Cited by 43Nicotinic Acetylcholine Receptors StudyCircadian rhythm and melatoninEstrogen and related hormone effects

Effects of gonadal steroids on the in vivo binding of [125I]α-bungarotoxin to the suprachiasmatic nucleus

Miller, Marilyn M., Silver, Jerry, Billiar, Reinhart B.·1984·Brain Research

Abstract

The radioligand [125I]alpha-bungarotoxin (alpha-BTX) has been used to test receptor binding to putative nicotinic cholinergic receptors in the hypothalamus. Using light microscopic autoradiography following third ventricular infusion of the radioligand we have previously demonstrated that in normally cycling rats and in normal males, the suprachiasmatic nucleus (SCN) consistently binds the alpha-neurotoxin. In chronically (5 weeks) oophorectomized female, binding of [125I]alpha-BTX to the SCN is markedly diminished. The present series of experiments were designed to test the effects of gonadal steroids on the binding of [125I]alpha-BTX to the SCN. We first tested whether or not estradiol administered to ovariectomized females could duplicate the presence of the ovary. In females ovariectomized and immediately provided with a constant dose of estradiol-17 beta (E2)--1.0 cm silastic capsules for 5 weeks, (n = 4), the binding of the neurotoxin to the SCN was maintained. In females ovariectomized for 3 weeks and replaced with E2 for 2 weeks (n = 4), the binding of [125I]alpha-BTX to the SCN was restored. In chronically (4 weeks) ovariectomized females receiving E2 for 6 days (n = 2), the binding of the neurotoxin was partially restored. We next tested the effect of chronic (5 weeks) castration (n = 100) and observed that binding of [125I]alpha-BTX to the SCN of castrated males was like that of intact male controls (n = 6).(ABSTRACT TRUNCATED AT 250 WORDS)

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Crossref · 10.1016/0006-8993(84)90736-4

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  • In females, gonadal hormones can increase the sensitivity of the SCN to cholinergic inputs.

    “In females gonadal hormones can also increase the sensitivity of the SCN to cholinergic inputs (ACh; [96, 117, 118]), which are thought to potentially mediate the influence of cognition (sustained attention) on chronotype [63].”

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Authors
Miller, M. M., Silver, J., & Billiar, R. B.
Affiliations
Case Western Reserve University (US)
Venue
Brain Research
Publisher
Elsevier BV
Published
1984-01
Volume
290
Issue
1
Pages
67-75
ISSN
0006-8993 (ISSN-L)
OpenAlex
W1975084142
Cited by
43
References
54
Citations by year
2024: 2 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 1 · 2016: 2 · 2015: 1 · 2014: 1 · 2012: 1
FWCI
1.06
Type
journal article
Language
en
Open access
closed
Locations
  • Brain Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
National Institutes of Health
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
  • Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
Keywords
suprachiasmatic nucleus, sex steroids, estradiol, testosterone, oophorectomy, castration, nicotinic acetylcholine receptors, alpha-bungarotoxin binding, sex differences, neonatal androgenization, circadian rhythm, rats, third ventricle infusion, autoradiography
MeSH terms
Animals, Animals, Newborn, Bungarotoxins, Castration, Estradiol, Female, Male, Rats, Inbred Strains, Gonadal Steroid Hormones, Suprachiasmatic Nucleus, Testosterone, Time Factors, Rats

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