The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Characterization of a novel protein‐binding module — the WW domain
Abstract
We have identified, characterized and cloned human, mouse and chicken cDNA of a novel protein that binds to the Src homology domain 3 (SH3) of the Yes proto‐oncogene product. We subsequently named it YAP for Yes‐associated protein. Analysis of the YAP sequence revealed a protein module that was found in various structural, regulatory and signaling molecules. Because one of the prominent features of this sequence motif is the presence of two conserved tryptophans (W), we named it the WW domain. Using a functional screen of a cDNA expression library, we have identified two putative ligands of the WW domain of YAP which we named WBP‐1 and WBP‐2. Peptide sequence comparison between the two partial clones revealed a homologous proline‐rich region. Binding assays and site‐specific mutagenesis have shown that the proline‐rich motif binds with relatively high affinity and specificity to the WW domain of YAP, with a preliminary consensus that is different from the SH3‐binding PXXP motif. This suggests that the WW domain has a role in mediating protein‐protein interactions via proline‐rich regions, similar but distinct from Src homology 3 (SH3) domains. Based on this finding, we hypothesize that additional protein modules exist and that they could be isolated using proline‐rich peptides as functional probes.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Modular binding domains in signal transduction proteins
Cohen, G. B., Ren, R., & Baltimore, D. (1995) · Cell · doi:10.1016/0092-8674(95)90406-9 · Cited by 951
The WW domain: a signalling site in dystrophin?
Bork, P., & Sudol, M. (1994) · Trends in Biochemical Sciences · doi:10.1016/0968-0004(94)90053-1 · Cited by 388
Self-citationSudol, M., Bork, P., Einbond, A., Kastury, K., Druck, T., Negrini, M., Huebner, K., & Lehman, D. (1995) · Journal of Biological Chemistry · doi:10.1074/jbc.270.24.14733 · Cited by 342
Self-citation
Cited by
Stimulus-responsive hydrogels: theory, modern advances, and applications
Koetting, M. C., Peters, J. T., Steichen, S. D., & Peppas, N. A. (2015) · Materials Science and Engineering: R: Reports · doi:10.1016/j.mser.2015.04.001 · Cited by 1,191
β-Catenin-Driven Cancers Require a YAP1 Transcriptional Complex for Survival and Tumorigenesis
Rosenbluh, J., Nijhawan, D., Cox, A. G., Li, X., Neal, J. T., Schafer, E. J., Zack, T. I., Wang, X., Tsherniak, A., Schinzel, A. C., et al. (2012) · Cell · doi:10.1016/j.cell.2012.11.026 · Cited by 757
Ranganathan, R., Lu, K. P., Hunter, T., & Noel, J. P. (1997) · Cell · doi:10.1016/s0092-8674(00)80273-1 · Cited by 699
The Ubiquitin-Proteasome Pathway and Its Role in Cancer
Mani, A., & Gelmann, E. P. (2005) · Journal of Clinical Oncology · doi:10.1200/jco.2005.05.081 · Cited by 549
Borg, J.-P., Ooi, J., Levy, E., & Margolis, B. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.11.6229 · Cited by 477
Metadata
- Authors
- Sudol, M., Chen, H. I., Bougeret, C., Einbond, A., & Bork, P.
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1995-08
- Volume
- 369
- Issue
- 1
- Pages
- 67-71
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W2015338902
- Cited by
- 379
- References
- 3
- FWCI
- 7.08
- Type
- journal article
- Language
- en
- Open access
- closed
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Characterization of a novel protein‐binding module — the WW domain. https://app.cochat.ai/papers/10.1016/0014-5793(95)00550-s
- The paper
- Sudol, M., Chen, H. I., Bougeret, C., Einbond, A., & Bork, P. (1995). Characterization of a novel protein‐binding module — the WW domain. FEBS Letters. https://doi.org/10.1016/0014-5793(95)00550-s
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
