Biosynthesis of human colonic mucin: Muc2 is the prominent secretory mucin
Abstract
Background/aims Human colonic epithelium produces large amounts of mucin. The aim of this study was to examine mucin biosynthesis in the human colon.Methods Human colonic mucin was isolated using CsCl density gradients, and polyclonal antiserum was raised. Biosynthesis of colonic mucins was studied by labeling colonic explants with 35S-labeled amino acids or [35S]sulfate and subsequent immunoprecipitation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).Results The polyclonal antiserum specifically recognized colonic mucin, primarily reacting with peptide epitopes. Biosynthetic pulse/chase experiments showed a 35S-amino acid-labeled mucin precursor of about 600 kilodaltons, which was converted into a mature, glycosylated, and sulfated mucin and subsequently secreted into the medium. This mature mucin comigrated with isolated colonic mucin with an apparent molecular weight of 550 kilodaltons on SDS-PAGE, whereas gel filtration indicated that the molecular weight is actually much larger. Independent immunoprecipitation with an anti-Muc2 antiserum showed cross-reactivity with the 600-kilodalton precursor.Conclusions These results show the biosynthesis of a secretory colonic mucin for the first time. This mucin is synthesized as a precursor protein of approximately 600 kilodaltons, which, after glycosylation, is secreted as a glycoprotein with an apparent molecular weight of 550 kilodaltons on SDS-PAGE. It is very likely that this mucin is Muc2.
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Mucins constitute the major component of the mucus layer, and in the colon, Muc2 is the most prominent mucin expressed and secreted by goblet cells.
“Mucins constitute the major component of this mucus layer12 and in the colon, Muc2 is the most prominent mucin expressed and secreted by goblet cells.13”
From Using Corticosteroids to Reshape the Gut Microbiome: Implications for Inflammatory Bowel Diseases (2015) · cites this paper as [13]
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- Authors
- Tytgat, K. M. A. J., Büller, H. A., Opdam, F. J. M., Kim, Y. S., Einerhand, A. W. C., & Dekker, J.
- Affiliations
- Amsterdam UMC Location University of Amsterdam (NL)
- San Francisco VA Medical Center (US)
- Venue
- Gastroenterology
- Publisher
- Elsevier BV
- Published
- 1994-11
- Volume
- 107
- Issue
- 5
- Pages
- 1352-1363
- ISSN
- 0016-5085 (ISSN-L)
- OpenAlex
- W1963832148
- Cited by
- 241
- References
- 53
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 5 · 2023: 2 · 2022: 9 · 2021: 10 · 2020: 9 · 2019: 7 · 2018: 4 · 2017: 2 · 2016: 5 · 2015: 5 · 2014: 6 · 2013: 5 · 2012: 4
- FWCI
- 3.14
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Gastroenterology (publishedVersion)
- PubMed (publishedVersion)
- Data Archiving and Networked Services (DANS) (submittedVersion)
- Pure Amsterdam UMC (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Glycosylation and Glycoproteins Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Digestive system and related health
- Keywords
- colonic mucin, MUC2, mucin production, secretory mucins, colonic epithelium, glycosylation, sulfation, mucin secretion
- MeSH terms
- Colon, Electrophoresis, Polyacrylamide Gel, Glycosylation, Humans, Immune Sera, Intestinal Mucosa, Molecular Weight, Mucins, Precipitin Tests, Protein Precursors, Mucin-2
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