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Complete amino acid sequence of human intestinal aminopeptidase N as deduced from cloned cDNA
Open accessAbstract
The complete primary structure (967 amino acids) of an intestinal human aminopeptidase N (EC 3.4.11.2) was deduced from the sequence of a cDNA clone. Aminopeptidase N is anchored to the microvillar membrane via an uncleaved signal for membrane insertion. A domain constituting amino acid 250–555 positioned within the catalytic domain shows very clear homology to E. coli aminopeptidase N and contains Zn 2+ ligands. Therefore these residues are part of the active site. However, no homology of the anchor/junctional peptide domain is found suggesting that the juxta‐ and intra‐membraneous parts of the molecule have been added/preserved during development. It is speculated that this part carries the apical address.
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Kozak, M. (1986) · Cell · doi:10.1016/0092-8674(86)90762-2 · Cited by 4,741
Cited by
Predicting the orientation of eukaryotic membrane-spanning proteins.
Hartmann, E., Rapoport, T. A., & Lodish, H. F. (1989) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.86.15.5786 · Cited by 584
A unique signature identifies a family of zinc‐dependent metallopeptidases
Jongeneel, C. V., Bouvier, J., & Bairoch, A. (1989) · FEBS Letters · doi:10.1016/0014-5793(89)80471-5 · Cited by 461
Tresnan, D. B., Levis, R., & Holmes, K. V. (1996) · Journal of Virology · doi:10.1128/jvi.70.12.8669-8674.1996 · Cited by 264
Metadata
- Authors
- Olsen, J., Cowell, G. M., Kønigshøfer, E., Danielsen, E. M., Møller, J., Laustsen, L., Hansen, O. C., Welinder, K. G., Engberg, J., Hunziker, W., Spiesst, M., Sjöström, H., & Norén, O.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1988-10-10
- Volume
- 238
- Issue
- 2
- Pages
- 307-314
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2056675361
- Cited by
- 245
- References
- 1
- FWCI
- 7.58
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Olsen, J., Cowell, G. M., Kønigshøfer, E., Danielsen, E. M., Møller, J., Laustsen, L., Hansen, O. C., Welinder, K. G., Engberg, J., Hunziker, W., Spiesst, M., Sjöström, H., & Norén, O. (1988). Complete amino acid sequence of human intestinal aminopeptidase N as deduced from cloned cDNA. FEBS Letters. https://doi.org/10.1016/0014-5793(88)80502-7
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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