GnRH-associated peptide (GAP) is cosecreted with GnRH into the hypophyseal portal blood of ovariectomized sheep
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The secretion of gonadotropin-releasing hormone (GnRH) and GnRH-associated peptide (GAP) into sheep hypothalamo-hypophyseal portal blood was investigated in ovariectomized ewes. GAP and GnRH were cosecreted into portal blood as determined in pooled 'peak' and 'trough' samples. The temporal pattern of GAP secretion into portal blood was coincidental with that of luteinizing hormone (LH) secretion into peripheral blood in three individual animals. The data provide the first evidence that GAP is a secretory product from mammalian hypothalamus and establish the temporal coexistence of the two peptides which appears to be of physiological significance in the regulation of pituitary function.
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No conservation was found at the level of the C-terminal portion of propeptides, which in vertebrates contains the GnRH-associated peptide (GAP).
“In our sequence analysis, we found no conservation at the level of the C-terminal portion of propeptides, which in vertebrates contains the GnRH-associated peptide (GAP) (55, 56) (SI Appendix, Fig. S1).”
From Corazonin signaling integrates energy homeostasis and lunar phase to regulate aspects of growth and sexual maturation in <i>Platynereis</i> (2020) · cites this paper as [55]
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Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific Activity
Hunter, W. M., & Greenwood, F. C. (1962) · Nature · doi:10.1038/194495a0 · Cited by 6,945 · see citing works
Clarke, I. J., & Cummins, J. T. (1982) · Endocrinology · doi:10.1210/endo-111-5-1737 · Cited by 921 · see citing works
Self-citationCARMEL, P. W., ARAKI, S., & FERIN, M. (1976) · Endocrinology · doi:10.1210/endo-99-1-243 · Cited by 482 · see citing works
Characterization of cDNA for precursor of human luteinizing hormone releasing hormone
Seeburg, P. H., & Adelman, J. P. (1984) · Nature · doi:10.1038/311666a0 · Cited by 441 · see citing works
Self-citationA prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormone
Nikolics, K., Mason, A. J., Szönyi, É., Ramachandran, J., & Seeburg, P. H. (1985) · Nature · doi:10.1038/316511a0 · Cited by 298 · see citing works
Self-citationClarke, I. J., Cummins, J. T., Findlay, J. K., Burman, K. J., & Doughton, B. W. (2008) · Neuroendocrinology · doi:10.1159/000123982 · Cited by 122 · see citing works
Self-citationNeuroendocrine molecular biology
(1987) · General and Comparative Endocrinology · doi:10.1016/0016-6480(87)90092-x · Cited by 104 · see citing works
PHILLIPS, H. S., Nikolics, K., Branton, D., & Seeburg, P. H. (1985) · Nature · doi:10.1038/316542a0 · Cited by 101 · see citing works
Self-citationBiosynthesis of LHRH: Inferences from immunocytochemical studies
King, J. C., & Anthony, E. L. P. (1983) · Peptides · doi:10.1016/0196-9781(83)90096-7 · Cited by 38 · see citing works
&NA; (1950) · The American Journal of the Medical Sciences · doi:10.1097/00000441-195022040-00042 · Cited by 33 · see citing works
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Research works printed but not linked: 3
- Wilson, J.D. and Foster, D.W., W.B. Saunders Co., Philadelphia, pp. 492-567
- Adelman, J.P., Mason, A.J., Hayflick, J.S. and Seeburg, P.H. (1986) Proc. Natl. Acad. Sci. USA 83-179-183
- Fink, G., Harmar, A.J. and McKerns, K.W., Plenum Press., New York, pp. 57-65
Cited by
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Neuroendrocrine regulation of prolactin release
Ben‐Jonathan, N., Arbogast, L. A., & Hyde, J. F. (1989) · Progress in Neurobiology · doi:10.1016/0301-0082(89)90008-7 · Cited by 277 · see citing works
Physiology of the Adult Gonadotropin-Releasing Hormone Neuronal Network
Herbison, A. E. (2014) · Elsevier eBooks · doi:10.1016/b978-0-12-397175-3.00011-9 · Cited by 255 · see citing works
Control of luteinizing hormone-releasing hormone pulse generation in nonhuman primates
Terasawa, E. (1995) · Cellular and Molecular Neurobiology · doi:10.1007/bf02069563 · Cited by 105 · see citing works
Sensory and Hormonal Control of Clutch Size in Birds
Haywood, S. (1993) · The Quarterly Review of Biology · doi:10.1086/417910 · Cited by 97 · see citing works
Roberts, J. L., Dutlow, C. M., Jakubowski, M., Blum, M., & Millar, R. P. (1989) · Molecular Brain Research · doi:10.1016/0169-328x(89)90046-6 · Cited by 90 · see citing works
Metabolism of Pro-Luteinizing Hormone-Releasing Hormone in Immortalized Hypothalamic Neurons*
Wetsel, W. C., Mellon, P. L., Weiner, R. I., & Negro-Vilar, A. F. (1991) · Endocrinology · doi:10.1210/endo-129-3-1584 · Cited by 76 · see citing works
Expression and Role of Gonadotropin-Releasing Hormone 2 and Its Receptor in Mammals
Desaulniers, A. T., Cederberg, R. A., Lents, C. A., & White, B. R. (2017) · Frontiers in Endocrinology · doi:10.3389/fendo.2017.00269 · Cited by 69 · see citing works
Andreatta, G., Broyart, C., Borghgraef, C., Vadiwala, K., Kozin, V., Polo, A., Bileck, A., Beets, I., Schoofs, L., Gerner, C., et al. (2020) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1910262116 · Cited by 61 · see citing works
Chu, Z., Takagi, H., & Moenter, S. M. (2010) · Journal of Neuroscience · doi:10.1523/jneurosci.1687-10.2010 · Cited by 49 · see citing works
Suwansa‐ard, S., Zhao, M., Thongbuakaew, T., Chansela, P., Ventura, T., Cummins, S. F., & Sobhon, P. (2016) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2016.07.008 · Cited by 42 · see citing works
Yu, W. H., Seeburg, P. H., Nikolics, K., & McCann, S. M. (1988) · Endocrinology · doi:10.1210/endo-123-1-390 · Cited by 39 · see citing works
Self-citationGaillard, A.-L., Tay, B.-H., Sirkin, D. I. P., Lafont, A.-G., de Flori, C., Vissio, P. G., Mazan, S., Dufour, S., Venkatesh, B., & Tostivint, H. (2018) · Frontiers in Neuroscience · doi:10.3389/fnins.2018.00607 · Cited by 37 · see citing works
Wetsel, W. C., & Negro-Vilar, A. F. (1989) · Endocrinology · doi:10.1210/endo-125-1-538 · Cited by 30 · see citing works
Wetsel, W. C., Culler, M. D., Johnston, C. A., & Negro-Vilar, A. F. (1988) · Molecular Endocrinology · doi:10.1210/mend-2-1-22 · Cited by 26 · see citing works
Yu, W. H., Millar, R. P., Milton, S. C. F., del Milton, R. C., & McCann, S. M. (1990) · Brain Research Bulletin · doi:10.1016/0361-9230(90)90182-y · Cited by 25 · see citing works
Lea, R. W., & Sharp, P. J. (1989) · Hormones and Behavior · doi:10.1016/0018-506x(89)90067-6 · Cited by 23 · see citing works
Andersen, A. C., Danger, J., Fasolo, A., Kah, O., Tonon, M., & Vaudry, H. (1988) · The Journal of Comparative Neurology · doi:10.1002/cne.902730208 · Cited by 23 · see citing works
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Merchenthaler, I., Culler, M., Petrusz, P., Flerk�, B., & Negro-Vilar, A. (1989) · Cell and Tissue Research · doi:10.1007/bf00229060 · Cited by 22 · see citing works
Wetsel, W. C., & Srinivasan, S. (2002) · Progress in brain research · doi:10.1016/s0079-6123(02)41096-5 · Cited by 21 · see citing works
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Fraser, H. M. (1988) · Baillière s Clinical Obstetrics and Gynaecology · doi:10.1016/s0950-3552(88)80050-6 · Cited by 21 · see citing works
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Clarke, I. J., Campbell, R. E., Smith, J. T., Prévot, V., & Wray, S. (2012) · Elsevier eBooks · doi:10.1016/b978-0-12-375097-6.10009-5 · Cited by 20 · see citing works
Self-citationThe timing of puberty (oocyte quality and management)
Duittoz, A. H., Tillet, Y., Bourhis, D. L., & Schibler, L. (2016) · Animal Reproduction · doi:10.21451/1984-3143-ar874 · Cited by 19 · see citing works
Bloch, B., Gaillard, R. C., Culler, M. D., & Negro-Vilar, A. F. (1992) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/jc.74.1.135 · Cited by 19 · see citing works
Kerrigan, J. R., Dalkin, A. C., Haisenleder, D. J., Yasin, M., & Marshall, J. C. (1993) · Endocrinology · doi:10.1210/en.133.5.2071 · Cited by 18 · see citing works
Pohl, C. R., Weiner, R. I., & Smith, M. S. (1988) · Endocrinology · doi:10.1210/endo-123-3-1591 · Cited by 18 · see citing works
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Clifton, D. K., & Steiner, R. A. (2009) · Elsevier eBooks · doi:10.1016/b978-1-4160-4907-4.00001-2 · Cited by 17 · see citing works
Terasawa, E. (1994) · Methods in neurosciences · doi:10.1016/b978-0-12-185289-4.50016-4 · Cited by 17 · see citing works
Kereilwe, O., & Kadokawa, H. (2020) · Domestic Animal Endocrinology · doi:10.1016/j.domaniend.2019.106432 · Cited by 16 · see citing works
Feng, K., Luo, H., Hou, M., Li, Y., Chen, J., Zhu, Z., & Hu, W. (2018) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2018.05.021 · Cited by 15 · see citing works
The pro-LHRH system of the rat brain. effects of changes in the endocrine background
Merchenthaler, I. J., Culler, M. D., Negro-Vilar, A. F., Petrusz, P., & Flerkó, B. (1988) · Brain Research Bulletin · doi:10.1016/0361-9230(88)90082-2 · Cited by 15 · see citing works
Pérez Sirkin, D. I., Lafont, A.-G., Kamech, N., Somoza, G. M., Vissio, P. G., & Dufour, S. (2017) · Frontiers in Endocrinology · doi:10.3389/fendo.2017.00207 · Cited by 14 · see citing works
The Salmon GnRH Encoding Gene in Teleost Fish
Andersen, Ø., & Klungland, H. (1993) · International review of cytology · doi:10.1016/s0074-7696(08)60768-8 · Cited by 14 · see citing works
Vacher, P., Mariot, P., Dufy-Barbe, L., Nikolics, K., SEEBURG, P. H., Kerdelhué, B., & DUFY, B. (1991) · Endocrinology · doi:10.1210/endo-128-1-285 · Cited by 14 · see citing works
Self-citationMolecular biology of the regulation of hypothalamic hormones
Rondeel, J. M. M., & JACKSON, I. M. D. (1993) · Journal of Endocrinological Investigation · doi:10.1007/bf03344952 · Cited by 13 · see citing works
Kerrigan, J. R., Yasin, M., Haisenleder, D. J., Dalkin, A. C., & Marshall, J. C. (1995) · Biology of Reproduction · doi:10.1095/biolreprod53.1.1 · Cited by 12 · see citing works
Valênça, M. M., Wetsel, W. C., Culler, M. D., & Negro-Vilar, A. F. (1988) · Molecular and Cellular Endocrinology · doi:10.1016/0303-7207(88)90095-0 · Cited by 11 · see citing works
The gene encoding GnRH and its associated peptide GAP: Some insights into hypogonadism
SEEBURG, P. H., Mason, A. J., Young, W. S., Stewart, T. A., & Nikolics, K. (1989) · Journal of Steroid Biochemistry · doi:10.1016/0022-4731(89)90479-2 · Cited by 10 · see citing works
Self-citationWańkowska, M., & Polkowska, J. (2009) · Journal of Chemical Neuroanatomy · doi:10.1016/j.jchemneu.2009.05.003 · Cited by 9 · see citing works
Prolactin Releasing and Inhibiting Factors in the Posterior Pituitary
Ben‐Jonathan, N. (1990) · Neuroendocrine perspectives · doi:10.1007/978-1-4612-3446-3_1 · Cited by 9 · see citing works
Combined antibody-high-performance liquid chromatography approach to assess prohormone processing
Wetsel, W. C., & Negro-Vilar, A. F. (1989) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(89)68040-8 · Cited by 9 · see citing works
Abe, J., Okamura, H., Ibata, Y., Motoyama, A., Wakabayashi, I., LING, N. C., & Paull, W. K. (1990) · Histochemistry and Cell Biology · doi:10.1007/bf02440178 · Cited by 5 · see citing works
Dufy-Barbe, L., Bresson‐Bepoldin, L., Vacher, P., Odessa, M. F., Galli, F. S., Rivel, J., & Guerin, J. B. (1993) · Molecular and Cellular Endocrinology · doi:10.1016/0303-7207(93)90030-n · Cited by 4 · see citing works
Blake, C. A., Campbell, G. T., Mascagni, F., Culler, M. D., & Negro-Vilar, A. F. (1993) · Biology of Reproduction · doi:10.1095/biolreprod49.5.965 · Cited by 4 · see citing works
Posttranslational processing of progonadotropin-releasing hormone (PROGnRH) in ewes
Connor, H. C., & Moss, G. E. (1994) · Domestic Animal Endocrinology · doi:10.1016/0739-7240(94)90003-5 · Cited by 4 · see citing works
Gobert, A., Guibert, B., Lenoir, V., Kerdelhué, B., & Leviel, V. (1992) · Journal of Neuroscience Research · doi:10.1002/jnr.490310218 · Cited by 4 · see citing works
Alpha-synuclein expression in anterior pituitary cells of aged cattle
Niyonzima, Y. B., Wanjiru, D. K., Asato, Y., & Kadokawa, H. (2025) · Domestic Animal Endocrinology · doi:10.1016/j.domaniend.2025.106936 · Cited by 3 · see citing works
Wańkowska, M., & Polkowska, J. (2010) · Folia Histochemica et Cytobiologica · doi:10.2478/v10042-008-0108-2 · Cited by 1 · see citing works
OPINIONS A brief overview of the process of the elucidation of GnRH structure (1971)
Kochman, K. (2014) · Biotechnologia · doi:10.5114/bta.2012.46590
Rodin, D. A. (1991) · UCL Discovery (University College London)
Conservation and plasticity in avian prepro-GnRH-I reflect a modular evolutionary architecture
Cervantes-Valencia, M. E., van der Goes, T. I. F., González-Villalva, A. E., Lemus, M. R., López-Valdéz, N., & Chávez-Maya, F. (2026) · General and Comparative Endocrinology · doi:10.1016/j.ygcen.2026.114998
- Authors
- Clarke, I. J., Cummins, J. T., Karsch, F. J., Seeburg, P. H., & Nikolics, K.
- Affiliations
- St Vincent's Hospital Melbourne (AU)
- University of Michigan (US)
- Genentech (US)
- ORCID
- Clarke, I.J.
- Venue
- Biochemical and Biophysical Research Communications
- Publisher
- Elsevier BV
- Published
- 1987-03
- Volume
- 143
- Issue
- 2
- Pages
- 665-671
- ISSN
- 0006-291X (ISSN-L)
- OpenAlex
- W1975607182
- Cited by
- 52
- References
- 10
- Citations by year
- 2026: 1 · 2025: 1 · 2020: 1 · 2019: 2 · 2018: 2 · 2017: 2 · 2016: 2 · 2014: 2 · 2012: 2
- FWCI
- 7.05
- Type
- journal article
- Language
- en
- Open access
- green
- hdl.handle.net
- Locations
- Biochemical and Biophysical Research Communications (publishedVersion)
- PubMed (publishedVersion)
- Deep Blue (University of Michigan) (submittedVersion)
- Topics
- Health Sciences › Medicine › Reproductive Medicine › Hypothalamic control of reproductive hormones
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Neuropeptides and Animal Physiology
- Social Sciences › Psychology › Social Psychology › Neuroendocrine regulation and behavior
- Keywords
- GnRH-associated peptide, gonadotropin-releasing hormone, ovariectomized ewes, luteinizing hormone, pituitary regulation, sheep
- MeSH terms
- Animals, Female, Hypothalamo-Hypophyseal System, Hypothalamus, Luteinizing Hormone, Gonadotropin-Releasing Hormone, Ovariectomy, Periodicity, Protein Precursors, Sheep
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