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Purification of a Ca2+/calmodulin‐dependent nitric oxide synthase from porcine cerebellum: Cofactor‐role of tetrahydrobiopterin
Abstract
L‐Arginine‐derived nitric oxide acts as an inter‐ and intracellular signal molecule with cytosolic guanylyl cyclase as the effector system. Two NO synthase isoenzymes are postulated: a cytokine‐inducible enzyme in macrophages and a constitutive, Ca 2+‐regulated enzyme in various other cells. An NO synthase was isolated from porcine cerebellum by ammonium sulfate precipitation and affinity chromatography on 2',5'‐ADP‐Sepharose. The enzyme was identified as an NO synthase with a specific NO‐chemiluminescence method and with purified cytosolic guanylyl cyclase as an NO‐sensitive detection system. The purified NO synthase was, besides Ca 2+/calmodulin and NADPH, largely dependent on tetrahydrobiopterin as a cofactor.
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Cites
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Cited by
Nitric oxide as a secretory product of mammalian cells
Nathan, C. (1992) · The FASEB Journal · doi:10.1096/fasebj.6.12.1381691 · Cited by 4,350
Nitric oxide synthases in mammals
Knowles, R. G., & Moncada, S. (1994) · Biochemical Journal · doi:10.1042/bj2980249 · Cited by 2,752
Cloned and expressed nitric oxide synthase structurally resembles cytochrome P-450 reductase
Bredt, D. S., Hwang, P. M., Glatt, C. E., Lowenstein, C., Reed, R. R., & Snyder, S. H. (1991) · Nature · doi:10.1038/351714a0 · Cited by 2,354
Gases as biological messengers: nitric oxide and carbon monoxide in the brain
Dawson, T., & Snyder, S. (1994) · The Journal of Neuroscience · doi:10.1523/jneurosci.14-09-05147.1994 · Cited by 1,043
Mammalian nitric oxide synthases
Stuehr, D. J. (1999) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/s0005-2728(99)00016-x · Cited by 996
Metadata
- Authors
- Mayer, B., John, M., & Böhme, E.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1990-12-17
- Volume
- 277
- Issue
- 1-2
- Pages
- 215-219
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1943358986
- Cited by
- 449
- References
- 1
- FWCI
- 22.24
- Type
- journal article
- Language
- en
- Open access
- closed
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- Mayer, B., John, M., & Böhme, E. (1990). Purification of a Ca2+/calmodulin‐dependent nitric oxide synthase from porcine cerebellum: Cofactor‐role of tetrahydrobiopterin. FEBS Letters. https://doi.org/10.1016/0014-5793(90)80848-d
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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