Simultaneous recordings of glucose dependent electrical activity and ATP‐regulated K+‐currents in isolated mouse pancreatic β‐cells
Abstract
Membrane potential and membrane currents were recorded from single mouse pancreatic beta-cells using the perforated patch whole-cell recording technique at 30 degrees C. Single beta-cells maintained in primary tissue culture exhibited glucose-dependent electrical activity similar to that reported for freshly isolated intact islets. The resting input conductance (5.1 +/- 0.9 nS) was determined by ATP-regulated K+ (KATP) channels as it was blocked by 1 mM tolbutamide. 8 mM glucose decreased the input conductance by 80%. The input conductance at -70 mV was of a similar value during the plateau phase and during the silent phase of electrical activity in 8 mM glucose. This suggests that oscillations of KATP channel activity do not underlie the slow waves.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
In whole-cell recordings, the maximum KATP conductance when ATP has washed out is around 2 nS/pF; in perforated patch it is ~1 nS/pF in single β-cells exposed to glucose-free solution; in β-cells within freshly isolated islets it ranges between 0.25 and 0.4 nS/pF.
“In whole-cell recordings, the maximum KATP conductance, measured when ATP has washed out of the cell, is around 2 nS/pF (573). When measured in the perforated patch configuration (where cytosolic ATP levels are undisturbed) it is ~1 nS/pF in single β-cells exposed to glucose-free solution (584, 631). In β-cells within freshly isolated pancreatic islets, it is significantly lower and ranges between”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [631]
The membrane conductance of β-cells in intact islets at 20 mM glucose is about 1 nS, 10-fold higher than that observed in dispersed single β-cells.
“The membrane conductance of β-cells in intact islets (measured in perforated patch recordings at 20 mM glucose as outlined in Figure 11A) is ~1 nS (221), 10-fold higher than that observed in dispersed single β-cells (631).”
From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [631]
Cited by
Sorted by citations
Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men
Rorsman, P., & Ashcroft, F. M. (2018) · Physiological Reviews · doi:10.1152/physrev.00008.2017 · Cited by 793 · see citing works
Self-citationRegulation of Insulin Secretion in Human Pancreatic Islets
Rorsman, P., & Braun, M. (2013) · Annual Review of Physiology · doi:10.1146/annurev-physiol-030212-183754 · Cited by 599 · see citing works
Self-citationVoltage‐gated and resting membrane currents recorded from B‐cells in intact mouse pancreatic islets
Göpel, S., Kanno, T., Barg, S., Galvanovskis, J., & Rorsman, P. (1999) · The Journal of Physiology · doi:10.1111/j.1469-7793.1999.00717.x · Cited by 226 · see citing works
Self-citationModel for synchronization of pancreatic beta-cells by gap junction coupling
Sherman, A., & Rinzel, J. (1991) · Biophysical Journal · doi:10.1016/s0006-3495(91)82271-8 · Cited by 181 · see citing works
Pérez-Armendariz, M., Roy, C., Spray, D. C., & Bennett, M. V. (1991) · Biophysical Journal · doi:10.1016/s0006-3495(91)82200-7 · Cited by 165 · see citing works
Why pancreatic islets burst but single beta cells do not. The heterogeneity hypothesis
Smolen, P., Rinzel, J., & Sherman, A. (1993) · Biophysical Journal · doi:10.1016/s0006-3495(93)81539-x · Cited by 158 · see citing works
Göpel, S. O., Kanno, T., Barg, S., Eliasson, L., Galvanovskis, J., Renström, E., & Rorsman, P. (1999) · The Journal of General Physiology · doi:10.1085/jgp.114.6.759 · Cited by 137 · see citing works
Self-citation
- Authors
- Smith, P. A., Ashcroft, F. M., & Rorsman, P.
- Affiliations
- University of Oxford (GB)
- Corresponding author
- Ashcroft, Frances M.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1990-02-12
- Volume
- 261
- Issue
- 1
- Pages
- 187-190
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2065249636
- Cited by
- 169
- References
- 0
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 1 · 2023: 2 · 2022: 1 · 2021: 4 · 2020: 3 · 2019: 1 · 2018: 1 · 2017: 1 · 2015: 1 · 2014: 7 · 2013: 5 · 2012: 4
- FWCI
- 13.75
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Keywords
- pancreatic beta cells, ATP-sensitive potassium channels, input conductance, membrane potential, slow waves, tolbutamide, primary tissue culture
- MeSH terms
- Adenosine Triphosphate, Animals, Cells, Cultured, Electric Conductivity, Glucose, Islets of Langerhans, Membrane Potentials, Tolbutamide, Potassium Channels, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
