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The transposon Tn9 generates a 9 bp repeated sequence during integration
Abstract
We performed a genetic and sequencing analysis of insertions of the transposon Tn9 into the lac operon of E. coli. Genetic mapping of 70 insertions into lacl and Z shows that starting from the same point on the chromosome, Tn9 goes to at least 50 different points in these two genes. Although there are preferred regions for insertion, these consist of multiple integration points within a small area, as demonstrated by pairwise crosses and restriction mapping. Sequence analysis of three Tn9 insertions reveals that Tn9 integration is associated with a direct repeat of 9 base pairs (bp) of host sequence. We show that these extra 9 nucleotide pairs are generated upon insertion and not brought in with the element.
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“In some eubacteria and archaea, such as Acinetobacter baumannii, Escherichia coli, Enterococcus faecium, Lactococcus lactis, and Mycobacterium smegmatis, transposon-mediated LGT usually generates a pair of DRs varying from several to a dozen base pairs flanking the foreign gene after its integration into the host genome.33–36”
From Whole genomic DNA sequencing and comparative genomic analysis of Arthrospira platensis: high genome plasticity and genetic diversity (2016) · cites this paper as [33]
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Metadata
- Authors
- Johnsrud, L., Calos, M. P., & Miller, J. H.
- Affiliations
- Harvard University (US)
- University of Geneva (CH)
- ORCID
- Miller, Jeffrey H.
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1978-12
- Volume
- 15
- Issue
- 4
- Pages
- 1209-1219
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W2078532746
- Cited by
- 58
- References
- 44
- Citations by year
- 2024: 1 · 2019: 1 · 2016: 1 · 2015: 1
- FWCI
- 12.22
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Chromosomal and Genetic Variations
- Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
- Keywords
- transposon insertion, lac operon, Escherichia coli, transposon insertion sites
- MeSH terms
- Base Sequence, Chromosome Deletion, Chromosome Mapping, DNA Restriction Enzymes, Escherichia coli, Lac Operon, Translocation, Genetic
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