[26] Expression of recombinant vitamin K-dependent proteins in mammalian cells: Factors IX and VII
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Expressing rFVIIIFc and rFIXFc in HEK293 resulted in greater tyrosine sulfation and glutamic acid γ-carboxylation compared to CHO cells and excluded α-gal and Neu5Gc from the manufactured products.
“Expressing these glycoproteins in HEK293 resulted in greater tyrosine sulfation and glutamic acid γ-carboxylation compared to CHO cells and excluded any α-gal and Neu5Gc from the manufactured products (Berkner, 1993;Kannicht et al., 2013;Peters et al., 2013;McCue et al., 2014McCue et al., , 2015)).”
From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Berkner, 1993
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Therapeutic glycoprotein production in mammalian cells
Lalonde, M.-E., & Durocher, Y. (2017) · Journal of Biotechnology · doi:10.1016/j.jbiotec.2017.04.028 · Cited by 296 · see citing works
- Authors
- Berkner, K. L.
- Affiliations
- Cleveland Clinic (US)
- Corresponding author
- Berkner, Kathleen L.
- ORCID
- Berkner, Kathleen L.
- Venue
- Methods in Enzymology
- Publisher
- Elsevier
- Published
- 1993
- Volume
- 222
- Pages
- 450-477
- ISSN
- 0076-6879 (ISSN-L)
- OpenAlex
- W1598924174
- Cited by
- 74
- References
- 0
- Citations by year
- 2026: 3 · 2025: 1 · 2024: 1 · 2023: 1 · 2022: 3 · 2021: 1 · 2020: 2 · 2019: 5 · 2018: 1 · 2017: 4 · 2016: 3 · 2015: 2 · 2014: 2 · 2013: 4 · 2012: 3
- FWCI
- 0.49
- Type
- book chapter
- Language
- en
- Open access
- closed
- Locations
- Methods in enzymology on CD-ROM/Methods in enzymology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Health Sciences › Medicine › Hematology › Hemophilia Treatment and Research
- Health Sciences › Medicine › Hematology › Blood Coagulation and Thrombosis Mechanisms
- Keywords
- vitamin K-dependent proteins, factor IX, factor VII, recombinant protein expression, mammalian cell expression systems, cell line selection, protein carboxylation, protein secretion, protein C, coagulation factors
- MeSH terms
- Animals, Cell Line, Chromatography, High Pressure Liquid, Electrophoresis, Polyacrylamide Gel, Factor IX, Factor VII, Genetic Vectors, Carcinoma, Hepatocellular, Humans, Indicators and Reagents, Liver Neoplasms, Molecular Weight, Protein C, Protein Precursors, Protein Processing, Post-Translational, Recombinant Proteins, Transfection, Tumor Cells, Cultured, Vitamin K, Gene Expression
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