Separation of estrogen conjugates by high pressure liquid chromatography
Abstract
High pressure liquid chromatography using a prepacked commercial strong anion exchanger column (mu Partisil 10 SAX, 25 cm x 4.6 mm) was used to separate a mixture of eight estrogen conjugates. Chromatographic conditions using a 0.01 M potassium phosphate or 0.1 M NaCl as solvent in the isocratic mode are described for the separation of estrone glucosiduronate, 17beta-estradiol-3-glucosiduronate, 17beta-estradiol-17-glucosiduronate, estriol-3-glucosiduronate, estriol-16alpha-glucosiduronate, estriol-17-glucosiduronate, estrone sulfate and 17beta-estradiol-3-sulfate. This system gives high resolution of the estrogen conjugates in small eluent volumes in less than 30 min. The advantages of this high pressure liquid chromatographic system over other methods of separation are discussed.
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Estrogens and catecholestrogens are efficiently sulfated at several positions.
“Estrogens and catecholestrogens are efficiently sulfated at several positions [[325], [326], [327]].”
From Human steroid biosynthesis, metabolism and excretion are differentially reflected by serum and urine steroid metabolomes: a comprehensive review (2019) · cites this paper as [326]
Cites
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Schiffer, L., Barnard, L., Baranowski, E. S., Gilligan, L. C., Taylor, A. E., Arlt, W., Shackleton, C. H. L., & Storbeck, K.-H. (2019) · The Journal of Steroid Biochemistry and Molecular Biology · doi:10.1016/j.jsbmb.2019.105439 · Cited by 450 · see citing works
- Authors
- Musey, P. I., Collins, D. C., & Preedy, J. R. K.
- Affiliations
- Emory University (US)
- Corresponding author
- Musey, P.I.
- Venue
- Steroids
- Publisher
- Elsevier BV
- Published
- 1978-04
- Volume
- 31
- Issue
- 4
- Pages
- 583-592
- ISSN
- 0039-128X (ISSN-L)
- OpenAlex
- W2037156118
- Cited by
- 29
- References
- 16
- Citations by year
- 2019: 1
- FWCI
- 2.81
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Steroids (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Analytical Chemistry and Chromatography
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Estrogen and related hormone effects
- Physical Sciences › Chemistry › Analytical Chemistry › Analytical Methods in Pharmaceuticals
- Keywords
- estrogen conjugates, glucuronide conjugates, estrone sulfate, estradiol sulfate, anion exchange chromatography, isocratic elution
- MeSH terms
- Chromatography, High Pressure Liquid, Estrogens, Conjugated (USP), Glucuronates, Humans, Sulfuric Acids
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