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Cited by 40Systemic Lupus Erythematosus ResearchSystemic Sclerosis and Related DiseasesMonoclonal and Polyclonal Antibodies Research

The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.

Dissociation studies of DNA/anti-DNA complexes in relation to anti-DNA avidity

Smeenk, Ruud J.T., Van Rooijen, Anita, Swaak, Tom J.G.·1988·Journal of Immunological Methods
DOI 10.1016/0022-1759(88)90438-3
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Abstract

Antibodies to dsDNA differ in their avidity towards the antigen. The electrostatic interaction between DNA and anti-DNA is sensitive to increases in pH and/or ionic strength and therefore, elution studies employing either of these permit discrimination between anti-dsDNA populations that differ in avidity. Another way to determine anti-dsDNA avidity is the calculation of Farr/PEG ratios. These are obtained by division of the amount of anti-DNA measured with the Farr assay (which does not detect low avidity anti-dsDNA) by the amount measured with the PEG assay (which does detect low avidity anti-dsDNA). With these separate approaches, we compared the sera of 17 SLE patients with nephritis with the sera of 17 patients with central nervous system (CNS) involvement. Farr/PEG ratios and sensitivity to high pH elution of anti-dsDNA in the sera of these patients both permitted discrimination between the two groups of patients. The anti-dsDNA of patients with nephritis was found to have a significantly higher avidity towards DNA than anti-dsDNA of patients with cerebral disease. We also observed a significant correlation between Farr/PEG ratios and the salt lability of anti-dsDNA.

Overview

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Crossref · 10.1016/0022-1759(88)90438-3

Cites

31 of 31 references verified0 citation errors · 0 not checkable

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Claims other papers make about this one

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  • Anti-dsDNA tests detect different subpopulations of anti-dsDNA with distinct fine specificities.

    “Many test methods are available and anti-dsDNA tests, by design, detect different subpopulations of anti-dsDNA with distinct fine specificities (16,20,(22)(23)(24).”

    From Anti-dsDNA Testing Specificity for Systemic Lupus Erythematosus: A Systematic Review. (2022) · cites this paper as [24]

Citations

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Research works printed but not linked: 1

  1. Koffler, 1974, Polynucleotide immune complexes in serum and glomeruli of patients with SLE, Am. J. Pathol., 74, 109

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Metadata

Authors
Smeenk, R. J. T., Van Rooijen, A., & Swaak, T. J. G.
Affiliations
  • University of Amsterdam (NL)
  • Daniel den Hoed Kliniek (NL)
Corresponding author
Smeenk, Ruud J.T.
Venue
Journal of Immunological Methods
Publisher
Elsevier BV
Published
1988-04
Volume
109
Issue
1
Pages
27-35
ISSN
0022-1759 (ISSN-L)
DOI
10.1016/0022-1759(88)90438-3
OpenAlex
W2078179857
Cited by
40
References
31
Citations by year
2026: 1 · 2025: 1 · 2023: 1 · 2021: 1 · 2020: 1 · 2018: 1 · 2017: 1
FWCI
4.31
Type
journal article
Language
en
Open access
closed
Licence
www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations
  • Journal of Immunological Methods (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Rheumatology › Systemic Lupus Erythematosus Research
  • Health Sciences › Medicine › Rheumatology › Systemic Sclerosis and Related Diseases
  • Life Sciences › Immunology and Microbiology › Immunology › Monoclonal and Polyclonal Antibodies Research
Keywords
anti-dsDNA antibodies, antibody avidity, systemic lupus erythematosus, lupus nephritis, central nervous system involvement, Farr assay, ionic strength, electrostatic interactions
MeSH terms
Antibody Affinity, Antigen-Antibody Complex, Antibodies, Antinuclear, DNA, Fluorescent Antibody Technique, Humans, Immunologic Techniques, Lupus Erythematosus, Systemic, Polyethylene Glycols

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