Cited by 85Metal-Catalyzed Oxygenation MechanismsAntioxidant Activity and Oxidative StressFree Radicals and Antioxidants

Inhibition of the Fenton reaction by the protein caeruloplasmin and other copper complexes. Assessment of ferroxidase and radical scavenging activities

Gutteridge, John M.C.·1985·Chemico-Biological Interactions

Abstract

The copper-containing protein caeruloplasmin is an important biological extracellular protein. By catalysing the oxidation of ferrous ions to the ferric state (ferroxidase activity) it can inhibit lipid peroxidation and the Fenton reaction. This activity is readily destroyed by heat-denaturation. When a ferric-EDTA complex is added to hydrogen peroxide, OH X radicals are formed in a reaction inhibitable by superoxide dismutase (SOD). This reaction is also inhibited by caeruloplasmin both before and after heat-denaturation, suggesting a non-catalytic scavenging role for the protein. A combination of ferroxidase and radical scavenging activities in fluids containing iron complexes and hydrogen peroxide, but no SOD or catalase, would make caeruloplasmin an important extracellular antioxidant.

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Crossref · 10.1016/0009-2797(85)90043-2

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22 of 22 references verified0 citation errors · 0 not checkable

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Authors
Gutteridge, J. M. C.
Affiliations
National Institute for Biological Standards and Control (GB)
Corresponding author
Gutteridge, John M.C.
Venue
Chemico-Biological Interactions
Publisher
Elsevier BV
Published
1985-12
Volume
56
Issue
1
Pages
113-120
ISSN
0009-2797 (ISSN-L)
OpenAlex
W2005031071
Cited by
85
References
22
Citations by year
2023: 1 · 2022: 1 · 2021: 3 · 2020: 1 · 2018: 4 · 2017: 1 · 2015: 3 · 2014: 2
FWCI
0.99
Type
journal article
Language
en
Open access
closed
Locations
  • Chemico-Biological Interactions (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Physical Sciences › Chemistry › Inorganic Chemistry › Metal-Catalyzed Oxygenation Mechanisms
  • Health Sciences › Medicine › Biochemistry › Antioxidant Activity and Oxidative Stress
  • Physical Sciences › Chemistry › Organic Chemistry › Free Radicals and Antioxidants
Keywords
ceruloplasmin, Fenton reaction, ferroxidase activity, free radical scavenging, copper complexes, lipid peroxidation inhibition, hydrogen peroxide, superoxide dismutase, thermal denaturation, antioxidant proteins, hydroxyl radicals
MeSH terms
Ceruloplasmin, Copper, Deoxyribose, Edetic Acid, Ferric Compounds, Ferrous Compounds, Free Radicals, Hydrogen Peroxide, Hydroxides, Iron, Oxidation-Reduction, Superoxides, Superoxide Dismutase, Hydroxyl Radical

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