Norepinephrine-induced synthesis of the uncoupling protein thermogenin (UCP) and its mitochondrial targeting in brown adipocytes differentiated in culture
Open accessAbstract
Synthesis of the brown adipocyte-specific mitochondrial uncoupling protein thermogenin (UCP) is demonstrated here in brown adipocytes differentiated in culture from precursor cells. By immunoblotting, no UCP was detectable in untreated multilocular adipocytes. The synthesis of UCP was stimulated by norepinephrine at physiological concentrations and was observable already after 2 h. It was evident from immunoelectron microscopy that the newly synthesised protein was targeted to the mitochondrial inner membrane, demonstrating the functional competence of these cultured cells.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
Catecholamines such as noradrenaline activate β3 adrenergic receptors and upregulate UCP-1 expression in mouse, rat, and human brown adipose tissues.
“These molecules are also known to upregulate UCP-1 expression in many models, including mouse [40,41], rat [42,43], and human [44] brown adipose tissues.”
From Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases (2019) · cites this paper as [43]
Cites
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
LAEMMLI, U. K. (1970) · Nature · doi:10.1038/227680a0 · Cited by 249,961 · see citing works
Silver staining of proteins in polyacrylamide gels
Wray, W., Boulikas, T., Wray, V. P., & Hancock, R. (1981) · Analytical Biochemistry · doi:10.1016/0003-2697(81)90179-2 · Cited by 3,481 · see citing works
Disk Electrophoresis of Basic Proteins and Peptides on Polyacrylamide Gels
Reisfeld, R., Lewis, U., & Williams, D. E. (1962) · Nature · doi:10.1038/195281a0 · Cited by 2,569 · see citing works
Kyhse‐Andersen, J. (1984) · Journal of Biochemical and Biophysical Methods · doi:10.1016/0165-022x(84)90040-x · Cited by 2,505 · see citing works
Isolation of the uncoupling protein from brown adipose tissue mitochondria
Lin, C. S., & Klingenberg, M. (1980) · FEBS Letters · doi:10.1016/0014-5793(80)80613-2 · Cited by 269 · see citing works
Circulation, Respiration, and Metabolism
(1985) · Proceedings in life sciences · doi:10.1007/978-3-642-70610-3 · Cited by 238 · see citing works
Exclusive occurrence of thermogenin antigen in brown adipose tissue
Cannon, B., Hedin, A., & Nedergaard, J. (1982) · FEBS Letters · doi:10.1016/0014-5793(82)81319-7 · Cited by 164 · see citing works
Self-citationDevelopment of brown fat cells in monolayer culture
Néchad, M., Kuusela, P., Carneheim, C., Björntorp, P. A., Nedergaard, J., & Cannon, B. (1983) · Experimental Cell Research · doi:10.1016/0014-4827(83)90384-1 · Cited by 154 · see citing works
Self-citationBouillaud, F., Ricquier, D., Mory, G., & Thibault, J. (1984) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)90902-6 · Cited by 142 · see citing works
Noradrenaline controls the concentration of the uncoupling protein in brown adipose tissue
Mory, G., Bouillaud, F., Combes‐George, M., & Ricquier, D. (1984) · FEBS Letters · doi:10.1016/0014-5793(84)80120-9 · Cited by 113 · see citing works
Epididymal white adipose tissue after cold stress in rats II. Mitochondrial changes
Lonćar, D., Afzelius, B. A., & Cannon, B. (1988) · Journal of Ultrastructure and Molecular Structure Research · doi:10.1016/0889-1605(88)90010-9 · Cited by 111 · see citing works
Self-citationRapid increase of mitochondrial uncoupling protein and its mRNA in stimulated brown adipose tissue
Ricquier, D., Mory, G., Bouillaud, F., Thibai, J., & Weissenbach, J. (1984) · FEBS Letters · doi:10.1016/0014-5793(84)80608-0 · Cited by 96 · see citing works
Rehnmark, S., Kopecký, J., Jacobsson, A., Néchad, M., Herron, D. K., Nelson, B. D., Obregón, M.-J., Nedergaard, J., & Cannon, B. (1989) · Experimental Cell Research · doi:10.1016/0014-4827(89)90280-2 · Cited by 76 · see citing works
Self-citationNoradrenergic stimulation of mitochondriogenesis in brown adipocytes differentiating in culture
Néchad, M., Nedergaard, J., & Cannon, B. (1987) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1987.253.6.c889 · Cited by 69 · see citing works
Self-citationα- and β-adrenergic control of thermogenin mRNA expression in brown adipose tissue
Jacobsson, A., Nedergaard, J., & Cannon, B. (1986) · Bioscience Reports · doi:10.1007/bf01114756 · Cited by 68 · see citing works
Self-citationTurner, B. M. (1983) · Journal of Immunological Methods · doi:10.1016/0022-1759(83)90204-1 · Cited by 67 · see citing works
Cinti, S., Zancanaro, C., Sbarbati, A., Cicolini, M., Vogel, P., Ricquier, D., & Fakan, S. (1989) · Biology of the Cell · doi:10.1111/j.1768-322x.1989.tb00883.x · Cited by 45 · see citing works
Physiological activation of brown adipose tissue destabilizes thermogenin mRNA
Jacobsson, A., Cannon, B., & Nedergaard, J. (1987) · FEBS Letters · doi:10.1016/0014-5793(87)80483-0 · Cited by 44 · see citing works
Self-citationExpression of the mitochondrial uncoupling protein in brown adipocytes
Forest, C. D., Doglio, A., Casteilla, L., Ricquier, D., & Ailhaud, G. P. (1987) · Experimental Cell Research · doi:10.1016/0014-4827(87)90431-9 · Cited by 35 · see citing works
Houštěk, J., Janíková, D., Bednář, J., Kopecký, J., Šebestián, J., & Soukup, T. (1990) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/0005-2728(90)90077-h · Cited by 30 · see citing works
Kuusela, P., Nedergaard, J., & Cannon, B. (1986) · Life Sciences · doi:10.1016/0024-3205(86)90052-4 · Cited by 29 · see citing works
Self-citationEffects of cholera toxin on gene expression in brown preadipocytes differentiating in culture
Herron, D. K., Néchad, M., Rehnmark, S., Nelson, B. D., Nedergaard, J., & Cannon, B. (1989) · American Journal of Physiology-Cell Physiology · doi:10.1152/ajpcell.1989.257.5.c920 · Cited by 24 · see citing works
Self-citationKing, T. P., & Lean, M. E. J. (1986) · Biochemical Society Transactions · doi:10.1042/bst0140289a · Cited by 7 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
Mitochondrial Uncoupling: A Key Controller of Biological Processes in Physiology and Diseases
Demine, S., Renard, P., & Arnould, T. (2019) · Cells · doi:10.3390/cells8080795 · Cited by 476 · see citing works
“These molecules are also known to upregulate UCP-1 expression in many models, including mouse [40,41], rat [42,43], and human [44] brown adipose tissues.”
BackgroundGolozoubova, V., Gullberg, H., Matthias, A., Cannon, B., Vennström, B., & Nedergaard, J. (2004) · Molecular Endocrinology · doi:10.1210/me.2003-0267 · Cited by 155 · see citing works
Self-citationSex Steroid Receptor Expression Profile in Brown Adipose Tissue. Effects of Hormonal Status
Rodriguez-Cuenca, S., Monjo, M., Frontera, M., Gianotti, M., Proenza, A. M., & Roca, P. (2007) · Cellular Physiology and Biochemistry · doi:10.1159/000110448 · Cited by 92 · see citing works
- Authors
- Herron, D., Rehnmark, S., Néchad, M., Lončar, D., Cannon, B., & Nedergaard, J.
- Affiliations
- Stockholm University (SE)
- Centre National de la Recherche Scientifique (FR)
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1990-07-30
- Volume
- 268
- Issue
- 1
- Pages
- 296-300
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2060474003
- Cited by
- 43
- References
- 23
- Citations by year
- 2023: 2 · 2021: 1 · 2019: 1 · 2018: 3 · 2015: 2 · 2013: 1
- FWCI
- 2.42
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Centre National de la Recherche Scientifique
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- thermogenin, uncoupling proteins, norepinephrine, brown adipocytes, mitochondrial targeting, inner mitochondrial membrane, protein synthesis, adipogenesis, thermogenesis
- MeSH terms
- Uncoupling Protein 1, Animals, Adipose Tissue, Brown, Carrier Proteins, Cell Compartmentation, Cell Differentiation, Cells, Cultured, Immunohistochemistry, Intracellular Membranes, Ion Channels, Male, Membrane Proteins, Microscopy, Electron, Mitochondria, Norepinephrine, Mitochondrial Proteins, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
