Cited by 241Glycosylation and Glycoproteins ResearchProteoglycans and glycosaminoglycans researchDigestive system and related health

Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin

Tytgat, Kristien M.A.J., Büller, Hans A., Opdam, Frank J.M., Kim, Young S., Einerhand, Alexandra W.C., Dekker, Jan·1994·Gastroenterology

Abstract

Background/aims Human colonic epithelium produces large amounts of mucin. The aim of this study was to examine mucin biosynthesis in the human colon.Methods Human colonic mucin was isolated using CsCl density gradients, and polyclonal antiserum was raised. Biosynthesis of colonic mucins was studied by labeling colonic explants with 35S-labeled amino acids or [35S]sulfate and subsequent immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).Results The polyclonal antiserum specifically recognized colonic mucin, primarily reacting with peptide epitopes. Biosynthetic pulse/chase experiments showed a 35S-amino acid-labeled mucin precursor of about 600 kilodaltons, which was converted into a mature, glycosylated, and sulfated mucin and subsequently secreted into the medium. This mature mucin comigrated with isolated colonic mucin with an apparent molecular weight of 550 kilodaltons on SDS-PAGE, whereas gel filtration indicated that the molecular weight is actually much larger. Independent immunoprecipitation with an anti-Muc2 antiserum showed cross-reactivity with the 600-kilodalton precursor.Conclusions These results show the biosynthesis of a secretory colonic mucin for the first time. This mucin is synthesized as a precursor protein of approximately 600 kilodaltons, which, after glycosylation, is secreted as a glycoprotein with an apparent molecular weight of 550 kilodaltons on SDS-PAGE. It is very likely that this mucin is Muc2.

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Crossref · 10.1016/0016-5085(94)90537-1

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Authors
Tytgat, K. M. A. J., Büller, H. A., Opdam, F. J. M., Kim, Y. S., Einerhand, A. W. C., & Dekker, J.
Affiliations
  • Amsterdam UMC Location University of Amsterdam (NL)
  • San Francisco VA Medical Center (US)
Venue
Gastroenterology
Publisher
Elsevier BV
Published
1994-11
Volume
107
Issue
5
Pages
1352-1363
ISSN
0016-5085 (ISSN-L)
OpenAlex
W1963832148
Cited by
241
References
53
Citations by year
2026: 1 · 2025: 1 · 2024: 5 · 2023: 2 · 2022: 9 · 2021: 10 · 2020: 9 · 2019: 7 · 2018: 4 · 2017: 2 · 2016: 5 · 2015: 5 · 2014: 6 · 2013: 5 · 2012: 4
FWCI
3.14
Type
journal article
Language
en
Open access
closed
Locations
  • Gastroenterology (publishedVersion)
  • PubMed (publishedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion)
  • Pure Amsterdam UMC (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Digestive system and related health
Keywords
colonic mucin, MUC2, mucin production, secretory mucins, colonic epithelium, glycosylation, sulfation, mucin secretion
MeSH terms
Colon, Electrophoresis, Polyacrylamide Gel, Glycosylation, Humans, Immune Sera, Intestinal Mucosa, Molecular Weight, Mucins, Precipitin Tests, Protein Precursors, Mucin-2

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