Decrease in oligodendrocyte carbonic anhydrase activity preceding myelin degeneration in cuprizone induced demyelination
Abstract
Both immunohistochemical and biochemical evidence is presented to show for the first time that carbonic anhydrase II (CA II) activity falls in the brain of mice in cuprizone (bis(cyclohexanone)oxalyldihydrazone) induced demyelination well before demyelination develops. This fall began during the first week, whereas the first signs of myelin degeneration induced by cuprizone did not appear until 3 weeks and demyelination in the superior cerebellar peduncle in the mouse took 6-8 weeks to develop. The findings suggest that oligodendrocyte CA II activity is essential either for the survival of oligodendrocytes or for the maintenance of central myelin.
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Many ICI and Swiss Webster mice lived for months on the cuprizone diet, though not all tolerated it.
“Though not tolerated by all of these mice, many of them mice lived for months on the cuprizone diet.”
From SJL Mice Exposed to Cuprizone Intoxication Reveal Strain and Gender Pattern Differences in Demyelination (2009) · cites this paper as [27]
Studies of ICI and Swiss Webster mice used 0.5% to 0.6% cuprizone in their diet to induce demyelination.
“Studies of ICI and Swiss Webster mice used 0.5% to 0.6% cuprizone in their diet to induce demyelination 8, 27, 31.”
From SJL Mice Exposed to Cuprizone Intoxication Reveal Strain and Gender Pattern Differences in Demyelination (2009) · cites this paper as [27]
Mitochondrial disturbances seem to be the key factor of oligodendroglial apoptosis.
“However, mitochondrial disturbances seem to be the key factor of oligodendroglial apoptosis, since enlarged “giant” mitochondria were detected in the liver and brain of cuprizone-treated mice (Suzuki, 1969; Hemm et al., 1971; Komoly et al., 1987).”
From Glial response during cuprizone-induced de- and remyelination in the CNS: lessons learned (2014) · cites this paper as Komoly et al., 1987
Cites
Blakemore, W. F. (1972) · Journal of Neurocytology · doi:10.1007/bf01102943 · Cited by 217 · see citing works
Demyelination of the superior cerebellar peduncle in the mouse induced by cuprizone
Blakemore, W. F. (1973) · Journal of the Neurological Sciences · doi:10.1016/0022-510x(73)90118-4 · Cited by 207 · see citing works
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Matsushima, G. K., & Morell, P. (2001) · Brain Pathology · doi:10.1111/j.1750-3639.2001.tb00385.x · Cited by 984 · see citing works
Microglial/macrophage accumulation during cuprizone-induced demyelination in C57BL/6 mice
Hiremath, M. M., Saito, Y., Knapp, G. W., Ting, J. P.-Y., Suzuki, K., & Matsushima, G. K. (1998) · Journal of Neuroimmunology · doi:10.1016/s0165-5728(98)00168-4 · Cited by 412 · see citing works
Glial response during cuprizone-induced de- and remyelination in the CNS: lessons learned
Gudi, V., Gingele, S., Skripuletz, T., & Stangel, M. (2014) · Frontiers in Cellular Neuroscience · doi:10.3389/fncel.2014.00073 · Cited by 375 · see citing works
“The activity of CA II started to decline already during the first week of cuprizone diet well before demyelination develops and (Komoly et al., 1987) persists until demyelination is complete.”
BackgroundInfluentialCuprizone [Bis(Cyclohexylidenehydrazide)] is Selectively Toxic for Mature Oligodendrocytes
Bénardais, K., Kotsiari, A., Škuljec, J., Koutsoudaki, P. N., Gudi, V., Singh, V., Vulinović, F., Skripuletz, T., & Stangel, M. (2013) · Neurotoxicity Research · doi:10.1007/s12640-013-9380-9 · Cited by 148 · see citing works
Taylor, L. C., Gilmore, W., & Matsushima, G. K. (2009) · Brain Pathology · doi:10.1111/j.1750-3639.2008.00230.x · Cited by 84 · see citing works
Sen, M. K., Almuslehi, M. S. M., Gyengesi, E., Myers, S. J., Shortland, P. J., Mahns, D. A., & Coorssen, J. R. (2019) · Cells · doi:10.3390/cells8111314 · Cited by 35 · see citing works
Cuprizone Administration Alters the Iron Metabolism in the Mouse Model of Multiple Sclerosis
Varga, E., Pandur, E., Abrahám, H., Horváth, A., Ács, P., Komoly, S., Miseta, A., & Sipos, K. (2018) · Cellular and Molecular Neurobiology · doi:10.1007/s10571-018-0578-5 · Cited by 27 · see citing works
Self-citation
- Authors
- Komoly, S., Jeyasingham, M. D., Pratt, O. E., & Lantos, P. L.
- Venue
- Journal of the Neurological Sciences
- Publisher
- Elsevier BV
- Published
- 1987-06
- Volume
- 79
- Issue
- 1-2
- Pages
- 141-148
- ISSN
- 0022-510X (ISSN-L)
- OpenAlex
- W2062959517
- Cited by
- 73
- References
- 2
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 1 · 2021: 3 · 2020: 3 · 2019: 4 · 2018: 1 · 2017: 4 · 2016: 4 · 2015: 4 · 2014: 2 · 2013: 10 · 2012: 3
- FWCI
- 0.41
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of the Neurological Sciences (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Enzyme function and inhibition
- Health Sciences › Medicine › Pathology and Forensic Medicine › Multiple Sclerosis Research Studies
- Life Sciences › Neuroscience › Neurology › Neurological diseases and metabolism
- Keywords
- carbonic anhydrase II, oligodendrocytes, cuprizone, demyelination, myelin degeneration, superior cerebellar peduncle, oligodendrocyte survival, myelin maintenance
- MeSH terms
- Animals, Brain, Carbonic Anhydrases, Cuprizone, Demyelinating Diseases, Myelin Basic Protein, Glial Fibrillary Acidic Protein, Male, Myelin Sheath, Nerve Degeneration, Neuroglia, Oligodendroglia, Time Factors, Mice
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