Phosphorylation of JAK2 in thrombin-stimulated human platelets
Open accessAbstract
We show the presence of the tyrosine kinase JAK2 in human platelets and demonstrate that it undergoes phosphorylation on tyrosine residues on challenge with the G protein receptor stimulus, thrombin, or the tyrosine phosphatase inhibitor, peroxovanadate. Thrombin-induced phosphorylation of JAK2 is inhibited by two structurally distinct inhibitors of tyrosine kinases, staurosporine and the tyrphostin ST271. The protein kinase C (PKC) inhibitor, Ro 31-8220, and intracellular Ca2+ chelator, BAPTA-AM, also inhibit thrombin-induced phosphorylation of JAK2, while the phorbol ester, phorbol dibutyrate (PDBu), and Ca2+ ionophore, A23187, induce tyrosine phosphorylation of JAK2. These results suggest that tyrosine phosphorylation of JAK2 stimulated by thrombin may be mediated downstream of phosphoinositide metabolism.
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JAK3 is constitutively active in human platelets.
“JAK3, which is constitutively active in human platelets”
From Targeting Janus Kinases and Signal Transducer and Activator of Transcription 3 To Treat Inflammation, Fibrosis, and Cancer: Rationale, Progress, and Caution (2020) · cites this paper as Rodriguez-Linares and Watson, 1994
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Bharadwaj, U., Kasembeli, M. M., Robinson, P., & Tweardy, D. J. (2020) · Pharmacological Reviews · doi:10.1124/pr.119.018440 · Cited by 314 · see citing works
“…et al., 1997; Ezumi et al., 1998; Laffargue et al., 1999; Pasquet et al., 2000), including JAK3, which is constitutively active in human platelets (Rodriguez-Linares and Watson, 1994; Witthuhn et al., 1999; Imada and Leonard, 2000), Thrombin treatment activates STAT1 and STAT3 (Tibbles et al.,…”
Background
- Authors
- Rodríguez-Liñares, B., & Watson, S. P.
- Affiliations
- University of Oxford (GB)
- Corresponding author
- Rodríguez-Liñares, Belén
- ORCID
- Watson, Steve P.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1994-10-03
- Volume
- 352
- Issue
- 3
- Pages
- 335-338
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2149188763
- Cited by
- 53
- References
- 0
- Citations by year
- 2025: 1 · 2021: 1 · 2020: 1 · 2014: 2 · 2013: 1 · 2012: 2
- FWCI
- 3.51
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Wellcome Trust
- Topics
- Health Sciences › Medicine › Hematology › Platelet Disorders and Treatments
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Keywords
- JAK2, tyrosine phosphorylation, thrombin, human platelets, tyrosine kinase, staurosporine, protein kinase C, phosphoinositide metabolism, intracellular calcium, peroxovanadate, phorbol dibutyrate, A23187
- MeSH terms
- Calcimycin, Alkaloids, Blood Platelets, Chelating Agents, Egtazic Acid, Indoles, Kinetics, Phosphorylation, Protein Kinase C, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Styrenes, Thrombin, Vanadates, Staurosporine, Janus Kinase 2, In Vitro Techniques
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