Inhibition of the Fenton reaction by the protein caeruloplasmin and other copper complexes. Assessment of ferroxidase and radical scavenging activities
Abstract
The copper-containing protein caeruloplasmin is an important biological extracellular protein. By catalysing the oxidation of ferrous ions to the ferric state (ferroxidase activity) it can inhibit lipid peroxidation and the Fenton reaction. This activity is readily destroyed by heat-denaturation. When a ferric-EDTA complex is added to hydrogen peroxide, OH X radicals are formed in a reaction inhibitable by superoxide dismutase (SOD). This reaction is also inhibited by caeruloplasmin both before and after heat-denaturation, suggesting a non-catalytic scavenging role for the protein. A combination of ferroxidase and radical scavenging activities in fluids containing iron complexes and hydrogen peroxide, but no SOD or catalase, would make caeruloplasmin an important extracellular antioxidant.
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Proteins like ceruloplasmin, transferrin, or lactoferrin limit the Fenton reaction.
“Proteins like ceruloplasmin, transferrin, or lactoferrin limit the reaction (388, 1020).”
From Sources of Vascular Nitric Oxide and Reactive Oxygen Species and Their Regulation (2019) · cites this paper as [388]
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- Authors
- Gutteridge, J. M. C.
- Affiliations
- National Institute for Biological Standards and Control (GB)
- Corresponding author
- Gutteridge, John M.C.
- Venue
- Chemico-Biological Interactions
- Publisher
- Elsevier BV
- Published
- 1985-12
- Volume
- 56
- Issue
- 1
- Pages
- 113-120
- ISSN
- 0009-2797 (ISSN-L)
- OpenAlex
- W2005031071
- Cited by
- 85
- References
- 22
- Citations by year
- 2023: 1 · 2022: 1 · 2021: 3 · 2020: 1 · 2018: 4 · 2017: 1 · 2015: 3 · 2014: 2
- FWCI
- 0.99
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Chemico-Biological Interactions (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
- Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
- Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
- Keywords
- ceruloplasmin, Fenton reaction, ferroxidase activity, free radical scavenging, copper complexes, lipid peroxidation inhibition, hydrogen peroxide, superoxide dismutase, thermal denaturation, antioxidant proteins, hydroxyl radicals
- MeSH terms
- Ceruloplasmin, Copper, Deoxyribose, Edetic Acid, Ferric Compounds, Ferrous Compounds, Free Radicals, Hydrogen Peroxide, Hydroxides, Iron, Oxidation-Reduction, Superoxides, Superoxide Dismutase, Hydroxyl Radical
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
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