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Cited by 30Biopolymer Synthesis and ApplicationsVitamin K Research StudiesEnzyme Production and Characterization

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Coproduction of menaquinone-7 and nattokinase by Bacillus subtilis using soybean curd residue as a renewable substrate combined with a dissolved oxygen control strategy

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Wang, Han, Sun, Xiaojuan, Wang, Li, Wu, Hefang, Zhao, Genhai, Liu, Hui, Wang, Peng, Zheng, Zhiming·2018·Annals of Microbiology
DOI 10.1007/s13213-018-1372-9
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Abstract

Numerous physiological functions of menaquinone-7 (MK-7) act to reduce vascular calcification, suggesting that MK-7 may be a potential therapy for Alzheimer’s and Parkinson’s disease, and in this study, we attempted to increase the concentration of MK-7 synthesized by Bacillus subtilis natto, a standard nattokinase (NK) producing strain. Different Bacillus subtilis isolates demonstrated positive correlations between MK-7 and NK concentrations. Response surface methodology (RSM) was employed to optimize a culture medium for the simultaneous production of these molecules; the optimized medium contained the following components (%, w/v): soybean curd residue, 12.2; soya peptone, 5.7; lactose, 2.6; and K2HPO4, 0.6. The fermentation process was subsequently optimized based on online feedback control of fermentation process parameters. The dissolved oxygen (DO) concentration played an important role in the production of MK-7 and NK. With increased DO concentrations, the cell growth rate and NK activity increased. In contrast, at low DO concentrations, the concentration of MK-7 rapidly increased during the late fermentation stage. Thus, in this study, the production of MK-7 and NK by Bacillus subtilis was accomplished using soybean curd residue through medium optimization and DO control. This novel coproduction strategy was developed by controlling the aeration rate during the fermentation process. The concentrations of MK-7 and NK achieved in this study reached 91.25 mg/L and 2675.73 U/mL, respectively.

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Metadata

Authors
Wang, H., Sun, X., Wang, L., Wu, H., Zhao, G., Liu, H., Wang, P., & Zheng, Z.
Affiliations
  • University of Science and Technology of China (CN)
  • Chinese Academy of Sciences (CN)
Corresponding author
  • Wang, Peng
  • Zheng, Zhiming
ORCID
Venue
Annals of Microbiology
Publisher
Springer Science and Business Media LLC
Published
2018-09-14
Volume
68
Issue
10
Pages
655-665
ISSN
  • 0003-4649 (ISSN-L)
  • 1590-4261
  • 1869-2044
DOI
10.1007/s13213-018-1372-9
OpenAlex
W2891514938
Cited by
30
References
0
Citations by year
2026: 3 · 2025: 4 · 2024: 3 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 6 · 2019: 5
FWCI
1.25
Type
journal article
Language
en
Open access
Licence
www.springer.com/tdm (tdm)
Locations
Annals of Microbiology (publishedVersion)
Funders
  • The National High Technology Research and Development Program of China (2014AA021704)
  • Major Projects of Science and Technology in Anhui Province (17030801036)
Topics
  • Life Sciences › Immunology and Microbiology › Applied Microbiology and Biotechnology › Biopolymer Synthesis and Applications
  • Health Sciences › Nursing › Nutrition and Dietetics › Vitamin K Research Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
Keywords
menaquinone-7, nattokinase, Bacillus natto, soybean curd residue, co-production, dissolved oxygen control, fermentation optimization, culture medium optimization, air flow rate, renewable substrates, vascular calcification, Alzheimer's disease, Parkinson's disease

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