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Cited by 8Calcium Carbonate Crystallization and InhibitionParathyroid Disorders and TreatmentsZebrafish Biomedical Research Applications

Assessing the ability of zebrafish scales to contribute to the short-term homeostatic regulation of [Ca2+] in the extracellular fluid during calcemic challenges

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Hung, Jacky T., Webb, Sarah E., Palumbo, Carla, Lesniak, Agnieszka M., Shipley, Alan M., Rubinacci, Alessandro, Kunkel, Joseph G., Miller, Andrew L.·2019·Fisheries Science
DOI 10.1007/s12562-019-01353-9
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Abstract

The elasmoid scales of fish represent a significant internal reservoir of calcium ions (Ca²⁺), but little is known about the contribution of these scales to the short-term regulation of Ca²⁺ homeostasis in the extracellular fluid (ECF). This gap in our knowledge is partly due to the technical challenges involved in measuring small Ca²⁺ fluxes around the scales of live fish in real time. Here, we describe a technique for exfoliating, mounting, and culturing intact living zebrafish Danio rerio scales, then subjecting them to examination using an extracellular, non-invasive, surface-scanning ion-selective electrode technique (SIET). In a Ca²⁺-sensitive configuration, the SIET can resolve Ca²⁺ flux values in the low-to-sub picomole/square centimeter/second range, with a spatial resolution of approximately 5 μm. We quantified the Ca²⁺ fluxes into and out of scales under different extracellular calcemic challenges set to mimic a variety of Ca²⁺ concentrations in the ECF and showed that the results were similar to those previously reported from isolated mouse metatarsal bone. Our new data extend our current understanding of the role played by fish scales in the short-term homeostatic regulation of Ca²⁺ concentration in the ECF. They also support the suggestion that scales might provide an inexpensive and complementary model for studying the fundamentals of bone-mediated homeostatic Ca²⁺ regulation and the diseases that result from its dysregulation.

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Authors
Hung, J. T., Webb, S. E., Palumbo, C., Lesniak, A. M., Shipley, A. M., Rubinacci, A., Kunkel, J. G., & Miller, A. L.
Affiliations
  • Hong Kong University of Science and Technology (HK)
  • University of Modena and Reggio Emilia (IT)
  • IRCCS Ospedale San Raffaele (IT)
  • University of New England (US)
Corresponding author
Miller, Andrew L.
ORCID
Venue
Fisheries Science
Publisher
Springer Science and Business Media LLC
Published
2019-09-06
Volume
85
Issue
6
Pages
943-959
ISSN
  • 0919-9268 (ISSN-L)
  • 1444-2906
DOI
10.1007/s12562-019-01353-9
OpenAlex
W2971755105
Cited by
8
References
107
Citations by year
2023: 2 · 2022: 2 · 2021: 2 · 2020: 2
FWCI
0.42
Type
journal article
Language
en
Open access
Licence
Locations
  • Fisheries Science (publishedVersion)
  • IRIS UNIMORE (University of Modena and Reggio Emilia) (submittedVersion, cc-by)
  • Rare & Special e-Zone (The Hong Kong University of Science and Technology) (submittedVersion)
Funders
  • Hong Kong Research Grants Council (16101714, 16100115)
  • Innovation and Technology Commission - Hong Kong (ITCPD/17-9)
Topics
  • Physical Sciences › Materials Science › Biomaterials › Calcium Carbonate Crystallization and Inhibition
  • Health Sciences › Medicine › Nephrology › Parathyroid Disorders and Treatments
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Zebrafish Biomedical Research Applications
Keywords
calcium homeostasis, extracellular calcium, elasmoid scales, calcium flux

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