The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
DNA repair in an active gene: Removal of pyrimidine dimers from the DHFR gene of CHO cells is much more efficient than in the genome overall
Abstract
DNA repair was measured in the dihydrofolate reductase gene in Chinese hamster ovary cells, amplified for the gene, by quantitating pyrimidine dimers with a specific UV-endonuclease. More than two thirds of the dimers had been removed from a 14.1 kb restriction fragment of the gene by 26 hr after irradiation (20 J/m2), while little removal was detected in fragments upstream of the gene and only 15% were removed from the genome overall. This suggests that damage processing can vary according to function or activity of affected sequences, which has general implications for correlations of DNA repair with survival and mutagenesis. Perhaps preferential repair of vital sequences facilitates UV-resistance of these cells despite low overall repair levels.
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“XPA is an indispensable factor both for transcription-coupled NER and global genome NER [7], [8].”
From UV-Induced Nuclear Import of XPA Is Mediated by Importin-α4 in An ATR-Dependent Manner (2013) · cites this paper as [7]
In the citing paper
“Two subpathways of NER exist: transcription-blocking lesions in the transcribed strand (TS) of active genes are repaired quickly by transcription-coupled NER (TC-NER), whereas the rest of the genome, including the nontranscribed strand (NTS) of active genes, is repaired more slowly by global genome NER (GG-NER) (8, 33, 34, 44, 54).”
From A Role for Checkpoint Kinase-Dependent Rad26 Phosphorylation in Transcription-Coupled DNA Repair in Saccharomyces cerevisiae (2010) · cites this paper as [8]
Citations
Cites
Detection of specific sequences among DNA fragments separated by gel electrophoresis
Southern, E. M. (1975) · Journal of Molecular Biology · doi:10.1016/s0022-2836(75)80083-0 · Cited by 32,245
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Wahl, G. M., Stern, M., & Stark, G. R. (1979) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.76.8.3683 · Cited by 3,159
Calcium-dependent bacteriophage DNA infection
Mandel, M., & Higa, A. (1970) · Journal of Molecular Biology · doi:10.1016/0022-2836(70)90051-3 · Cited by 2,521
[29] Analysis of repeating DNA sequences by reassociation
Britten, R. J., Graham, D. E., & Neufeld, B. R. (1974) · Methods in enzymology on CD-ROM/Methods in enzymology · doi:10.1016/0076-6879(74)29033-5 · Cited by 1,041
Alt, F. W., Kellems, R. E., Bertino, J. R., & SCHIMKE, R. T. (1978) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(17)34875-5 · Cited by 934
DNA Repair in Bacteria and Mammalian Cells
Hanawalt, P. C., Cooper, P. K., Ganesan, A. K., & Smith, C. A. (1979) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.48.070179.004031 · Cited by 837
Gene amplification in cultured animal cells
SCHIMKE, R. T. (1984) · Cell · doi:10.1016/0092-8674(84)90406-9 · Cited by 635
Practical Methods in Molecular Biology
Schleif, R., & Wensink, P. C. (1981) · Springer eBooks · doi:10.1007/978-1-4612-5956-5 · Cited by 473
Sequence organization of the human genome
Schmid, C. W., & Deininger, P. L. (1975) · Cell · doi:10.1016/0092-8674(75)90184-1 · Cited by 301
Deletion of the diploid dihydrofolate reductase locus from cultured mammalian cells
Urlaub, G., Käs, E., Carothers, A. M., & Chasin, L. A. (1983) · Cell · doi:10.1016/0092-8674(83)90422-1 · Cited by 282
Human Diseases Associated with Defective DNA Repair
Friedberg, E. C., Ehmann, U. K., & Williams, J. I. (1979) · Advances in radiation biology · doi:10.1016/b978-0-12-035408-5.50008-3 · Cited by 218
Brown, P. C., Tlsty, T. D., & SCHIMKE, R. T. (1983) · Molecular and Cellular Biology · doi:10.1128/mcb.3.6.1097-1107.1983 · Cited by 190
Fujiwara, Y., & Tatsumi, M. (1976) · Mutation Research · doi:10.1016/0027-5107(76)90058-0 · Cited by 142
Amplification and loss of dihydrofolate reductase genes in a Chinese hamster ovary cell line.
Kaufman, R. J., & SCHIMKE, R. T. (1981) · Molecular and Cellular Biology · doi:10.1128/mcb.1.12.1069 · Cited by 141
Brown, P. C., Beverley, S. M., & SCHIMKE, R. T. (1981) · Molecular and Cellular Biology · doi:10.1128/mcb.1.12.1077 · Cited by 125
van Zeeland, A. A., Smith, C. A., & Hanawalt, P. C. (1981) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/0027-5107(81)90148-2 · Cited by 124
Expression of abbreviated mouse dihydrofolate reductase genes in cultured hamster cells.
Gasser, C. S., Simonsen, C. C., Schilling, J., & SCHIMKE, R. T. (1982) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.79.21.6522 · Cited by 106
Johnston, R. N., Beverley, S. M., & SCHIMKE, R. T. (1983) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.80.12.3711 · Cited by 105
Zelle, B., Reynolds, R. J., Kottenhagen, M. J., Luiten-Schuite, A., & Lohman, P. H. M. (1980) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/0027-5107(80)90121-9 · Cited by 99
Deficient repair of chemical adducts in α DNA of monkey cells
Zolan, M. E., Cortopassi, G. A., Smith, C. A., & Hanawalt, P. C. (1982) · Cell · doi:10.1016/0092-8674(82)90216-1 · Cited by 99
S phase-specific synthesis of dihydrofolate reductase in Chinese hamster ovary cells.
Mariani, B. D., Slate, D. L., & Schimke, R. (1981) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.78.8.4985 · Cited by 94
Structure of the dihydrofolate reductase gene in Chinese hamster ovary cells
Carothers, A. M., Urlaub, G., Ellis, N. A., & Chasin, L. A. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.7.1997 · Cited by 77
Measurement of repair replication by equilibrium sedimentation
Smith, C. A., Cooper, P. K., & Hanawalt, P. C. (1981) · OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) · Cited by 74
Mullenders, L. H. F., van Kesteren, A. C. A., Bussmann, C. J. M., van Zeeland, A. A., & Natarajan, A. T. (1984) · Mutation Research Letters · doi:10.1016/0165-7992(84)90014-9 · Cited by 70
Quantitation of aflatoxin B1 adduction within the ribosomal RNA gene sequences of rat liver DNA.
Irvin, T. R., & Wogan, G. N. (1984) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.81.3.664 · Cited by 54
Benzpyrene groups bind preferentially to the DNA of active chromatin in human lung cells
Arrand, J. E., & Murray, A. M. (1982) · Nucleic Acids Research · doi:10.1093/nar/10.5.1547 · Cited by 50
Nose, K., & Nikaido, O. (1984) · Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression · doi:10.1016/0167-4781(84)90093-9 · Cited by 46
Zolan, M. E., Smith, C. A., & Hanawalt, P. C. (1984) · Biochemistry · doi:10.1021/bi00296a010 · Cited by 46
Yu, F. (1983) · Carcinogenesis · doi:10.1093/carcin/4.7.889 · Cited by 44
Mechanisms of tolerance to DNA lesions in mammalian cells
Meneghini, R., Menck, C. F. M., & Schumacher, R. I. (1981) · Quarterly Reviews of Biophysics · doi:10.1017/s0033583500002353 · Cited by 42
Restricted repair of aflatoxin B1Induced damage In α DNA of monkey cells
Leadon, S. A., Zolan, M. E., & Hanawalt, P. C. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.16.5675 · Cited by 36
Mayne, L. V. (1984) · Mutation Research/DNA Repair Reports · doi:10.1016/0167-8817(84)90023-3 · Cited by 24
Factors that affect the initiation of excision-repair in chromatin.
Bohr, V. A., & Hanawalt, P. C. (1984) · PubMed · Cited by 10
Self-citation
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Sorted by citations
Cellular processing of platinum anticancer drugs
Wang, D., & Lippard, S. J. (2005) · Nature Reviews Drug Discovery · doi:10.1038/nrd1691 · Cited by 3,801
Molecular Mechanisms of Mammalian DNA Repair and the DNA Damage Checkpoints
Sancar, A., Lindsey-Boltz, L. A., Ünsal-Kaçmaz, K., & Linn, S. (2004) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.73.011303.073723 · Cited by 3,317
MELLON, I. (1987) · Cell · doi:10.1016/0092-8674(87)90151-6 · Cited by 1,228
Transcription-coupled DNA repair: two decades of progress and surprises
Hanawalt, P. C., & Spivak, G. (2008) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2549 · Cited by 1,081
Ichihashi, M., Ueda, M., Budiyanto, A., Bito, T., Oka, M., Fukunaga, M., Tsuru, K., & Horikawa, T. (2003) · Toxicology · doi:10.1016/s0300-483x(03)00150-1 · Cited by 981
“It is reasonable to assume that mutations that only affect DNA repair function of XPD could lead to XP, whereas in XP /CS and TTD (at least) the transcription function is modified, and therefore, also the transcription-coupled repair (TCR) pathway (van Steeg and Kraemer, 1999).”
BackgroundInfluential
Metadata
- Authors
- Bohr, V
- Affiliations
- Stanford University (US)
- Corresponding author
- Bohr, V
- ORCID
- Bohr, V
- Venue
- Cell
- Publisher
- Elsevier BV
- Published
- 1985-02
- Volume
- 40
- Issue
- 2
- Pages
- 359-369
- ISSN
- 0092-8674 (ISSN-L)
- OpenAlex
- W1984353960
- Cited by
- 1,237
- References
- 34
- Citations by year
- 2026: 11 · 2025: 17 · 2024: 15 · 2023: 14 · 2022: 12 · 2021: 17 · 2020: 18 · 2019: 11 · 2018: 18 · 2017: 13 · 2016: 23 · 2015: 9 · 2014: 24 · 2013: 24 · 2012: 22
- FWCI
- 15.13
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Cell (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institute of General Medical Sciences (GM 09901)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Carcinogens and Genotoxicity Assessment
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Effects of Radiation Exposure
- Keywords
- DNA repair, pyrimidine dimers, dihydrofolate reductase gene, UV-induced DNA damage, UV endonuclease, UV resistance, CHO cells, mutagenesis, cell survival
- MeSH terms
- Animals, Base Sequence, Cell Line, Cell Survival, Cricetulus, DNA Repair, Endodeoxyribonucleases, Female, Cricetinae, Multienzyme Complexes, N-Glycosyl Hydrolases, Pyrimidine Dimers, Tetrahydrofolate Dehydrogenase
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