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Sequence analysis of phosphoserine‐containing peptides: Modification for picomolar sensitivity
Open accessAbstract
Sequencing of phosphoserine‐containing peptides yields normally no identifiable PTH‐derivatives at those positions where phosphoserine is located. Here a new method is described which allows identification of the position of phosphoserine by chemical modification just before sequence analysis. In a one‐step microbatch reaction, phosphoserine present in the intact peptide can be transformed quantitatively into stable derivatives such as β‐methylaminoalanine (MAA), S‐ethanolcysteine or S‐ethylcysteine. These derivatives are detectable during microsequencing with less than 100 pmol peptide using an Applied Biosystems gas‐phase sequencer equipped with an on‐line PTH amino acid analyzer.
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Cited by
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Lüscher, B., Christenson, E., Litchfield, D. W., Krebs, E. G., & Eisenman, R. N. (1990) · Nature · doi:10.1038/344517a0 · Cited by 344
Goshe, M. B., Conrads, T. P., Panisko, E. A., Angell, N. H., Veenstra, T. D., & Smith, R. D. (2001) · Analytical Chemistry · doi:10.1021/ac010081x · Cited by 318
Phosphopeptide Analysis by Matrix-Assisted Laser Desorption Time-of-Flight Mass Spectrometry
Annan, R. S., & Carr, S. A. (1996) · Analytical Chemistry · doi:10.1021/ac960221g · Cited by 310
Fiol, C. J., Williams, J. S., Chou, C. H., Wang, Q. M., Roach, P. J., & Andrisani, O. M. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)31619-3 · Cited by 277
Byford, M. F. (1991) · Biochemical Journal · doi:10.1042/bj2800261 · Cited by 79
Metadata
- Authors
- Meyer, H. E., Hoffmann-Posorske, E., Korte, H., & Heilmeyer, L. M. G.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1986-08-11
- Volume
- 204
- Issue
- 1
- Pages
- 61-66
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1555247363
- Cited by
- 260
- References
- 0
- FWCI
- 10.82
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Meyer, H. E., Hoffmann-Posorske, E., Korte, H., & Heilmeyer, L. M. G. (1986). Sequence analysis of phosphoserine‐containing peptides: Modification for picomolar sensitivity. FEBS Letters. https://doi.org/10.1016/0014-5793(86)81388-6
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