Cited by 58Bacterial Genetics and BiotechnologyChromosomal and Genetic VariationsAntibiotic Resistance in Bacteria

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The transposon Tn9 generates a 9 bp repeated sequence during integration

Johnsrud, Lorraine, Calos, Michèle P., Miller, Jeffrey H.·1978·Cell

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Abstract

We performed a genetic and sequencing analysis of insertions of the transposon Tn9 into the lac operon of E. coli. Genetic mapping of 70 insertions into lacl and Z shows that starting from the same point on the chromosome, Tn9 goes to at least 50 different points in these two genes. Although there are preferred regions for insertion, these consist of multiple integration points within a small area, as demonstrated by pairwise crosses and restriction mapping. Sequence analysis of three Tn9 insertions reveals that Tn9 integration is associated with a direct repeat of 9 base pairs (bp) of host sequence. We show that these extra 9 nucleotide pairs are generated upon insertion and not brought in with the element.

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Research works printed but not linked: 2

Auerswald, 1978, Insertion of transposon Tn5 results in a nine-base-pair duplication at the insertion site, Mol. Gen. Genet.
Johnsrud, 1978, DNA sequence of IS1, a transposable element, Mol. Gen. Genet.

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INTEGRATION OF TRANSPOSABLE DNA ELEMENTS: ANALYSIS BY DNA SEQUENCING11This work was supported by grants from the National Science Foundation and the National Institutes of Health. GC holds a postdoctoral fellowship from Medical Research Council of Canada. A. I. B, holds a Career Development Award of the National Institutes of Health.
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Authors: Johnsrud, L., Calos, M. P., & Miller, J. H.
Affiliations: Harvard University (US)
University of Geneva (CH)
ORCID: Miller, Jeffrey H.
Venue: Cell
Publisher: Elsevier BV
Published: 1978-12
Volume: 15
Issue: 4
Pages: 1209-1219
ISSN: 0092-8674 (ISSN-L)
DOI: 10.1016/0092-8674(78)90047-8
OpenAlex: W2078532746
Cited by: 58
References: 44
Citations by year: 2024: 1 · 2019: 1 · 2016: 1 · 2015: 1
FWCI: 12.22
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Cell (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
Keywords: transposon insertion, lac operon, Escherichia coli, transposon insertion sites
MeSH terms: Base Sequence, Chromosome Deletion, Chromosome Mapping, DNA Restriction Enzymes, Escherichia coli, Lac Operon, Translocation, Genetic

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The transposon Tn9 generates a 9 bp repeated sequence during integration

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