The DOI matches its Crossref record; 60 of the 60 references it cites were found in a registry or an index.
A high-fat diet decreases AMPK activity in multiple tissues in the absence of hyperglycemia or systemic inflammation in rats
Open accessAbstract
Consumption of a high-fat diet (HFD) in experimental animal models initiates a series of molecular events and outcomes, including insulin resistance and obesity, that mimic the metabolic syndrome in humans. The relationship among, and order of, the molecular events linking a diet high in fat to pathologies is often unclear. In the present study, we provide several novel insights into the relationship between a HFD and AMP-activated protein kinase (AMPK), a key regulator of cellular metabolism and whole-body energy balance. HFD substantially decreased the activities of both isoforms of AMPK in white adipose tissue, heart, and liver. These decreases in AMPK activity occurred in the absence of decreased AMPK transcription, systemic inflammation, hyperglycemia, or elevated levels of free fatty acids. The HFD-induced decrease in AMPK activity was associated with systemic insulin resistance and hyperleptinemia. In blood, >98 % of AMPK activity was localized in agranulocytes as the α1 isoform. In contrast to the solid tissues studied, AMPK activities were not altered by HFD in granulocytes or agranulocytes. We conclude that HFD-induced obesity causes a broad, non-tissue, or isoform-specific lowering of AMPK activity. Given the central position AMPK plays in whole-body energy balance, this decreased AMPK activity may play a previously unrecognized role in obesity and its associated pathologies.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In the citing paper
“AMPK activity was observed to be suppressed by high-fat diet (HFD) in diverse tissues, including white heart, adipose tissue, and liver [266].”
From AMPK, Mitochondrial Function, and Cardiovascular Disease (2020) · cites this paper as [266]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Xu, H., Barnes, G. T., Yang, Q., Tan, G., Yang, D., Chou, C. J., Sole, J., Nichols, A., Ross, J. S., Tartaglia, L. A., et al. (2003) · Journal of Clinical Investigation · doi:10.1172/jci200319451 · Cited by 6,760
Kahn, B. B., Alquier, T., Carling, D., & Hardie, D. G. (2005) · Cell Metabolism · doi:10.1016/j.cmet.2004.12.003 · Cited by 2,799
Statistical analysis of real-time PCR data
Yuan, J. S., Reed, A., Chen, F., & Stewart, C. N. (2006) · BMC Bioinformatics · doi:10.1186/1471-2105-7-85 · Cited by 2,231
Leptin stimulates fatty-acid oxidation by activating AMP-activated protein kinase
Minokoshi, Y., Kim, Y.-B., Peroni, O. D., Fryer, L. G. D., Müller, C., Carling, D., & Kahn, B. B. (2002) · Nature · doi:10.1038/415339a · Cited by 2,053
Park, E. J., Lee, J. H., Yu, G.-Y., He, G., Ali, S. R., Holzer, R. G., Österreicher, C. H., Takahashi, H., & Karin, M. (2010) · Cell · doi:10.1016/j.cell.2009.12.052 · Cited by 1,764
AMP-activated protein kinase: an energy sensor that regulates all aspects of cell function
Hardie, D. G. (2011) · Genes & Development · doi:10.1101/gad.17420111 · Cited by 1,638
High-fat diet-induced obesity in animal models
Hariri, N., & Thibault, L. (2010) · Nutrition Research Reviews · doi:10.1017/s0954422410000168 · Cited by 1,142
AMP-activated protein kinase signaling in metabolic regulation
Long, Y. C. (2006) · Journal of Clinical Investigation · doi:10.1172/jci29044 · Cited by 1,125
Management of cellular energy by the AMP‐activated protein kinase system
Hardie, D. G., Scott, J. W., Pan, D. A., & Hudson, E. R. (2003) · FEBS Letters · doi:10.1016/s0014-5793(03)00560-x · Cited by 868
Sağ, D., Carling, D., Stout, R. D., & Suttles, J. (2008) · The Journal of Immunology · doi:10.4049/jimmunol.181.12.8633 · Cited by 803
Research works printed but not linked: 1
- Mulligan JD, Gonzalez AA, Kumar R, Davis AJ, Saupe KW (2005) Aging elevates basal AMPK activity and eliminates hypoxic activation of AMPK in mouse liver. J Gerontol A Biol Sci Med Sci 60(1):21–27
Cited by
Sorted by citations
AMPK as a Therapeutic Target for Treating Metabolic Diseases
Day, E. A., Ford, R. J., & Steinberg, G. R. (2017) · Trends in Endocrinology and Metabolism · doi:10.1016/j.tem.2017.05.004 · Cited by 630
Vasamsetti, S. B., Karnewar, S., Kanugula, A. K., Thatipalli, A. R., Kumar, J. M., & Kotamraju, S. (2015) · Diabetes · doi:10.2337/db14-1225 · Cited by 397
Mottillo, E. P., Desjardins, E. M., Crane, J. D., Smith, B. K., Green, A. E., Ducommun, S., Henriksen, T. I., Rebalka, I. A., Razi, A., Sakamoto, K., et al. (2016) · Cell Metabolism · doi:10.1016/j.cmet.2016.06.006 · Cited by 338
Protection of mitochondria prevents high-fat diet–induced glomerulopathy and proximal tubular injury
Szeto, H. H., Liu, S., Soong, Y., Alam, N., Prusky, G. T., & Seshan, S. V. (2016) · Kidney International · doi:10.1016/j.kint.2016.06.013 · Cited by 303
AMPK, Mitochondrial Function, and Cardiovascular Disease
Wu, S., & Zou, M.-H. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21144987 · Cited by 286
Metadata
- Authors
- Lindholm, C. R., Ertel, R. L., Bauwens, J. D., Schmuck, E. G., Mulligan, J. D., & Saupe, K. W.
- Affiliations
- University of Wisconsin–Madison (US)
- Corresponding author
- Mulligan, Jacob D.
- Saupe, Kurt W.
- ORCID
- Schmuck, Eric G.
- Venue
- Journal of Physiology and Biochemistry
- Published
- 2012-09-02
- Volume
- 69
- Issue
- 2
- Pages
- 165-175
- ISSN
- 1138-7548 (ISSN-L)
- 1877-8755
- PMC
- PMC3644018
- OpenAlex
- W1976242359
- Cited by
- 136
- References
- 60
- Citations by year
- 2026: 6 · 2025: 9 · 2024: 6 · 2023: 11 · 2022: 12 · 2021: 9 · 2020: 19 · 2019: 10 · 2018: 14 · 2017: 8 · 2016: 12 · 2015: 5 · 2014: 11 · 2013: 3 · 2012: 1
- FWCI
- 2.57
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Journal of Physiology and Biochemistry (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- Funders
- National Institutes of Health
- National Heart, Lung, and Blood Institute (T32HL007936)
- National Institute of Diabetes and Digestive and Kidney Diseases (R01DK080345)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolism, Diabetes, and Cancer
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adipokines, Inflammation, and Metabolic Diseases
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Diet, Metabolism, and Disease
- Keywords
- high-fat diet, AMPK, AMPK isoforms, obesity, insulin resistance, hyperleptinemia, white adipose tissue, heart, liver, agranulocytes, granulocytes, energy balance, metabolic syndrome, free fatty acids
- MeSH terms
- Animals, Adipose Tissue, Brown, Dietary Fats, Hyperglycemia, Inflammation, Male, Obesity, Organ Specificity, Rats, Sprague-Dawley, Rats, Adipose Tissue, White, AMP-Activated Protein Kinases, Diet, High-Fat
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
