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Cited by 15Mesenchymal stem cell researchProstate Cancer Treatment and ResearchCancer Cells and Metastasis

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Impact of umbilical cord mesenchymal stromal/stem cell secretome and cord blood serum in prostate cancer progression

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Sousa, André, Coelho, Pedro, Leite, Fernanda, Teixeira, Catarina, Rocha, Ana Catarina, Santos, Inês, Baylina, Pilar, Fernandes, Ruben, Soares, Raquel, Costa, Raquel, Gomes, Andreia·2023·Human Cell
DOI 10.1007/s13577-023-00880-zPMC PMC10110723
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Abstract

Prostate cancer (PCa) is the second most common malignancy in men, and the fifth leading cause of death worldwide. Mesenchymal stromal/stem cells (MSC) have been identified in PCa, although contradictory effects in malignant transformation and tumor progression have been described. Since umbilical cord (UC) MSC and cord blood serum (CBS) are rich in numerous growth and anti-inflammatory factors, UC-MSC secretome and CBS are able to modulate tumor cell proliferation and survival as well as immunity and angiogenesis. In the present study, we address this relationship and investigate the influence of UC-MSC secretome and CBS on two human PCa cell lines (PC3 and LNCaP) and a normal epithelial prostate cell line (HPEpiC). Our results disclosed that upon exposure to UC-MSC-conditioned medium or CBS, both PC3 and LNCaP cells exhibited reduced viability, proliferation, and motility while non-malignant epithelial prostate cells were unaffected. These findings were corroborated by expression analysis of AKT/PI3K signaling pathway, p53 and interleukin genes. UC-MSC and CBS factors decreased the expression of growth-stimulating AKT and PI3K effectors and simultaneously up-regulated the expression of tumor-suppressor p53. Moreover, a more anti-inflammatory expression profile was found in both malignant PCa cell lines. Altogether, these results shed light into possible mechanisms by which UC-MSC and CBS reduce PCa progression, further reinforcing their potential use as novel therapeutic agents in PCa. Graphical abstract

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Fig. 1 Functional assays under UC-MSC secretome. A Cytotoxicity rate in HPEpiC, PC3 and LNCaP cell lines and B proliferation rate in HPEpiC, PC3 and LNCaP cell lines for 0%, 25%, 50%, 75% and 100% UC-MSC secretome concentration. C Motility rate in HPEpiC, PC3 and LNCaP cell lines in the UC-MSC secretome concentration of 100%. D Migratory evaluation of HPEpiC, PC3 and LNCaP cell lines in the UC-MSC secretome concentration of 100%. Symbols above bars represent statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)
Fig. 1 Functional assays under UC-MSC secretome. A Cytotoxicity rate in HPEpiC, PC3 and LNCaP cell lines and B proliferation rate in HPEpiC, PC3 and LNCaP cell lines for 0%, 25%, 50%, 75% and 100% UC-MSC secretome concentration. C Motility rate in HPEpiC, PC3 and LNCaP cell lines in the UC-MSC secretome concentration of 100%. D Migratory evaluation of HPEpiC, PC3 and LNCaP cell lines in the UC-MSC secretome concentration of 100%. Symbols above bars represent statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001)CC BY · PubMed Central
Fig. 2 Functional assays under CBS concentrations. A Cytotoxicity rate in HPEpiC, PC3 and LNCaP cell lines. B Proliferation rate in HPEpiC, PC3 and LNCaP cell lines. C Motility rate in HPEpiC, PC3 and LNCaP cell lines in the CBS concentration of 10%. D Migratory evaluation of HPEpiC, PC3 and LNCaP cell lines in the CBS concentration of 10%. Symbols above bars represent statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001)
Fig. 2 Functional assays under CBS concentrations. A Cytotoxicity rate in HPEpiC, PC3 and LNCaP cell lines. B Proliferation rate in HPEpiC, PC3 and LNCaP cell lines. C Motility rate in HPEpiC, PC3 and LNCaP cell lines in the CBS concentration of 10%. D Migratory evaluation of HPEpiC, PC3 and LNCaP cell lines in the CBS concentration of 10%. Symbols above bars represent statistical significance (*p < 0.05; **p < 0.01; ***p < 0.001)CC BY · PubMed Central
Fig. 3 Gene relative quantification in HPEpiC, PC3 and LNCaP cell lines. A AKT. B PI3K. C p53. D IL-1β. E IL-6. F IL-10. Bars colors in (A)–(E) represents studied groups: groups: Control group; 100% of UC-MSC; 10% of CBS. Symbols above bars represent statistical significance (*p < 0.05; **p < 0.01)
Fig. 3 Gene relative quantification in HPEpiC, PC3 and LNCaP cell lines. A AKT. B PI3K. C p53. D IL-1β. E IL-6. F IL-10. Bars colors in (A)–(E) represents studied groups: groups: Control group; 100% of UC-MSC; 10% of CBS. Symbols above bars represent statistical significance (*p < 0.05; **p < 0.01)CC BY · PubMed Central

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Metadata

Authors
Sousa, A., Coelho, P., Leite, F., Teixeira, C., Rocha, A. C., Santos, I., Baylina, P., Fernandes, R., Soares, R., Costa, R., & Gomes, A.
Affiliations
Universidade do Porto (PT)
Corresponding author
Gomes, Andreia
ORCID
Venue
Human Cell
Publisher
Springer Science and Business Media LLC
Published
2023-02-19
Volume
36
Issue
3
Pages
1160-1172
ISSN
  • 0914-7470 (ISSN-L)
  • 1749-0774
DOI
10.1007/s13577-023-00880-z
PMC
PMC10110723
OpenAlex
W4321366474
Cited by
15
References
2
Citations by year
2026: 2 · 2025: 4 · 2024: 7 · 2023: 2
FWCI
2.60
Type
journal article
Language
en
Open access
Licence
Locations
  • Human Cell (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT) (submittedVersion)
Funders
  • Bebé Vida - Ciências Para A Vida, S.A
  • Fundação para a Ciência e a Tecnologia (UID/BIM/0493/2020, 2021.06521.BD)
  • Instituto Politécnico do Porto (IPP4COVID/BI/02/2020)
  • Porto Research, Technology & Innovation Center (PORTIC/LabMi/BI/2021/03)
  • Universidade do Porto
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Mesenchymal stem cell research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Prostate Cancer Treatment and Research
  • Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
Keywords
prostate cancer, human umbilical cord mesenchymal stem cells, MSC secretome, cord blood serum, tumor progression, PI3K/Akt signaling, p53, interleukin genes, anti-inflammatory factors, cell proliferation, cell motility, PC-3 cells, LNCaP cells, cancer stem cells
MeSH terms
Secretome, Humans, Male, Prostate, Prostatic Neoplasms, Stem Cells, Umbilical Cord, Tumor Suppressor Protein p53, Phosphatidylinositol 3-Kinases, Serum, Proto-Oncogene Proteins c-akt

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CoChat. (2026). Citation check: Impact of umbilical cord mesenchymal stromal/stem cell secretome and cord blood serum in prostate cancer progression. https://app.cochat.ai/papers/10.1007/s13577-023-00880-z
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Sousa, A., Coelho, P., Leite, F., Teixeira, C., Rocha, A. C., Santos, I., Baylina, P., Fernandes, R., Soares, R., Costa, R., & Gomes, A. (2023). Impact of umbilical cord mesenchymal stromal/stem cell secretome and cord blood serum in prostate cancer progression. Human Cell. https://doi.org/10.1007/s13577-023-00880-z