Exploration of the human fetal pituitary adrenal axis: stimulation of cortisol and dehydroepiandrosterone sulfate biosynthesis by homologous pituitary in organ culture
Abstract
Adrenals obtained from human abortices at midpregnancy were kept under conditions of tissue culture and the production of cortisol and dehydroepiandrosterone sulfate (3beta-hydroxy-5-androsten-17-one, 3-sulfate; DHA-S) monitored by radioimmunoassays for up to 2 weeks. Basal production of dehydroepiandrosterone sulfate was considerably higher than that of cortisol. alpha1-24corticotrophin, alpha1-39corticotrophin, (a long acting porcine corticotrophin) at the concentration of 1 mU/ml of culture medium in both instances, and dibutyryl cyclic AMP (1mM) enhanced the production of both steroids some 3 to 30 times above control values. Medium harvested from homologous pituitary cultures had comparable corticotrophic activity. It is concluded that at midpregnancy the regulation of corticoidogenesis implies the existence of a corticotrophic factor either identical or closely related to ACTH.
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ACTH stimulated the secretion of all measured metabolites in ex vivo cultured human fetal adrenal tissue.
“ACTH stimulated the secretion of all measured metabolites, which is in accordance with previous studies showing a stimulating effect on both cortisol, DHEAS, androstenedione and testosterone (9,30,31,39).”
From Establishment of a novel human fetal adrenal culture model that supports de novo and manipulated steroidogenesis (2021) · cites this paper as [30]
The continued production of androgens and glucocorticoids in this study contrasts with previous long-term human fetal adrenal tissue cultures, which showed a rapid time-dependent decrease in the production of both DHEAS and cortisol.
“The continued production of androgens and glucocorticoids reported in this study contrasts with previous long-term HFA tissue cultures which showed a rapid time-dependent decrease in the production of both DHEAS and cortisol (30).”
From Establishment of a novel human fetal adrenal culture model that supports de novo and manipulated steroidogenesis (2021) · cites this paper as [30]
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Branchaud, C. T., Goodyer, C. G., Hall, C. S. G., Arato, J. S., SiliBan, R. E., & Giroud, C. J. P. (1978) · Steroids · doi:10.1016/0039-128x(78)90037-5 · Cited by 53 · see citing works
Self-citationMelau, C., Nielsen, J. E., Perlman, S., Lundvall, L., Langhoff Thuesen, L., Juul Hare, K., Schou Hammerum, M., Frederiksen, H., Mitchell, R. T., Juul, A., et al. (2021) · The Journal of Clinical Endocrinology & Metabolism · doi:10.1210/clinem/dgaa852 · Cited by 16 · see citing works
- Authors
- Goodyer, C. G., Hall, C. S. G., Branchaud, C., & Giroud, C. J. P.
- Affiliations
- Montreal Children's Hospital (CA)
- ORCID
- Goodyer, C.G.
- Venue
- Steroids
- Publisher
- Elsevier BV
- Published
- 1977-03
- Volume
- 29
- Issue
- 3
- Pages
- 407-416
- ISSN
- 0039-128X (ISSN-L)
- OpenAlex
- W2092254274
- Cited by
- 11
- References
- 11
- Citations by year
- 2020: 1
- FWCI
- 0.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Steroids (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Endocrinology, Diabetes and Metabolism › Adrenal Hormones and Disorders
- Life Sciences › Neuroscience › Behavioral Neuroscience › Stress Responses and Cortisol
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Keywords
- cortisol production, dehydroepiandrosterone sulfate, ACTH, cyclic AMP, second trimester, adrenal steroidogenesis
- MeSH terms
- Dehydroepiandrosterone, Female, Fetus, Gestational Age, Humans, Hydrocortisone, Kinetics, Organ Culture Techniques, Pituitary Gland, Pituitary-Adrenal System, Pregnancy
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