IL-10 induces DNA binding activity of three STAT proteins (Stat1, Stat3, and Stat5) and their distinct combinatorial assembly in the promoters of selected genes
Open accessAbstract
Interaction of IL-10 with its receptor leads to the activation of STAT transcription factors. Herein we report the IL-10 dependent simultaneous activation of three STAT transcription factors: Stat1, Stat3, and Stat5. Upon IL-10 treatment multiple Stat proteins become simultaneously activated, and bind to different promoters with equal kinetics but form distinct homo- and heterodimeric transcriptionally active complexes depending on the STAT-consensus elements of a selected gene promoter. Upon IL-10 treatment Stat1, 3, and 5 bind to the GRR of the FcgammaRI gene, activated Stat1 and 3 bind to the SIE sequence of the c-fos promoter and transcriptionally active Stat5 assembles at the PRL-STAT consensus sequence of the beta-casein gene. Thus, functionally relevant STAT dimerization is influenced by the activated cytokine receptor as well as the specific STAT consensus sequence present in a specific gene promoter.
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STAT1 has been reported to be activated by IL-10.
“Because STAT1 has been reported to be activated by IL-10 (24, 25), the specificity of the patterns of JAK-STAT signaling was evaluated by measuring the levels of STAT1 activation.”
From Cytokine Response Is Determined by Duration of Receptor and Signal Transducers and Activators of Transcription 3 (STAT3) Activation (2013) · cites this paper as [25]
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Research works printed but not linked: 2
- Silvennoinen, O., Witthuhn, B.A., Quelle, F.W., Cleveland, J.L., Yi, T. and Ihle, J.N. (1993) Proc. Natl. Acad. Sci. USA 90, 842% 8433.
- Ho, A.S.Y., Liu, Y., Khan, T.A., Hsu, D.-H., Bazan, J.F. and Moore, K.W. (1993) Proc. Natl. Acad. Sci. USA 90, 11267- 11271.
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Moore, K. W., de Waal Malefyt, R., Coffman, R. L., & O'Garra, A. (2001) · Annual Review of Immunology · doi:10.1146/annurev.immunol.19.1.683 · Cited by 6,737 · see citing works
Braun, D. A., Fribourg, M., & Sealfon, S. C. (2013) · Journal of Biological Chemistry · doi:10.1074/jbc.m112.386573 · Cited by 168 · see citing works
“Because STAT1 has been reported to be activated by IL-10 (24, 25), the specificity of the patterns of JAK-STAT signaling was evaluated by measuring the levels of STAT1 activation.”
BackgroundJAK-STAT in Lipid Metabolism of Adipocytes
Xu, D., Yin, C., Wang, S., & Xiao, Y. (2013) · JAK-STAT · doi:10.4161/jkst.27203 · Cited by 69 · see citing works
Reprogramming macrophage orientation by microRNA 146b targeting transcription factor IRF5
Peng, L., Zhang, H., Hao, Y., Xu, F., Yang, J., Zhang, R., Lu, G., Zheng, Z., Cui, M., Qi, C.-F., et al. (2016) · EBioMedicine · doi:10.1016/j.ebiom.2016.10.041 · Cited by 67 · see citing works
Kang, H.-J., Seo, H.-A., Go, Y., Oh, C. J., Jeoung, N. H., Park, K.-G., & Lee, I.-K. (2013) · PLoS ONE · doi:10.1371/journal.pone.0061411 · Cited by 49 · see citing works
“Because activation of STAT3 is induced by cytokines such as IL-6 and IL-10 [20,21], DMF may have the potential to function as a STAT3 inhibitor.”
Background
- Authors
- Wehinger, J., Gouilleux, F., Groner, B., Finke, J., Mertelsmann, R., & Maria Weber-Nordt, R.
- Affiliations
- University of Freiburg (DE)
- Inserm (FR)
- Venue
- FEBS Letters
- Publisher
- Wiley
- Published
- 1996-10-07
- Volume
- 394
- Issue
- 3
- Pages
- 365-370
- ISSN
- 0014-5793 (ISSN-L)
- 1873-3468
- OpenAlex
- W1973155132
- Cited by
- 167
- References
- 0
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 6 · 2021: 4 · 2020: 4 · 2019: 6 · 2018: 3 · 2017: 7 · 2016: 11 · 2015: 2 · 2014: 7 · 2013: 12 · 2012: 6
- FWCI
- 2.40
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- FEBS Letters (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- FreiDok plus (Universitätsbibliothek Freiburg) (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft
- Topics
- Health Sciences › Medicine › Oncology › Cytokine Signaling Pathways and Interactions
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Receptor Mechanisms and Signaling
- Keywords
- IL-10, STAT1, STAT3, STAT5, transcription factor activation, cytokine receptor signaling, promoter binding, Fc gamma receptor I, c-fos promoter, beta-casein promoter
- MeSH terms
- B-Lymphocytes, Caseins, Cells, Cultured, DNA, DNA-Binding Proteins, Gene Expression Regulation, Hematopoietic Stem Cells, Milk Proteins, Promoter Regions, Genetic, Protein Binding, Trans-Activators, Interleukin-10, Genes, fos, Receptors, IgG, STAT1 Transcription Factor, STAT3 Transcription Factor, STAT5 Transcription Factor
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