Cited by 60Mitochondrial Function and PathologyPolyamine Metabolism and ApplicationsIon channel regulation and function

Regulation of Ca2+ transport in brain mitochondria. I. The mechanism of spermine enhancement of Ca2+ uptake and retention

Rottenberg, Hagai, Marbach, Miriam·1990·Biochimica et Biophysica Acta (BBA) - Bioenergetics

Abstract

Spermine enhances electrogenic Ca2+ uptake and inhibits Na(+)-independent Ca2+ efflux in rat brain mitochondria. As a result, Ca2+ retention by brain mitochondria increases greatly and the external free Ca2+ level at steady-state can be lowered to physiologically relevant concentrations. The stimulation of Ca2+ uptake by spermine is more pronounced at low concentrations of Ca2+, effectively lowering the apparent Km for Ca2+ uptake from 3 microM to 1.5 microM. However, the apparent Vmax is also increased. At low Ca2+ concentrations, Ca2+ uptake is diffusion-limited. Spermine strongly inhibits Ca2+ binding to anionic phospholipids and it is suggested that this increases the rate of surface diffusion which reduces the apparent Km for uptake. The same effect could inhibit the Na(+)-independent efflux if the rate of efflux is limited by Ca2+ dissociation from the efflux carrier. In brain mitochondria (but not in liver) the spermine effect depends on the presence of ADP. In a medium that contains physiological concentrations of Pi, Mg+, K+, ADP and spermine, brain mitochondria sequester Ca2+ down to 0.1 microM and below, depending on the matrix Ca2+ load. Moreover, brain mitochondria under the same conditions buffer the external medium at 0.4 microM, a concentration at which the set point becomes independent of the matrix Ca2+ content. Thus, mitochondria appear to be capable of modulating calcium oscillations in brain cells.

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Crossref · 10.1016/0005-2728(90)90009-s

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Authors
Rottenberg, H., & Marbach, M.
Affiliations
Hahnemann University Hospital (US)
Corresponding author
Rottenberg, Hagai
Venue
Biochimica et Biophysica Acta (BBA) - Bioenergetics
Publisher
Elsevier BV
Published
1990-03
Volume
1016
Issue
1
Pages
77-86
ISSN
0005-2728 (ISSN-L)
OpenAlex
W1981967527
Cited by
60
References
41
Citations by year
2026: 1 · 2025: 1 · 2021: 1 · 2019: 1 · 2018: 1 · 2015: 1 · 2014: 2
FWCI
2.95
Type
journal article
Language
en
Open access
closed
Locations
  • Biochimica et Biophysica Acta (BBA) - Bioenergetics (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institute on Alcohol Abuse and Alcoholism (AAO7238)
  • National Institute of General Medical Sciences (GM 28173)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Polyamine Metabolism and Applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
Keywords
calcium transport, brain mitochondria, spermine, calcium uptake, calcium retention, ADP, calcium buffering, calcium oscillations
MeSH terms
Animals, Biological Transport, Brain, Calcium, Kinetics, Male, Mitochondria, Models, Chemical, Rats, Inbred Strains, Spermine, Rats, In Vitro Techniques

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