A simple immunotechnique for the determination of serum concentration of apolipoprotein E
Abstract
Immunoturbidimetric assay for the determination of serum apolipoprotein E was developed on the Cobas-BIO centrifugal analyzer using commercially available antisera and reference sera. Within-run imprecision was 2.9% while day-to-day imprecision was 7.9%. Addition of up to 50 g/l hemoglobin or 0.15 g/l bilirubin to sera did not interfere with the determination of this apolipoprotein. Apolipoprotein E concentration was measured in sera from 40 normolipidemic and hyperlipidemic subjects by this immunoturbidimetric method (dependent variable) and radial immunodiffusion assay (independent variable). A slope of 0.90, an intercept of -7.9, and a correlation coefficient of 0.94 were obtained. Reference range for apolipoprotein E was established using sera from 100 normolipidemic subjects. The mean + 1 SD for apolipoprotein E levels of males and females were 36 + 13 mg/l and 29 + 8.4 mg/l, respectively. The distribution of apolipoprotein E among the various lipoprotein fractions of normals was significantly different than that of hypertriglyceridemic subjects.
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Hypertriglyceridemic patients have higher plasma levels of apoE compared with normal control subjects.
“Hypertriglyceridemic patients have higher plasma levels of apoE compared with normal control subjects; this could probably be explained by the finding, that increased levels of apoE stimulate VLDL triglyceride production in the liver and impairs lipoprotein lipase-mediated lipolysis, thus leading to hypertriglyceridemia [56,65].”
From Plasma levels of apolipoprotein E, APOE genotype and risk of dementia and ischemic heart disease: A review (2016) · cites this paper as [56]
Hypertriglyceridaemic patients have higher plasma levels of apoE compared with normal control subjects.
“Hypertriglyceridaemic patients have higher plasma levels of apoE compared with normal control subjects”
Increased levels of apoE stimulate very low-density lipoprotein triglyceride production in the liver and impair lipoprotein lipase-mediated lipolysis, leading to hypertriglyceridaemia.
“increased levels of apoE stimulate very low-density lipoprotein triglyceride production in the liver and impairs lipoprotein lipase-mediated lipolysis, thus leading to hypertriglyceridaemia.42,43”
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Rasmussen, K. L. (2016) · Atherosclerosis · doi:10.1016/j.atherosclerosis.2016.10.037 · Cited by 125 · see citing works
Rasmussen, K. L., Tybjærg-Hansen, A., Nordestgaard, B. G., & Frikke-Schmidt, R. (2019) · European Heart Journal · doi:10.1093/eurheartj/ehz402 · Cited by 86 · see citing works
- Authors
- Rifai, N., & Silverman, L. M.
- Affiliations
- University of North Carolina at Chapel Hill (US)
- Corresponding author
- Rifai, Nader
- ORCID
- Rifai, Nader
- Venue
- Clinica Chimica Acta
- Publisher
- Elsevier BV
- Published
- 1987-03
- Volume
- 163
- Issue
- 2
- Pages
- 207-213
- ISSN
- 0009-8981 (ISSN-L)
- OpenAlex
- W2091400416
- Cited by
- 23
- References
- 0
- Citations by year
- 2022: 1 · 2021: 1 · 2019: 1 · 2016: 2 · 2014: 1 · 2012: 1
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Clinica Chimica Acta (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Lipoproteins and Cardiovascular Health
- Health Sciences › Medicine › Physiology › Clinical Laboratory Practices and Quality Control
- Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Lipid metabolism and disorders
- Keywords
- apolipoprotein E, immunoturbidimetric assay, hyperlipidemia, hypertriglyceridemia, radial immunodiffusion, reference values, lipoprotein fractions, assay interference
- MeSH terms
- Adolescent, Adult, Apolipoproteins E, Female, Humans, Hyperlipidemias, Immunologic Techniques, Lipoproteins, Male, Middle Aged, Nephelometry and Turbidimetry, Sex Factors, Triglycerides
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