Effects of gonadal steroids on the in vivo binding of [125I]α-bungarotoxin to the suprachiasmatic nucleus
Abstract
The radioligand [125I]alpha-bungarotoxin (alpha-BTX) has been used to test receptor binding to putative nicotinic cholinergic receptors in the hypothalamus. Using light microscopic autoradiography following third ventricular infusion of the radioligand we have previously demonstrated that in normally cycling rats and in normal males, the suprachiasmatic nucleus (SCN) consistently binds the alpha-neurotoxin. In chronically (5 weeks) oophorectomized female, binding of [125I]alpha-BTX to the SCN is markedly diminished. The present series of experiments were designed to test the effects of gonadal steroids on the binding of [125I]alpha-BTX to the SCN. We first tested whether or not estradiol administered to ovariectomized females could duplicate the presence of the ovary. In females ovariectomized and immediately provided with a constant dose of estradiol-17 beta (E2)--1.0 cm silastic capsules for 5 weeks, (n = 4), the binding of the neurotoxin to the SCN was maintained. In females ovariectomized for 3 weeks and replaced with E2 for 2 weeks (n = 4), the binding of [125I]alpha-BTX to the SCN was restored. In chronically (4 weeks) ovariectomized females receiving E2 for 6 days (n = 2), the binding of the neurotoxin was partially restored. We next tested the effect of chronic (5 weeks) castration (n = 100) and observed that binding of [125I]alpha-BTX to the SCN of castrated males was like that of intact male controls (n = 6).(ABSTRACT TRUNCATED AT 250 WORDS)
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In females, gonadal hormones can increase the sensitivity of the SCN to cholinergic inputs.
“In females gonadal hormones can also increase the sensitivity of the SCN to cholinergic inputs (ACh; [96, 117, 118]), which are thought to potentially mediate the influence of cognition (sustained attention) on chronotype [63].”
From The neuroendocrine control of the circadian system: Adolescent chronotype (2012) · cites this paper as [118]
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- Authors
- Miller, M. M., Silver, J., & Billiar, R. B.
- Affiliations
- Case Western Reserve University (US)
- ORCID
- Silver, Jerry
- Venue
- Brain Research
- Publisher
- Elsevier BV
- Published
- 1984-01
- Volume
- 290
- Issue
- 1
- Pages
- 67-75
- ISSN
- 0006-8993 (ISSN-L)
- OpenAlex
- W1975084142
- Cited by
- 43
- References
- 54
- Citations by year
- 2024: 2 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 1 · 2016: 2 · 2015: 1 · 2014: 1 · 2012: 1
- FWCI
- 1.06
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Brain Research (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nicotinic Acetylcholine Receptors Study
- Life Sciences › Neuroscience › Endocrine and Autonomic Systems › Circadian rhythm and melatonin
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Keywords
- suprachiasmatic nucleus, sex steroids, estradiol, testosterone, oophorectomy, castration, nicotinic acetylcholine receptors, alpha-bungarotoxin binding, sex differences, neonatal androgenization, circadian rhythm, rats, third ventricle infusion, autoradiography
- MeSH terms
- Animals, Animals, Newborn, Bungarotoxins, Castration, Estradiol, Female, Male, Rats, Inbred Strains, Gonadal Steroid Hormones, Suprachiasmatic Nucleus, Testosterone, Time Factors, Rats
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