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Cited by 57Drug-Induced Adverse ReactionsT-cell and B-cell ImmunologyContact Dermatitis and Allergies

T cell reactivity to penicillin: phenotypic analysis of in vitro activated cell subsets

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Koponen, Marianne, Pichler, Werner J., de Weck, Alain L.·1986·Journal of Allergy and Clinical Immunology
DOI 10.1016/0091-6749(86)90083-7
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Abstract

Patients with penicillin allergy demonstrate a T cell proliferative response after in vitro stimulation with penicillin G (Pen G) and other beta-lactam antibiotics. To understand better penicillin-allergic reactions, T cell subset stimulation with Pen G was studied and compared with other soluble (tetanus toxoid and purified protein derivative [PPD]) and membrane-bound viral (influenza A and Epstein-Barr viruses) antigens. A double fluorescence method for flow cytometry was used to evaluate the activated cells simultaneously by pyronin Y staining of RNA and by indirect immunofluorescence of cell surface T4, T8, or Leu 8 antigens. The antigens used stimulated mainly the T4+ subset (greater than 90%), whereas the number of activated T8 cells was slightly increased only in Pen G- and influenza A-triggered cultures (5% to 15%). Leu 8 antigen was used to analyze more precisely the activated T4+ cells. Pen G and influenza A and Epstein-Barr viruses stimulated both T4+, Leu 8+ (greater than 50% of activated cells, inducers for suppressor cells), and T4+, Leu 8- (helpers for B cells) subsets, whereas PPD activated mainly T4+, Leu 8- subpopulations. These results indicate that penicillin-allergic patients with skin symptoms demonstrate a T cell subset stimulation that resembles more the reaction versus viral antigens (membrane incorporated) than to soluble antigens like PPD. These results suggest that Pen G is presented to T cells like viral proteins and might thus cause allergic reactions resembling skin symptoms observed in viral diseases.

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  • Elevated levels of IL-6 and IL-8 may indicate infection as the causative agent of AIN.

    “In a similar context, elevated levels of IL-6 and IL-8 may indicate infection as the causative agent of AIN [59, 98–101].”

    From The diagnosis of acute interstitial nephritis caused by infection versus antibiotic-induced interstitial nephritis: a narrative review (2024) · cites this paper as [101]

    The Discussion and Expression of Leu 8 antigen sections (page numbers unavailable) discuss T-cell responses to penicillin and viral antigens, not biomarkers for infection-caused AIN. Searches for IL-6, IL-8, and acute interstitial nephritis found no passage addressing the claim. (LLM, conf=0.76)

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Research works printed but not linked: 2

  1. [4]4. Walker C, Pichler WJ, de Week AL: Different T cell subset stimu ation by IgGl or IgG2a anti-T3 antibodies. Immuno- biology (in press)
  2. [9]9. Walker C, Pichler WJ, Koponen M, Domzig W, de Week AL: Different effects of IL-2 addition on antibody cross-linking on T cell subset stimulation by D3 antibodies. Clin Immunol (in press)

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Related

Authors
Koponen, M., Pichler, W. J., & de Weck, A. L.
Affiliations
University Hospital of Bern (CH)
ORCID
Pichler, Werner J.
Venue
Journal of Allergy and Clinical Immunology
Publisher
Elsevier BV
Published
1986-10
Volume
78
Issue
4
Pages
645-652
ISSN
0091-6749 (ISSN-L)
DOI
10.1016/0091-6749(86)90083-7
OpenAlex
W2062751853
Cited by
57
References
23
Citations by year
2024: 2 · 2020: 2 · 2014: 3
FWCI
2.27
Type
journal article
Language
en
Open access
Licence
Locations
  • Journal of Allergy and Clinical Immunology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Pharmacology › Drug-Induced Adverse Reactions
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
  • Health Sciences › Medicine › Dermatology › Contact Dermatitis and Allergies
Keywords
penicillin allergy, penicillin G, T cell subsets, T8 cells, regulatory T cells, T helper cells, beta-lactam antibiotics, influenza A virus, Epstein-Barr virus, tetanus toxoid, purified protein derivative, T cell activation, skin allergy, antigen presentation
MeSH terms
Anti-Bacterial Agents, Antigens, Differentiation, T-Lymphocyte, Antigens, Surface, Herpesvirus 4, Human, Humans, Lymphocyte Activation, Influenza A virus, Penicillin G, Phenotype, T-Lymphocytes, Tetanus Toxoid, Tuberculin

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