, H., Tanaka, T., & Moriwaki, H. (2009) · Cancer Science · doi:10.1111/j.1349-7006.2009.01402.x · Cited by 87 · see citing works
Zeng, X. Y., Lin, Y., Yin, C., Zhang, X., Ning, B., Zhang, Q., Zhang, J., Qiu, L., Qin, X.-R., Chen, Y.-X., et al. (2010) · Hepatology · doi:10.1002/hep.24647 · Cited by 82 · see citing works
CD24-Positive Cells from Normal Adult Mouse Liver Are Hepatocyte Progenitor Cells
Qiu, Q., Hernandez, J. C., Dean, A. M., Rao, P. H., & Darlington, G. J. (2011) · Stem Cells and Development · doi:10.1089/scd.2010.0352 · Cited by 79 · see citing works
Harn, H., Lin, S., Hung, S.-H., Subeq, Y., Li, Y.-S., Syu, W., Ding, D., Lee, R.-P., Hsieh, D.-K., Lin, P., et al. (2012) · Cell Transplantation · doi:10.3727/096368912x652959 · Cited by 77 · see citing works
Roderfeld, M., Räth, T., Voswinckel, R., Dierkes, C., Dietrich, H., Zahner, D., Graf, J., & Roeb, E. (2009) · Hepatology · doi:10.1002/hep.23274 · Cited by 75 · see citing works
Orlidge, A., & D'Amore, P. A. (1986) · Microvascular Research · doi:10.1016/0026-2862(86)90005-1 · Cited by 73 · see citing works
Gridelli, B. G., Vizzini, G. B., Pietrosi, G., Luca, A., Spada, M., Gruttadauria, S., Cintorino, D., Amico, G., Chinnici, C. M., Miki, T., et al. (2011) · Liver Transplantation · doi:10.1002/lt.22322 · Cited by 72 · see citing works
Cellular Therapy for Liver Disease
Huebert, R. C., & Rakela, J. L. (2014) · Mayo Clinic Proceedings · doi:10.1016/j.mayocp.2013.10.023 · Cited by 71 · see citing works
Lamoury, F. M. J., Croitoru‐Lamoury, J., & Brew, B. J. (2006) · Cytotherapy · doi:10.1080/14653240600735875 · Cited by 67 · see citing works
Saint‐Pol, J., Vandenhaute, E., Boucau, M., Candela, P., Dehouck, L., Cecchelli, R., Dehouck, M., Fénart, L., & Gosselet, F. (2012) · Journal of Alzheimer s Disease · doi:10.3233/jad-2012-112090 · Cited by 66 · see citing works
Rim, J., Mynatt, R. L., & Gawrońska‐Kozak, B. (2005) · The FASEB Journal · doi:10.1096/fj.04-3204fje · Cited by 66 · see citing works
Isolated human islets contain a distinct population of mesenchymal stem cells
Carlotti, F., Zaldumbide, A., Loomans, C. J. M., van Rossenberg, E., Engelse, M. A., de Koning, E. J. P., & Hoeben, R. C. (2010) · Islets · doi:10.4161/isl.2.3.11449 · Cited by 65 · see citing works
Sakthisekaran, D. (2011) · Molecular Medicine Reports · doi:10.3892/mmr.2011.555 · Cited by 61 · see citing works
Russell, K. C., Tucker, H. A., Bunnell, B. A., Andreeff, M., Schober, W., Gaynor, A. S., Strickler, K. L., Lin, S., Lacey, M. R., & O'Connor, K. C. (2013) · Tissue Engineering Part A · doi:10.1089/ten.tea.2012.0649 · Cited by 56 · see citing works
Kim, J., & Ko, J. (2014) · Cell Death and Differentiation · doi:10.1038/cdd.2014.80 · Cited by 56 · see citing works
Perivascular Mesenchymal Progenitors in Human Fetal and Adult Liver
Gerlach, J. C., Over, P., Turner, M. E., Thompson, R. L., Foka, H. G., Chen, W. C. W., Péault, B., Gridelli, B. G., & Schmelzer, E. (2012) · Stem Cells and Development · doi:10.1089/scd.2012.0296 · Cited by 50 · see citing works
Re-evaluation of liver stem/progenitor cells
Tanimizu, N., & Mitaka, T. (2014) · Organogenesis · doi:10.4161/org.27591 · Cited by 46 · see citing works
Liu, B. C., Balkwill, A., Roddam, A., Brown, A., Beral, V., & Collaborators, O. B. O. T. M. W. S. (2008) · American Journal of Epidemiology · doi:10.1093/aje/kwn280 · Cited by 46 · see citing works
Knight, B., Matthews, V. B., Olynyk, J. K., & Yeoh, G. C. (2005) · BioEssays · doi:10.1002/bies.20311 · Cited by 44 · see citing works
Ablation of NG2 Proteoglycan Leads to Deficits in Brown Fat Function and to Adult Onset Obesity
Chang, Y., She, Z., Sakimura, K., Roberts, A. J., Kuchárová, K., Rowitch, D. H., & Stallcup, W. B. (2012) · PLoS ONE · doi:10.1371/journal.pone.0030637 · Cited by 42 · see citing works
PDGFRα in Liver Pathophysiology: Emerging Roles in Development, Regeneration, Fibrosis, and Cancer
Kikuchi, A., & Monga, S. P. (2015) · Gene Expression · doi:10.3727/105221615x14181438356210 · Cited by 42 · see citing works
Chia, L. Y., Walsh, N. C., Martin, T. J., & Sims, N. A. (2015) · Bone · doi:10.1016/j.bone.2014.11.005 · Cited by 42 · see citing works
Human mesenchymal stem cells towards non-alcoholic steatohepatitis in an immunodeficient mouse model
Winkler, S., Borkham‐Kamphorst, E., Stock, P., Brückner, S., Dollinger, M., Weiskirchen, R., & Christ, B. (2014) · Experimental Cell Research · doi:10.1016/j.yexcr.2014.04.017 · Cited by 42 · see citing works
Man, S., Fan, W. Q., Gao, W., Li, Y., Wang, Y., Liu, Z., & Li, H. (2013) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2013.10.051 · Cited by 37 · see citing works
Liver cell therapy and tissue engineering for transplantation
Vacanti, J. P., & Kulig, K. M. (2014) · Seminars in Pediatric Surgery · doi:10.1053/j.sempedsurg.2014.05.001 · Cited by 36 · see citing works
Carbamazepine promotes liver regeneration and survival in mice
Kawaguchi, T., Kodama, T., Hikita, H., Tanaka, S., Shigekawa, M., Nawa, T., Shimizu, S., Li, W., Miyagi, T., Hiramatsu, N., et al. (2013) · Journal of Hepatology · doi:10.1016/j.jhep.2013.07.018 · Cited by 33 · see citing works
Bai, L., Hecker, J., Kerstetter, A., & Miller, R. H. (2013) · Neuroscience Bulletin · doi:10.1007/s12264-013-1312-4 · Cited by 30 · see citing works
Self-citationZhang, Y., Chen, X., & Sun, D. (2014) · The International Journal of Artificial Organs · doi:10.5301/ijao.5000284 · Cited by 29 · see citing works
Self-citationProfessor Toshio Ito: A clairvoyant in pericyte biology.
Suematsu, M., & Aiso, S. (2001) · The Keio Journal of Medicine · doi:10.2302/kjm.50.66 · Cited by 28 · see citing works
Electrospun P34HB fibres: a scaffold for tissue engineering
Fu, N., Deng, S., Fu, Y., Li, G., Cun, X., Hao, L., Wei, X., Cai, X., Peng, Q., & Lin, Y. (2014) · Cell Proliferation · doi:10.1111/cpr.12122 · Cited by 28 · see citing works
Lee, Y., Pan, C., Koeberl, D. D., Mansfield, B. C., & CHOU, J. Y. (2013) · Molecular Genetics and Metabolism · doi:10.1016/j.ymgme.2013.06.014 · Cited by 25 · see citing works
Isolation of EpCAM+/CD133- Hepatic Progenitor Cells
Hao, P., Lee, M.-J., Yu, G., Kim, I., Cho, Y., & Kim, D. (2013) · Molecules and Cells · doi:10.1007/s10059-013-0190-y · Cited by 25 · see citing works
Isolation, Characterization, and Transplantation of Cardiac Endothelial Cells
Pratumvinit, B., Reesukumal, K., Janebodin, K., Ieronimakis, N. M., & Reyes, M. (2013) · BioMed Research International · doi:10.1155/2013/359412 · Cited by 20 · see citing works
Kamisoglu, K., Sukumaran, S., Nouri-Nigjeh, E., Tu, C., Li, J., Shen, X., Duan, X., Qu, J., Almon, R. R., DuBois, D. C., et al. (2015) · OMICS A Journal of Integrative Biology · doi:10.1089/omi.2014.0130 · Cited by 20 · see citing works
Coexpression of CD14 and CD326 Discriminate Hepatic Precursors in the Human Fetal Liver
Fomin, M. E., Tai, L.-K., Bárcena, A., & Muench, M. O. (2010) · Stem Cells and Development · doi:10.1089/scd.2010.0218 · Cited by 18 · see citing works
Pugnale, P., Latorre, P., Rossi, C., Crovatto, K., Pazienza, V., Gottardi, A. D., & Negro, F. (2005) · Journal of Virological Methods · doi:10.1016/j.jviromet.2005.11.007 · Cited by 16 · see citing works
Donor Organ Shortage Crisis: A Case Study Review of a Financial Incentive-Based System
Sebayel, M. A., Alenazi, A., Sabbagh, R., Ageel, T. A., Enazi, M. A., Bahili, H. A., & Elsiesy, H. (2014) · Transplantation Proceedings · doi:10.1016/j.transproceed.2014.06.024 · Cited by 15 · see citing works
Steiger-Luther, N. C. (2010) · Hepatic Medicine Evidence and Research · doi:10.2147/hmer.s7660 · Cited by 9 · see citing works
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Sato, K., Marzioni, M., Meng, F., Francis, H., Glaser, S., & Alpini, G. (2019) · Hepatology · doi:10.1002/hep.30150 · Cited by 423 · see citing works
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“In addition, grafted MLpvNG2(+) cells could mobilize endogenous stem cells to participate in liver regeneration [55].”
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Chen, Q., You, X., Yang, W., Jiang, S., Lai, J., Zhang, H., & Bai, L. (2020) · Life Sciences · doi:10.1016/j.lfs.2019.117121 · Cited by 23 · see citing works
Self-citationZhang, H., Siegel, C., Li, J., Lai, J., Shuai, L., Lai, X., Zhang, Y., Jiang, Y., Bie, P., & Bai, L. (2016) · Journal of Tissue Engineering and Regenerative Medicine · doi:10.1002/term.2311 · Cited by 18 · see citing works
“We previously reported that a population of hepatic progenitor cells (NG2+HPCs) was isolated from adult mouse periportal vascular region using the Percoll– Plate–Wait procedure (Zhang et al., 2016).”
BackgroundMethodInfluentialSelf-citationDecellularized Liver Scaffold for Liver Regeneration
Yang, W., Xia, R., Zhang, Y., Zhang, H., & Bai, L. (2017) · Methods in molecular biology · doi:10.1007/7651_2017_53 · Cited by 18 · see citing works
Self-citationImmunotherapeutic Targeting of NG2/CSPG4 in Solid Organ Cancers
Zhang, H., Wu, Z., Hu, D., Yan, M., Sun, J., Lai, J., & Bai, L. (2022) · Vaccines · doi:10.3390/vaccines10071023 · Cited by 14 · see citing works
“Based on our recent studies, NG2/CSPG4-expressing cells isolated from multiple adult solid organs (the liver, pancreas, heart, lungs, and kidneys) including bone marrow by using the “Percoll−Plate−Wait” procedure method [23] exhibit characteristics of embryonic-like stem cells.”
BackgroundMethodInfluentialSelf-citationLai, J., Jiang, S., Shuai, L., Zhang, Y., Xia, R., Chen, Q., & Bai, L. (2021) · Stem Cell Research · doi:10.1016/j.scr.2021.102477 · Cited by 12 · see citing works
Self-citationSayyed, H., Osama, A., Idriss, N. K., Sabry, D., Abdelrhim, A. S., & Bakry, R. (2016) · Europe PMC (PubMed Central) · Cited by 11 · see citing works
Chen, Q.-Y., Yan, M., Lin, H., Lai, J., Yang, Z., Hu, D.-Y., Deng, Y., Shi, S.-Y., Shuai, L., Zhang, L.-D., et al. (2023) · Cell Death Discovery · doi:10.1038/s41420-023-01313-4 · Cited by 11 · see citing works
“Four time periods are noted in the DEN mouse model [43]: the phase of LH (0 weeks post-DEN), the liver homeostatic phase (LFP, 3–6 weeks post-DEN), the liver cirrhotic phase (LCP, 7–10 weeks post-DEN) and HCC (11–15 weeks post-DEN) [5].”
BackgroundSelf-citationSingle‐cell landscape of the intrahepatic ecosystem in alcohol‐related liver disease
Zhao, X., Wang, S., Liu, Q., Wei, W., Sun, X., Song, H., Xu, J., Zhang, S., Wang, H., & Fu, J. (2025) · Clinical and Translational Medicine · doi:10.1002/ctm2.70198 · Cited by 9 · see citing works
Liao, C., Chiu, C.-J., Yang, Y., & Chiang, B. (2022) · iScience · doi:10.1016/j.isci.2022.104262 · Cited by 9 · see citing works
“…Chaboissier et al., 2004; Chung et al., 2013; Holembowski et al., 2014; Horst et al., 2010; Teo et al., 2011; Wang et al., 2013; Yang et al., 2013; Zhang et al., 2016 STAR+METHODS Detailed methods are provided in the online version of this paper and include the following: d KEY RESOURCES TABLE d…”
MethodShiota, T., Matsuda, M., Zheng, X., Nagata, N., Ishii, K., Suzuki, R., Muramatsu, M., Takimoto, K., Hanaki, K., Lemon, S. M., et al. (2022) · Journal of Virology · doi:10.1128/jvi.01496-22 · Cited by 8 · see citing works
“The following primers were used: 59-TGCGTTCCTGCT GTGCTTCTCCA-39 and 59-TTCTCCGTCATCTCCATAGGGATC-39 (Ifnb), 59-AGCAATGGCCTGGGACCTAAA-39 and 59-AGCCGGCACACCAATCTT-39 (Isg15), 59- CAGAAGCACACATTGA AGAA-39 and 59-TGTAAGTAGCCAGAGGAAGG-39 (Ifit1) (33), 59- GGGAAAGCAGAGGAAATCAA-39 and 59-TG AAAGTTGCCATACAGAAG-39 (Ifit2) (34)…”
Chen, Q., You, Y., Zhang, Y., Zhang, H., & Bai, L. (2020) · International Immunopharmacology · doi:10.1016/j.intimp.2020.107180 · Cited by 7 · see citing works
Self-citationZhang, H., Chen, Q., Hu, D., Lai, J., Yan, M., Wu, Z., Yang, Z., Zheng, S., Liu, W., Zhang, L., et al. (2023) · Experimental Cell Research · doi:10.1016/j.yexcr.2023.113867 · Cited by 7 · see citing works
Self-citationHu, D., Lai, J., Chen, Q., & Bai, L. (2024) · Stem Cell Research & Therapy · doi:10.1186/s13287-024-03817-x · Cited by 6 · see citing works
“…for liver disorders, and mesenchymal dermal tissue-sourced stem cells (MSCs), such as MSCs generated from bone marrow ( BM MSCs) [41], adipose tissue [42], the umbilical cord [43], the liver [23, 44], and multiple organ tissues [45], are the most common adult stem cells used for this purpose.”
BackgroundMethodResultInfluentialSelf-citationAnimal Models and Experimental Research in Medicine
Karapehlivan, M., Gelen, V., & Kükürt, A. (2022) · IntechOpen eBooks · doi:10.5772/intechopen.100993 · Cited by 4 · see citing works
Ye, M., Gao, J., Zheng, Z., Xin, D., Zhang, Z., Zhu, S., Zhang, Q., Zhang, Y., Xu, L., Shen, S., et al. (2026) · International Journal of Extreme Manufacturing · doi:10.1088/2631-7990/ae3347 · Cited by 3 · see citing works
“The mRNA levels of liver function-related genes, such as ALB, AFP , and SOX9 [38,39] , and maturation markers, such as MRP2, HNF1A , and HNF4A [40,41] , were substantially greater in the EPICS-cultured tissues than in the con-trol tissues at 10 6 cells · mL − 1 (Figures 6(h) and (i)).”
Self-citationA novel off-liver sinusoidal progenitor cells in supporting transplantable bioliver engineering
Lai, J., Hu, D., Yan, M., Chen, Q., Shi, H., Yang, Q., Ding, M., Li, L., Zhang, H., & Bai, L. (2025) · Biomaterials Advances · doi:10.1016/j.bioadv.2025.214358 · Cited by 2 · see citing works
Self-citationZhang, H., Lai, J., Jiang, S., Shuai, L., Zhang, Y., Xia, R., Chen, Q., & Bai, L. (2020) · doi:10.21203/rs.3.rs-89980/v1 · Cited by 2 · see citing works
Self-citationAlipio, A. R., Vieira, M. R., Häfeli, T., Hoelting, L., Frey, O., Haj, A. J. E., & Arno, M. C. (2025) · Communications Biology · doi:10.1038/s42003-025-08408-x · Cited by 1 · see citing works
Nestin and Neuron-glial antigen 2 transgenes unveil progenitor units in murine salivary glands
do Valle, I. B., Gomes, N. P., Diniz, I. M. A., de Arruda, J. A. A., Almeida, T. F. A., Santos, M. S., Birbrair, A., von Zeidler, S. V., & Silva, T. A. (2021) · Archives of Oral Biology · doi:10.1016/j.archoralbio.2021.105344 · Cited by 1 · see citing works
Chen, Q., You, X., Lai, J., Jiang, S., Zhang, H., & Bai, L. (2020) · IntechOpen eBooks · doi:10.5772/intechopen.89493
“As the Ng2+HSP has been demonstrated to have a role in tissue repair [70] and failed liver replacement [35] in liver cirrhosis murine model, we recently further demonstrated that the intrahepatic Ng2+HSP cells are capable of more efficiency than extrahepatic BM-MSCs in self-renewal and hepatocyte and cholangiocyte dif…”
BackgroundMethodSelf-citationYan, M., Hu, D., Wu, Z., Lai, J., Zhang, L., Zhang, H., Li, S., & Bai, L. (2022) · IntechOpen eBooks · doi:10.5772/intechopen.106220
Self-citationHu, D., Min, Y., Heng, L., Lai, J., Zhiqing, Y., Shuai, L., Zhe, S., Hongyu, Z., Leida, Z., Li, Z., et al. (2023) · Medical Research Frontiers · doi:10.57237/j.mrf.2023.01.003
“针对限于从异质 BMMSCs群体纯化NG2 + /SP方法, 我们研究团队研发了一种具有自主知识产权的原创技 术(Percoll-Plate-Wait procedure) [25],从培养中的(动 物、人类)BMMSCs 异质群体中成功纯化了 NG2 + /SP”
BackgroundSelf-citationZhang, H., Lin, H., Yang, Z., Hu, D., Yan, M., Zhang, L., & Bai, L. (2023) · Springer eBooks · doi:10.1007/978-981-99-0846-2_58-1
Self-citationZhang, H., Lai, J., Lin, H., Yang, Z., Hu, D., Yan, M., Zhang, L., & Bai, L. (2024) · Springer eBooks · doi:10.1007/978-981-99-7119-0_58
Self-citationHu, D., Lai, J., Chen, Q., & Bai, L. (2024) · Figshare · doi:10.6084/m9.figshare.26197443
Self-citationHu, D., 赖洁娟, Chen, Q., & Bai, L. (2024) · Figshare · doi:10.6084/m9.figshare.26197508
Self-citationHu, D., Lai, J., Chen, Q., & Bai, L. (2024) · Figshare · doi:10.6084/m9.figshare.26745368
Self-citationLive-Cell Imaging of Telocyte-like Cells in Primary Liver Cell Cultures
Bresgen, N., & Kerschbaum, H. (2026) · Cells · doi:10.3390/cells15161476
Yan, M., Hu, D., 赖洁娟, Chen, Q., Yu, J., Ding, M., Li, L., Shi, H., Zhang, H., Chen, Y., et al. (2026) · Stem Cells International · doi:10.1155/sci/7020341
Self-citation
- Authors
- Zhang, H., Siegel, C. T., Shuai, L., Lai, J., Zeng, L., Zhang, Y., Lai, X., Bie, P., & Bai, L.
- Affiliations
- Case Western Reserve University (US)
- Corresponding author
- Bie, Ping
- Bai, Lianhua
- Venue
- Scientific Reports
- Published
- 2016-02-24
- Volume
- 6
- Issue
- 1
- Pages
- 21783-21783
- ISSN
- 2045-2322 (ISSN-L)
- PMC
- PMC4764864
- OpenAlex
- W2418471211
- Cited by
- 30
- References
- 84
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 5 · 2023: 4 · 2022: 5 · 2021: 3 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 1 · 2016: 2
- FWCI
- 1.21
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- Scientific Reports (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- National Natural Science Foundation of China
- Southwest Hospital
- Topics
- Health Sciences › Medicine › Hepatology › Liver physiology and pathology
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Surgery › Tissue Engineering and Regenerative Medicine
- Keywords
- liver cirrhosis, liver regeneration, liver progenitor cells, cell transplantation, liver fibrosis, liver repair
- MeSH terms
- Chondroitin Sulfate Proteoglycan 4, Animals, Antigens, Cell Differentiation, Cell Movement, Cells, Cultured, Kupffer Cells, Liver, Liver Cirrhosis, Liver Regeneration, Male, Mice, Inbred C57BL, Proteoglycans, Stem Cell Transplantation, Hepatic Stellate Cells
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