The DOI matches its Crossref record; 38 of the 38 references it cites were found in a registry or an index.
The differential appearance of neurofilament triplet polypeptides in the developing rat optic nerve
Abstract
The ontogenetic appearance of the individual triplet polypeptides that comprise mammalian neurofilaments was studied in the developing rat optic nerve. Triton-insoluble cytoskeletal preparations from the optic nerves of rats of postnatal ages 1 Day (P1), 6 days (P6), 10 days (P10), 20 days (P20), and 3 months (adult) were analyzed for protein composition by one and two-dimensional gel electrophoresis. Results indicate that at P1, both the 150- and 68-kDa neurofilament subunit proteins are present. The 200-kDa subunit first becomes discernible at P20, but, at this age, it is still present in considerably less quantity than in the adult. Immunocytochemical verification of the presence of neurofilament protein was accomplished by staining tissue sections with specific antibodies against the 150- and the 68-kDa neurofilament subunits using the peroxidase-antiperoxidase technique. Results of the morphological analyses have shown that neurofilaments are not present in quantity until P10, which coincides with the time when the 68-kDa subunit increases in quantity by one dimensional gel analysis. Thus, the 150- and 68-kDa subunits can be detected prior to the appearance of neurofilaments, and the 200-kDa protein is not observed until sometime later. The potential physiological significance of the differential subunit transport is discussed with respect to neuronal differentiation in the developing mammalian CNS.
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“In this regard, microtubules are apt to be especially important because they arise early in neuronal development, usually much before neurofilaments (Pachter and Liem, 1984;Peters and Vaughn, 1967;Shaw et al., 1985), and because they have been implicated in a variety of cellular events, including the outgrowth of neuronal processes and the transport of materials within axons and dendrites.”
From Immunocytochemical localization of tubulin and microtubule-associated protein 2 during the development of hippocampal neurons in culture (1986) · cites this paper as Pachter and Liem, 1984
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Cites
PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT
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Differential expression of neurofilament triplet proteins in brain development
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Axonal Growth-Associated Proteins
Skene, J. H. P. (1989) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.12.030189.001015 · Cited by 1,145
The avidin-biotin complex in bioanalytical applications
Wilchek, M., & Bayer, E. A. (1988) · Analytical Biochemistry · doi:10.1016/0003-2697(88)90120-0 · Cited by 895
Neurofilaments and Neurofilament Proteins in Health and DiseasePreprint
Yuan, A., Rao, M. V., Veeranna, & Nixon, R. A. (2017) · Cold Spring Harbor Perspectives in Biology · doi:10.1101/cshperspect.a018309 · Cited by 741
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Carden, M. J., Trojanowski, J. Q., Schlaepfer, W. W., & Lee, V. (1987) · Journal of Neuroscience · doi:10.1523/jneurosci.07-11-03489.1987 · Cited by 499
Metadata
- Authors
- Pachter, J. S., & Liem, R. K. H.
- Affiliations
- New York University (US)
- Corresponding author
- Liem, Ronald K.H.
- Venue
- Developmental Biology
- Publisher
- Elsevier BV
- Published
- 1984-05
- Volume
- 103
- Issue
- 1
- Pages
- 200-210
- ISSN
- 0012-1606 (ISSN-L)
- OpenAlex
- W1990069940
- Cited by
- 175
- References
- 38
- Citations by year
- 2024: 2 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 1 · 2017: 3 · 2015: 2 · 2012: 2
- FWCI
- 6.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Developmental Biology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Skin and Cellular Biology Research
- Life Sciences › Neuroscience › Developmental Neuroscience › Neurogenesis and neuroplasticity mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
- Keywords
- neurofilaments, neurofilament subunits, optic nerve development, postnatal development, cytoskeleton, neuronal differentiation, central nervous system development, rats
- MeSH terms
- Aging, Animals, Animals, Newborn, Cytoskeleton, Electrophoresis, Polyacrylamide Gel, Immunoassay, Intermediate Filament Proteins, Microscopy, Electron, Microtubules, Molecular Weight, Optic Nerve, Rats, Inbred Strains, Neurofilament Proteins, Rats
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