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Cited by 226Pancreatic function and diabetesIon channel regulation and functionConnexins and lens biology

Voltage‐gated and resting membrane currents recorded from B‐cells in intact mouse pancreatic islets

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Göpel, S., Kanno, T., Barg, S., Galvanovskis, J., Rorsman, P.·1999·The Journal of Physiology
DOI 10.1111/j.1469-7793.1999.00717.xPMC PMC2269694
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Abstract

1. The perforated patch whole-cell configuration of the patch-clamp technique was applied to superficial cells in intact pancreatic islets. Immunostaining in combination with confocal microscopy revealed that the superficial cells consisted of 35 % insulin-secreting B-cells and 65 % non-B-cells (A- and D-cells). 2. Two types of cell, with distinct electrophysiological properties, could be functionally identified. One of these generated oscillatory electrical activity when the islet was exposed to 10 mM glucose and had the electrophysiological characteristics of isolated B-cells maintained in tissue culture. 3. The Ca2+ current recorded from B-cells in situ was 80 % larger than that of isolated B-cells. It exhibited significant (70 %) inactivation during 100 ms depolarisations. The inactivation was voltage dependent and particularly prominent during depolarisations evoking the largest Ca2+ currents. 4. Voltage-dependent K+ currents were observed during depolarisations to membrane potentials above -20 mV. These currents inactivated little during a 200 ms depolarisation and were unaffected by varying the holding potential between -90 and -30 mV. 5. The maximum resting conductance in the absence of glucose, which reflects the conductance of ATP-regulated K+ (KATP) channels, amounted to approximately 4 nS. Glucose produced a concentration-dependent reduction of KATP channel conductance with half-maximal inhibition observed with 5 mM glucose. 6. Combining voltage- and current-clamp…

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Crossref · 10.1111/j.1469-7793.1999.00717.x

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  • The reported glucose-activated CFTR current in mouse β-cells corresponds to a whole-cell conductance of 20 nS, which is too large: it would clamp the membrane potential at the Cl- equilibrium potential and prevent action potential firing.

    “The reported (244) current-voltage relationship of the glucose-activated CFTR current in metabolically intact mouse β-cells corresponds to a whole-cell conductance of 20 nS. This is 5-fold larger than the resting conductance recorded from metabolically intact β-cells in the absence of glucose (221), and 10-fold larger than the peak voltage-gated Ca2+ current (see legend to Figure 9) (221). With CF”

    From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [221]

  • Steady-state inactivation of the human β-cell Na+ current is half-maximal at -40 mV, approximately 60 mV more positive than the corresponding value in mouse β-cells.

    “Steady-state inactivation is half-maximal at -40 mV, ≈60 mV more positive than the corresponding value in mouse β-cells (221, 514) (Figure 10).”

    From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [221]

  • The mouse β-cell Na+ channel is highly unusual in the voltage dependence of its inactivation; in most β-cells it inactivates at such negative membrane potentials that little or no Na+ current remains within the physiological range.

    “The mouse (and rat) β-cell Na+ channel is highly unusual in the voltage dependence of its inactivation. In most β-cells, it inactivates at such negative membrane potentials that little or no Na+ current remains within the physiological range of membrane potentials (221, 514, 761) (Figure 10).”

    From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [221]

  • The membrane conductance of β-cells in intact islets at 20 mM glucose is about 1 nS, 10-fold higher than that observed in dispersed single β-cells.

    “The membrane conductance of β-cells in intact islets (measured in perforated patch recordings at 20 mM glucose as outlined in Figure 11A) is ~1 nS (221), 10-fold higher than that observed in dispersed single β-cells (631).”

    From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [221]

  • The background inward current must depolarize the membrane to ~-50 mV, which is the threshold for electrical activity and the membrane potential at which voltage-gated Ca2+ channels start to open.

    “The background inward current must depolarize the membrane to ~-50 mV, which is the threshold for electrical activity and the membrane potential at which voltage-gated Ca2+ channels start to open (221) (Figure 9).”

    From Pancreatic β-Cell Electrical Activity and Insulin Secretion: Of Mice and Men (2018) · cites this paper as [221]

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Authors
Göpel, S., Kanno, T., Barg, S., Galvanovskis, J., & Rorsman, P.
Affiliations
Lund University (SE)
ORCID
Venue
The Journal of Physiology
Publisher
Wiley
Published
1999-12
Volume
521
Issue
3
Pages
717-728
ISSN
  • 0022-3751 (ISSN-L)
  • 1469-7793
DOI
10.1111/j.1469-7793.1999.00717.x
PMC
PMC2269694
OpenAlex
W2017509811
Cited by
226
References
32
Citations by year
2026: 4 · 2025: 2 · 2024: 5 · 2023: 4 · 2022: 7 · 2021: 10 · 2020: 7 · 2019: 7 · 2018: 4 · 2017: 10 · 2016: 8 · 2015: 9 · 2014: 8 · 2013: 12 · 2012: 12
FWCI
5.27
Type
journal article
Language
en
Open access
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 1742 d)
Locations
  • The Journal of Physiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Lund University Publications (Lund University) (submittedVersion)
Topics
  • Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion channel regulation and function
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Connexins and lens biology
Keywords
pancreatic islets, B cells, voltage-gated calcium currents, voltage-dependent potassium current, ATP-sensitive potassium channels, electrical coupling, glucose stimulation, insulin secretion
MeSH terms
Algorithms, Animals, Cell Communication, Electrophysiology, Glucose, Islets of Langerhans, Membrane Potentials, Tetrodotoxin, Calcium Channels, Potassium Channels, Sodium Channels, Ion Channel Gating, Gap Junctions, Patch-Clamp Techniques, ATP-Binding Cassette Transporters, Microscopy, Confocal, Potassium Channels, Inwardly Rectifying, Mice, KATP Channels

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