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Cited by 9Legionella and Acanthamoeba researchAmoebic Infections and TreatmentsOcular Infections and Treatments

The DOI matches its Crossref record; 15 of the 15 references it cites were found in a registry or an index.

Viability and morphological changes of Acanthamoeba spp. cysts after treatment with Effective microorganisms (EM)

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Sampaotong, Tanitta, Lek-Uthai, Usa, Roongruangchai, Jantima, Roongruangchai, Kosol·2016·Journal of Parasitic Diseases
DOI 10.1007/s12639-014-0511-xPMC PMC4927493
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Abstract

Acanthamoeba is a free-living opportunistic protozoan parasite that is found in diverse environments. It can cause keratitis, mostly related to inappropriate use of contact lenses, as well as life threatening diseases including encephalitis, disseminated sinusitis, and skin ulcers. This study investigated morphological changes and fine structures of the cyst form of Acanthamoeba spp. after treatment with effective microorganisms (EM™) using light and scanning electron microscopies. Acanthamoeba cysts treated with 1:2, 1:4, 1:6, and undiluted EM™ showed higher percentages of non-viable cysts than those treated with 1:8, 1:10, 1:100, 1:200, and 1:400 EM™ and at 5 days post-treatment developed from cystic stage to trophozoite stage. Acanthamoeba cysts treated at concentrations of 1:2, 1:4, 1:6, and undiluted EM™ exhibited cytoplasmic clumping and shrinkage of amoeba cells away from cyst walls. The effective EM™ concentration lethal to Acanthamoeba spp. cyst could provide information to monitor the environmental control system.

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Research works printed but not linked: 2

  1. Sangakkara UR (2002) The technology of effective microorganisms-case studies of application. Royal Agricultural College, Cirencester, UK
  2. Szymanski N, Patterson RA (2003) Effective microorganisms (EM) and wastewater systems in future directions for on-site systems: best management practice. In: Patterson RA, Jones MJ (eds) Proceedings of on-site’03 conference. Armidale, NSW, Australia, Lanfax Laboratories, pp 347–354

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Metadata

Authors
Sampaotong, T., Lek-Uthai, U., Roongruangchai, J., & Roongruangchai, K.
Affiliations
  • Mahidol University (TH)
  • Siriraj Hospital (TH)
Venue
Journal of Parasitic Diseases
Publisher
Springer Science and Business Media LLC
Published
2014-08-24
Volume
40
Issue
2
Pages
369-373
ISSN
  • 0971-7196 (ISSN-L)
  • 0975-0703
DOI
10.1007/s12639-014-0511-x
PMC
PMC4927493
OpenAlex
W1978835449
Cited by
9
References
15
Citations by year
2026: 1 · 2023: 2 · 2022: 3 · 2021: 2 · 2019: 1
FWCI
0.00
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
  • Journal of Parasitic Diseases (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • China Medical Board
  • Mahidol University
  • Faculty of Public Health, Mahidol University
  • Faculty of Medicine Siriraj Hospital, Mahidol University
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Legionella and Acanthamoeba research
  • Health Sciences › Medicine › Infectious Diseases › Amoebic Infections and Treatments
  • Health Sciences › Medicine › Ophthalmology › Ocular Infections and Treatments
Keywords
Acanthamoeba, cyst viability, effective microorganisms, keratitis, disinfection, environmental control

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CoChat. (2026). Citation check: Viability and morphological changes of Acanthamoeba spp. cysts after treatment with Effective microorganisms (EM). https://app.cochat.ai/papers/10.1007/s12639-014-0511-x
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Sampaotong, T., Lek-Uthai, U., Roongruangchai, J., & Roongruangchai, K. (2016). Viability and morphological changes of Acanthamoeba spp. cysts after treatment with Effective microorganisms (EM). Journal of Parasitic Diseases. https://doi.org/10.1007/s12639-014-0511-x